Bi-directional modulation of T cell-dependent antibody production by prostaglandin E2

Abstract

T cell-dependent Ig production involves interaction between T cells and B cells. This study evaluated the effects of prostaglandin (PG) E(2) on Ig production in a system in which B cells were co-cultured with autologous CD4(+) T cell clones non-specifically activated by anti-CD3. The effects of PGE(2) on T cell-dependent Ig production differed substantially, depending on the T cells employed. We selected six T cell clones that were able to enhance Ig production (resistant T cell clones) and six T cell clones that inhibited Ig production in the presence of PGE(2) (sensitive T cell clones) for comparison. The resistant T cells produced high levels (>1000 pg/ml) of IL-2 and/or IL-4, and expressed high CD40L, OX40 and CD45RA, and low CD45RO. In contrast, sensitive T cells secreted low IL-2 (<500 pg/ml) and IL-4 (<200 pg/ml), and expressed low CD40, OX40 and CD45RA, and high CD45RO. Adding supernatant derived from resistant T cell clones restored Ig production inhibited by PGE(2), while removing IL-2, IL-4 or IL-10 using specific antibodies inhibited Ig production. In addition, we demonstrated a direct effect of PGE(2) on B cells to enhance Ig production. Consistently, in the presence of resistant T cells, PGE(2) increased B cell proliferation and differentiation. In conclusion, the effects of PGE(2) on Ig production consist of its indirect effects through T cells and its direct effects on B cells. The outcome of the effects can be up-regulatory or down-regulatory, depending whether resistant or sensitive T cells are involved.

Fontes de óleo na dieta e sua influência no desempenho e na imunidade de frangos de corte

“…Numa dieta rica em PUFAS n-3, estes são incorporados às membranas celulares substituindo o ácido araquidônico, e a consequência dessa substituição é a menor produção de eicosanoides mediadores, como a PGE2 e o LTB4. A PGE2 poderá, em diferentes situações, inibir ou estimular a produção de imunoglobulinas pelas células B, através do estímulo à produção e interação de diferentes citocinas (He et al 2001).…”

Activated Human B Lymphocytes Express Cyclooxygenase-2 and Cyclooxygenase Inhibitors Attenuate Antibody Production

“…4B, indomethacin blocks the production of nearly all the PGE 2 and PGF 2␣ . Treatment with the Cox-2-selective inhibitor, SC-58125, resulted in ϳ85% decrease in PGE 2 and PGF 2␣ produced, indicating that the majority of PGE 2 and PGF 2␣ were produced by the inducible Cox-2 (Fig. 4B).…”

“…B cells were stimulated with nothing or with recombinant human CD40L (8), pansorbin (Staphylococcus aureus Cowen I strain; Sigma-Aldrich), or rabbit antihuman F(abЈ) 2 anti-IgM Ab (Jackson ImmunoResearch Laboratories). Arachidonic acid, (Nu-Chek Prep) dissolved in 100% ethanol, was diluted to working concentrations in culture medium.…”

“…Freshly isolated B cells were incubated with and without the nonspecific B cell activator pansorbin or with CD40L Ϯ anti-IgM Ab for 36 h. The supernatants were then assayed for PGE 2 and PGF 2␣ using a specific EIA analysis. In Fig.…”

A Cyclooxygenase-2/Prostaglandin E2 Pathway Augments Activation-Induced Cytosine Deaminase Expression within Replicating Human B Cells

“…Although diminished viability of replicating progeny may, in part, explain these findings, there is also evidence that COX-2 influences B cell Ig class-switch recombination. Supplementing mouse B cell cultures with PGE 2 significantly augments LPS + IL-4-induced production of є-germline transcripts (10), rearranged VDJ-ε transcripts (11), and secretion of IgG and IgE (10,12,13).…”