Bi-directional encoding of context-based odors and behavioral states by the nucleus of the lateral olfactory tract

Tanisumi, Yuta; Shiotani, Kazuki; Hirokawa, Junya; Sakurai, Yoshio; Manabe, Hiroyuki

doi:10.1016/j.isci.2021.102381

Cited by 9 publications

(10 citation statements)

References 46 publications

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“…Detecting task-related neurons: To evaluate the task-related neurons, we computed peri-stimulus time histograms using a 20 ms bin width and smoothed by convolving spike trains with a 40-ms wide Gaussian filter in four trial outcome conditions (Rc, right-correct; Re, right-erroneous; Lc, left-correct; and Le, left-erroneous; Figure 1B ). For each neuron, we performed a sliding ROC analysis ( Shiotani et al, 2020 ; Tanisumi et al, 2021 ). Starting from the baseline period of each condition (4 s of fixation before trial start), an ROC value was calculated for a 100 ms bin.…”

Section: Methodsmentioning

confidence: 99%

Hippocampal CA1 Neurons Represent Positive Feedback During the Learning Process of an Associative Memory Task

Takamiya

Shiotani

Ohnuki

et al. 2021

Front. Syst. Neurosci.

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The hippocampus is crucial for forming associations between environmental stimuli. However, it is unclear how neural activities of hippocampal neurons dynamically change during the learning process. To address this question, we developed an associative memory task for rats with auditory stimuli. In this task, the rats were required to associate tone pitches (high and low) and ports (right and left) to obtain a reward. We recorded the firing activity of neurons in rats hippocampal CA1 during the learning process of the task. As a result, many hippocampal CA1 neurons increased their firing rates when the rats received a reward after choosing either the left or right port. We referred to these cells as “reward-direction cells.” Furthermore, the proportion of the reward-direction cells increased in the middle-stage of learning but decreased after the completion of learning. This result suggests that the activity of reward-direction cells might serve as “positive feedback” signal that facilitates the formation of associations between tone pitches and port choice.

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Section: Methodsmentioning

confidence: 99%

Hippocampal CA1 Neurons Represent Positive Feedback During the Learning Process of an Associative Memory Task

Takamiya

Shiotani

Ohnuki

et al. 2021

Front. Syst. Neurosci.

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“…On the other hand, inputs from olfactory cortical regions (PC and TT/AON) are powerful and can drive NLOT bursting. This suggests that NLOT activity primarily reflects highly processed olfactory information (Tanisumi et al, 2021). Interestingly, similar findings were described for olfactory inputs into the medial amygdala (Keshavarzi et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Synaptic connectivity and electrophysiological properties of the nucleus of the lateral olfactory tract

Penker,

Lawabny,

Dhamshy

et al. 2024

Preprint

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The sense of smell is tightly linked to emotions, a link that is thought to rely on the direct synaptic connections between the olfactory bulb and nuclei of the amygdala. A small number of amygdaloid nuclei are the recipients of such direct input from the olfactory bulb and their unique functions are not known. Among them, the nucleus of the lateral olfactory tract (NLOT) is unique in its developmental history and gene expression. NLOT has been very little studied and consequentially its function is unknown. Furthermore, formulation of informed hypotheses about NLOT function is at this stage limited by the lack of knowledge about its connectivity and physiological properties. Here, we used pseudo-rabies tracing methods to systematically reveal monosynaptic inputs into NLOT, and adeno-associated viruses to reveal NLOT projection targets. We found that the NLOT is interconnected with several olfactory brain regions and with the basolateral amygdala. Some of these connections were reciprocal, and some showed unique interhemispheric patterns. We tested the excitable properties of NLOT neurons and the properties of each of the major synaptic inputs. We found that the NLOT receives powerful input from piriform cortex, tenia tecta, and the basolateral amygdala, but only very weak input from the olfactory bulb. When input crosses threshold, NLOT neurons respond with calcium-dependent bursts of action potentials. This integration of olfactory and amygdalar inputs suggests that NLOT plays a role in behaviors that combine smell and emotion, possibly assigning emotional value to odors.

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“…In the cortical amygdala, we found distinct anterior and posterior types of Trh neurons, previously studied as one population, and other, novel anterior types defined by Tgfb2 , and others. In the nucleus of the lateral olfactory tract, we identified and validated a large population of molecularly distinct Sim1-C1ql1 neurons (GABA 8) that may be involved in associating context-based odor information with behavior 66,67 .…”

Section: Discussionmentioning

confidence: 99%

Cell types in the mouse amygdala and their transcriptional response to fear conditioning

Hochgerner

Tibi

Netser

et al. 2022

Preprint

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The amygdala is one of the most widely studied regions in behavioral neuroscience. A plethora of classical, and new paradigms have dissected its precise involvement in emotional and social sensing, learning, and memory. Several important insights resulted from the use of genetic markers - yet, in the age of single cell transcriptomics, the amygdala remains molecularly underdescribed. Here, we present a molecular cell type taxonomy of the full mouse amygdala in fear learning and consolidation. We performed single-cell RNA-seq on naïve and fear conditioned mice, inferred the 130 neuronal cell types distributions in silico using orthogonal spatial transcriptomic datasets, and describe the cell types' transcriptional responses to learning and memory consolidation. Only a fraction of cells, within a subset of all neuronal types, were transcriptionally responsive to fear learning, memory and retrieval. These activated engram cells upregulated activity-response genes, and processes of synaptic signaling, plasticity, development and neurite outgrowth. Our transcriptome-wide data confirm known actors, and describe several new candidate genes. The atlas may help pinpoint the amygdala's circuits in performing emotional sensing and integration, and provide new insights to the global cellular processes involved.

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Bi-directional encoding of context-based odors and behavioral states by the nucleus of the lateral olfactory tract

Cited by 9 publications

References 46 publications

Hippocampal CA1 Neurons Represent Positive Feedback During the Learning Process of an Associative Memory Task

Hippocampal CA1 Neurons Represent Positive Feedback During the Learning Process of an Associative Memory Task

Synaptic connectivity and electrophysiological properties of the nucleus of the lateral olfactory tract

Cell types in the mouse amygdala and their transcriptional response to fear conditioning

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