BGMUT Database of Allelic Variants of Genes Encoding Human Blood Group Antigens

Patnaik, Santosh K.; Helmberg, Wolfgang; Blumenfeld, Olga O.

doi:10.1159/000366108

Cited by 39 publications

(38 citation statements)

References 19 publications

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“…Moreover, bioconjugates remained fluorescent, stable, and specifically labeling the cells for at least 6 months. This was confirmed by the results obtained after incubating A 1 …”

Section: Resultssupporting

confidence: 79%

“…1 These immunohematological molecular analyses have been contributing to the study of genes related to antigens and also to the understanding of a great number of polymorphisms; 2,3 nevertheless, they do not provide information regarding quantitative analyses, nor distribution, of the antigens on red blood cell (RBC) membrane surfaces. 4 Analyses of antigen expressions are to this day performed by using methods based on hemagglutination, which can be performed in tube, microplate, and gel, with different potentiating reaction solutions.…”

Section: Introductionmentioning

confidence: 99%

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Blood group antigen studies using CdTe quantum dots and flow cytometry

Filho¹,

Pereira²,

Fernandes³

et al. 2015

IJN

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New methods of analysis involving semiconductor nanocrystals (quantum dots [QDs]) as fluorescent probes have been highlighted in life science. QDs present some advantages when compared to organic dyes, such as size-tunable emission spectra, broad absorption bands, and principally exceptional resistance to photobleaching. Methods applying QDs can be simple, not laborious, and can present high sensibility, allowing biomolecule identification and quantification with high specificity. In this context, the aim of this work was to apply dual-color CdTe QDs to quantify red blood cell (RBC) antigen expression on cell surface by flow cytometric analysis. QDs were conjugated to anti-A or anti-B monoclonal antibodies, as well as to the anti-H ( Ulex europaeus I) lectin, to investigate RBCs of A 1 , B, A 1 B, O, A 2 , and A weak donors. Bioconjugates were capable of distinguishing the different expressions of RBC antigens, both by labeling efficiency and by flow cytometry histogram profile. Furthermore, results showed that RBCs from A weak donors present fewer amounts of A antigens and higher amounts of H, when compared to A 1 RBCs. In the A group, the amount of A antigens decreased as A 1 > A 3 > A X = A el , while H antigens were A X = A el > A 1 . Bioconjugates presented stability and remained active for at least 6 months. In conclusion, this methodology with high sensibility and specificity can be applied to study a variety of RBC antigens, and, as a quantitative tool, can help in achieving a better comprehension of the antigen expression patterns on RBC membranes.

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“…Moreover, bioconjugates remained fluorescent, stable, and specifically labeling the cells for at least 6 months. This was confirmed by the results obtained after incubating A 1 …”

Section: Resultssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Blood group antigen studies using CdTe quantum dots and flow cytometry

Filho¹,

Pereira²,

Fernandes³

et al. 2015

IJN

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show abstract

“…Therefore, accurate and reliable typing of blood groups is an essential guarantee for safe blood transfusion. 6,7 The ABO blood groups are often identified by detecting the antigen on the surface of red blood cells (RBCs), and various detection methods are used for blood typing.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Zhang

Fan

et al. 2017

IJN

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Background Accurate and reliable blood grouping is essential for safe blood transfusion. However, conventional methods are qualitative and use only single-antigen detection. We overcame these limitations by developing a simple, quantitative, and multiplexed detection method for blood grouping using quantum dots (QDs) and magnetic beads. Methods In the QD fluorescence assay (QFA), blood group A and B antigens were quantified using QD labeling and magnetic beads, and the blood groups were identified according to the R value (the value was calculated with the fluorescence intensity from dual QD labeling) of A and B antigens. The optimized performance of QFA was established by blood typing 791 clinical samples. Results Quantitative and multiplexed detection for blood group antigens can be completed within 35 min with more than 10 5 red blood cells. When conditions are optimized, the assay performance is satisfactory for weak samples. The coefficients of variation between and within days were less than 10% and the reproducibility was good. The ABO blood groups of 791 clinical samples were identified by QFA, and the accuracy obtained was 100% compared with the tube test. Receiver-operating characteristic curves revealed that the QFA has high sensitivity and specificity toward clinical samples, and the cutoff points of the R value of A and B antigens were 1.483 and 1.576, respectively. Conclusion In this study, we reported a novel quantitative and multiplexed method for the identification of ABO blood groups and presented an effective alternative for quantitative blood typing. This method can be used as an effective tool to improve blood typing and further guarantee clinical transfusion safety.

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“…We utilize the publicly available data set Blood Group Antigen Gene Mutation Database (BGMUT), which is an online repository of the allelic variations in genes that determine the antigens of various human blood group systems [22]. The data set is in VCF file format.…”

Section: B Genome Data Sets and Genomic Rulesmentioning

confidence: 99%

Practical personalized genomics in the encrypted domain

Singh

Sirdey

Carpov

2018

2018 Third International Conference on Fog and Mobile Edge Computing (FMEC)

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Abstract-In this paper, we examine and propose a solution for the challenges of sharing of genome sequence data and of data querying on the genome sequence data on a cloud server in personalized medicine scenarios. We develop a privacy-preserving, a secure and efficient solution for personalized medicine. The solution that we propose making use of stream cipher-based homomorphic transciphering in a cloud server, and to show the effectiveness of transciphering solution in the personalized medicine scenario. This paper also provides the comparative analysis of well-known existing homomorphic encryption solutions BGV and FV schemes combined with the FLIP stream cipher to demonstrate the efficiency and privacy of our solution.

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BGMUT Database of Allelic Variants of Genes Encoding Human Blood Group Antigens

Cited by 39 publications

References 19 publications

Blood group antigen studies using CdTe quantum dots and flow cytometry

Blood group antigen studies using CdTe quantum dots and flow cytometry

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Practical personalized genomics in the encrypted domain

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BGMUT Database of Allelic Variants of Genes Encoding Human Blood Group Antigens

Cited by 39 publications

References 19 publications

Blood group antigen studies using CdTe quantum dots and flow cytometry

Blood group antigen studies using CdTe quantum dots and flow cytometry

Quantitative and multiplexed detection for blood typing based on quantum dot&ndash;magnetic bead assay

Practical personalized genomics in the encrypted domain

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Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay