Bi-directional PCR allele-specific amplification (bi-PASA) for detection of caspase-8 −652 6N ins/del promoter polymorphism (rs3834129) in breast cancer

Hashemi, Mohammad; Eskandari-Nasab, Ebrahim; Fazaeli, Aliakbar; Rezaei, Hamzeh; Mashhadi, Mohammad Ali; Arbabi, Farshid; Taheri, Mohsen

doi:10.1016/j.gene.2012.05.043

Cited by 35 publications

(21 citation statements)

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“…Similarly, BC is the most common malignancy affecting Iranian women (2). Although the etiology of BC remains to be identified, genetic factors are shown to have important roles in the pathogenesis and progress of this malignancy (3)(4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the pri-miR-34b/c rs4938723 polymorphism and its association with breast cancer risk

et al. 2016

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Abstract. MicroRNAs (miRNAs or miRs) are a family of small non-coding RNAs that function as oncogenes or tumor suppressor genes. Recent evidence suggests that the pri-miR-34b/c rs4938723 variant is associated with the development of cancer. At present, there is an inconsistent association between the single-nucleotide polymorphism in pri-miR-34b/c and cancer in the limited studies. The present study is a case-control investigation, with 263 breast cancer (BC) patients and 221 control women, which examined the potential association of the pri-miR-34b/c rs4938723 polymorphisms with BC susceptibility. The polymorphisms were genotyped by the polymerase chain reaction restriction fragment length polymorphism method. No significant association between the pri-miR-34b/c rs4938723 variant and BC was identified [TC vs. TT: Odds ratio (OR), 0.87; 95% confidence interval (CI), 0.60-1.26; P=0.506; CC vs. TT: OR, 1.22; 95% CI, 0.61-2.47; P= 0.600; TC+CC vs. TT: OR, 0.91; 95% CI, 0.64-1.31; P= 0.648; CC vs. TT+TC: OR, 1.32; 95% CI, 0.67-2.59; P= 0.498; C vs. T: OR, 0.99; 95% CI, 0.75-1.31; P= 0.986]. However, a significant association was observed between the pri-miR-34b/c rs4938723 genotypes and clinicopathological characteristics, such a grade, progesterone receptor and human epidermal growth factor receptor 2 status were observed (P<0.05). These findings suggest that the pri-miR-34b/c rs4938723 variant may not be a risk factor for the development of BC. IntroductionBreast cancer (BC), the most prevalent type of cancer in women, is a major public and global health problem and accounts for 14% of total annual cancer fatalities worldwide (1). Similarly, BC is the most common malignancy affecting Iranian women (2). Although the etiology of BC remains to be identified, genetic factors are shown to have important roles in the pathogenesis and progress of this malignancy (3-8).MicroRNAs (miRNAs or miRs) are a class of single-stranded non-coding RNA typically 17-25 nucleotides in length that have key roles in the regulation of cellular processes by targeting mRNAs for cleavage or translational repression (9,10). They regulate the expression of genes at the post-transcriptional level by targeting the 3' untranslated regions of mRNAs. Cumulative evidence suggests that the dysregulation of miRNA expression is involved in the tumorigenesis by acting as tumor suppressors or oncogenes (11-15). Single-nucleotide polymorphisms (SNPs) or mutations in miRNA genes can affect the miRNA biosynthesis and expression of target genes, therefore resulting in diverse functional consequences and thereby possibly representing potentially important biomarkers for the prognosis of cancer (7,(16)(17)(18).The pri-miR-34b/c gene resides in a CpG island within the intron of the B-cell translocation gene 4. Therefore, it is expected that pri-miR-34b/c is co-transcribed by either the promoter of the protein-coding gene or by its own transcription initiation region, as is the case with the majority of miRNAs, whether they are intergenic or located within the i...

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Section: Introductionmentioning

confidence: 99%

Evaluation of the pri-miR-34b/c rs4938723 polymorphism and its association with breast cancer risk

et al. 2016

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“…Cancer has been reported as the third cause of death in Iran after coronary heart disease and accidents (Harirchi et al, 2011), and among all malignancies, BC is the most common cancer affecting Iranian women . Although the etiology of BC is unknown, genetic factors are shown to play important roles in the pathogenesis and progress of this malignancy (Eskandari-Nasab et al, 2012;Hashemi et al, 2012aHashemi et al, ,b, 2013.…”

Section: Introductionmentioning

confidence: 99%

Effect of TP53 16-bp and β-TrCP 9-bp INS/DEL polymorphisms in relation to risk of breast cancer

Eskandari-Nasab

Hashemi

Amininia

et al. 2015

Gene

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“…All the extracts, described earlier in the cytotoxicity assay, inhibited cell proliferation relative to the control, as revealed by differential expression of apoptosis related gene family in the transcriptomes. Apoptosis is a critical defense against hyperproliferation of cancer [42] with the results potentially indicative of reduced cell viability with ensuing metabolic processes potentially precipitating apoptosis and necrosis. This is further underpinned by significant induction of caspases namely casp8 and casp8AP2 in this study (Table 3) which are established executioners of apoptosis cascades.…”

Section: Resultsmentioning

confidence: 99%

Tea (Camellia sinensis) infusions ameliorate cancer in 4TI metastatic breast cancer model

Mbuthia

Mireji

Ngure

et al. 2017

BMC Complement Altern Med

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BackgroundTea (Camellia sinensis) infusions are widely consumed beverages with numerous health benefits. However, physiological and molecular responses mediating these activities are poorly understood.MethodThree replicates of 4TI cancer cell suspension (2.0 × 105 cells/ml) were challenged in vitro with various concentrations of green, black and purple tea infusions to asseses their cytoxicity and associated differentially expressed genes in the cells. Inhibitory activity was tested by using serial dilutions of respective tea infusions in a 96 well ELISA plate.ResultsGreen tea had the highest inhibition on 4TI cells proliferation at a concentration of IC50 = 13.12 μg/ml. Further analysis of the 4TI cancer cell line treated with tea using 454 pyrosequencing generated 425,696 reads with an input mean length of 286.54. Trimmed sequences were imported on a CLC genomic workbench v7.03 and annotated on a reference mouse genome (Mus musculus strain C57BL/6 J). Results revealed a differential expression of apoptosis related genes in the transcriptome. Casp8, Casp9, Casp3, Casp6, Casp8AP2, Aifm1, Aifm2 and Apopt1 genes were significantly upregulated indicating the process of apoptosis was initiated and executed.ConclusionThese findings on caspases offer valuable information on the mechanism of tea as an anticancer agent and will contribute to further research in future novel treatments.

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Bi-directional PCR allele-specific amplification (bi-PASA) for detection of caspase-8 −652 6N ins/del promoter polymorphism (rs3834129) in breast cancer

Cited by 35 publications

References 30 publications

Evaluation of the pri-miR-34b/c rs4938723 polymorphism and its association with breast cancer risk

Evaluation of the pri-miR-34b/c rs4938723 polymorphism and its association with breast cancer risk

Effect of TP53 16-bp and β-TrCP 9-bp INS/DEL polymorphisms in relation to risk of breast cancer

Tea (Camellia sinensis) infusions ameliorate cancer in 4TI metastatic breast cancer model

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