Biaxial biomechanical properties of self-assembly tissue-engineered blood vessels

Zaucha, Michael T.; Gauvin, Robert; Auger, François A.; Germain, Lucie; Gleason, Rudolph L.

doi:10.1098/rsif.2010.0228

Cited by 12 publications

(9 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed physiologically relevant tangent moduli in our single‐layer VSMC sheets supports our proposed approach for constructing thicker arterial tissue by stacking multiple patterned VSMC sheets. It is interesting to note that compared to other tissue‐engineered vascular tissue, our VSMC sheets have tangent moduli smaller than aligned VSMC sheets cultured for longer durations (8–10 weeks) as well as anisotropic, self‐assembled engineered tissue constructs grown using fibroblasts …”

Section: Resultsmentioning

confidence: 99%

A Robust Method to Generate Mechanically Anisotropic Vascular Smooth Muscle Cell Sheets for Vascular Tissue Engineering

Backman

LeSavage

Shah

et al. 2017

Macromolecular Bioscience

View full text Add to dashboard Cite

In arterial tissue engineering, mimicking native structure and mechanical properties is essential because compliance mismatch can lead to graft failure and further disease. With bottom-up tissue engineering approaches, designing tissue components with proper microscale mechanical properties is crucial to achieve the necessary macroscale properties in the final implant. Here, we developed a thermo-responsive cell culture platform for growing aligned vascular smooth muscle cell sheets by photo-grafting N-isopropylacrylamide (NIPAAm) onto micro-patterned PDMS. We experimentally and computationally optimized the grafting process to produce PNIPAAm-PDMS substrates optimal for vascular smooth muscle cell (VSMC) attachment. To allow long-term VSMC sheet culture and increase the rate of VSMC sheet formation, PNIPAAm-PDMS surfaces were further modified with 3-aminopropyltriethoxysilane (APTES) yielding a robust, thermo-responsive cell culture platform for culturing VSMC sheets. VSMC cell sheets cultured on patterned thermo-responsive substrates exhibit cellular and collagen alignment in the direction of the micro-pattern. Mechanical characterization of patterned, single-layer VSMC sheets reveals increased stiffness in the aligned direction compared to the perpendicular direction whereas non-patterned cell sheets exhibit no directional dependence. Structural and mechanical anisotropy of aligned, single-layer VSMC sheets makes this platform an attractive micro-structural building block for engineering a vascular graft to match the in vivo mechanical properties of native arterial tissue.

show abstract

Section: Resultsmentioning

confidence: 99%

A Robust Method to Generate Mechanically Anisotropic Vascular Smooth Muscle Cell Sheets for Vascular Tissue Engineering

Backman

LeSavage

Shah

et al. 2017

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…Our results can also guide efforts on the development of new types of patches using tissue engineering and other methods [37,[56][57][58][59][60]. The large variability of the native carotid tissue documented in this work (with individual specimen loading curves and calculated anisotropy indices) raises the possibility of development of individualized patches that will be tailored to match tissue properties for a specific patient.…”

Section: Discussionmentioning

confidence: 73%

Comparative Analysis of the Biaxial Mechanical Behavior of Carotid Wall Tissue and Biological and Synthetic Materials Used for Carotid Patch Angioplasty

Kamenskiy

Pipinos

MacTaggart

et al. 2011

Journal of Biomechanical Engineering

View full text Add to dashboard Cite

Patch angioplasty is the most common technique used for the performance of carotid endarterectomy. A large number of patching materials are available for use while new materials are being continuously developed. Surprisingly little is known about the mechanical properties of these materials and how these properties compare with those of the carotid artery wall. Mismatch of the mechanical properties can produce mechanical and hemodynamic effects that may compromise the long-term patency of the endarterectomized arterial segment. The aim of this paper was to systematically evaluate and compare the biaxial mechanical behavior of the most commonly used patching materials. We compared PTFE (n = 1), Dacron (n = 2), bovine pericardium (n = 10), autogenous greater saphenous vein (n = 10), and autogenous external jugular vein (n = 9) with the wall of the common carotid artery (n = 18). All patching materials were found to be significantly stiffer than the carotid wall in both the longitudinal and circumferential directions. Synthetic patches demonstrated the most mismatch in stiffness values and vein patches the least mismatch in stiffness values compared to those of the native carotid artery. All biological materials, including the carotid artery, demonstrated substantial nonlinearity, anisotropy, and variability; however, the behavior of biological and biologically-derived patches was both qualitatively and quantitatively different from the behavior of the carotid wall. The majority of carotid arteries tested were stiffer in the circumferential direction, while the opposite anisotropy was observed for all types of vein patches and bovine pericardium. The rates of increase in the nonlinear stiffness over the physiological stress range were also different for the carotid and patching materials. Several carotid wall samples exhibited reverse anisotropy compared to the average behavior of the carotid tissue. A similar characteristic was observed for two of 19 vein patches. The obtained results quantify, for the first time, significant mechanical dissimilarity of the currently available patching materials and the carotid artery. The results can be used as guidance for designing more efficient patches with mechanical properties resembling those of the carotid wall. The presented systematic comparative mechanical analysis of the existing patching materials provides valuable information for patch selection in the daily practice of carotid surgery and can be used in future clinical studies comparing the efficacy of different patches in the performance of carotid endarterectomy.

show abstract

“…Over the years, multiple models have been developed to describe the 3D stress-strain relationship of blood vessels. 1,12,25,28,33 These models, based on the analysis of the mechanical behavior of explanted vascular tissues, are providing a quantitative and hierarchical way to predict blood vessel response to a dynamic physiological environment. [13][14][15] However, due to the complex data analysis behind these models, comparison between the parameters evaluated is very challenging and hard to translate into the design process of tissueengineered vascular constructs.…”

Section: Discussionmentioning

confidence: 99%

A Computer-Controlled Apparatus for the Characterization of Mechanical and Viscoelastic Properties of Tissue-Engineered Vascular Constructs

Lévesque

Gauvin

Larouche

et al. 2011

Cardiovasc Eng Tech

Self Cite

View full text Add to dashboard Cite

Tissue-engineered blood vessels can be partly characterized by analyzing their mechanical properties using burst pressure testing, compliance measurement, creep and cyclic testing. Studying these parameters provides information on the capability of a fabrication method to produce tissue-engineered blood vessels (TEBV) and allow for the optimization of their resistance and viscoelastic properties. This study presents the design and fabrication of an apparatus allowing accurate and reliable measurements of the mechanical properties of tissue-engineered vascular constructs. A computer-controlled system was designed to monitor pressure and diameter variations of vascular constructs submitted to hydrostatic loading. The system was programmed to control the motorized portion of the setup and allow simultaneous data acquisition, analysis and realtime display. Data acquisition cards allow for synchronous monitoring of pressure and diameter of the constructs through a pressure transducer and a CCD camera. Image analysis and pressure data computation resulted in compliance, creep and dynamic characterization of the tested tissues. This experimental setup succeeded in measuring the burst pressure, compliance, creep and cyclic behavior of tissue-engineered vascular media (TEVM), adventitia (TEVA) and a combination of a media and an adventitia (TEVMA) reconstructed by the self-assembly method. Our apparatus has proven to be a precise and reliable tool for the characterization of the mechanical properties of vascular constructs.

show abstract

Biaxial biomechanical properties of self-assembly tissue-engineered blood vessels

Cited by 12 publications

References 37 publications

A Robust Method to Generate Mechanically Anisotropic Vascular Smooth Muscle Cell Sheets for Vascular Tissue Engineering

A Robust Method to Generate Mechanically Anisotropic Vascular Smooth Muscle Cell Sheets for Vascular Tissue Engineering

Comparative Analysis of the Biaxial Mechanical Behavior of Carotid Wall Tissue and Biological and Synthetic Materials Used for Carotid Patch Angioplasty

A Computer-Controlled Apparatus for the Characterization of Mechanical and Viscoelastic Properties of Tissue-Engineered Vascular Constructs

Contact Info

Product

Resources

About