Biaxial tensile testing and constitutive modeling of human supraspinatus tendon

Szczesny, Spencer E.; Peloquin, John M.; Cortés, Daniel H.; Kadlowec, Jennifer; Soslowsky, Louis J.; Elliott, Dawn M.

doi:10.1109/nebc.2012.6206986

Cited by 7 publications

(11 citation statements)

References 39 publications

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“…[27][28][29] This was then used to calculate stretch in the fiber direction (fiber stretch). In this process, the fiber direction is defined by the initial direction of scaffold fiber alignment (a o ) through the vector:…”

Section: Biaxial Mechanics Of Angle Ply Laminatesmentioning

confidence: 99%

Biaxial mechanics and inter‐lamellar shearing of stem‐cell seeded electrospun angle‐ply laminates for annulus fibrosus tissue engineering

Driscoll

Nakasone

Szczesny

et al. 2013

Journal Orthopaedic Research

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The annulus fibrosus (AF) of the intervertebral disk plays a critical role in vertebral load transmission that is heavily dependent on the microscale structure and composition of the tissue. With degeneration, both structure and composition are compromised, resulting in a loss of AF mechanical function. Numerous tissue engineering strategies have addressed the issue of AF degeneration, but few have focused on recapitulation of AF microstructure and function. One approach that allows for generation of engineered AF with appropriate (þ/À)308 lamellar microstructure is the use of aligned electrospun scaffolds seeded with mesenchymal stem cells (MSCs) and assembled into angle-ply laminates (APL). Previous work indicates that opposing lamellar orientation is necessary for development of near native uniaxial tensile properties. However, most native AF tensile loads are applied biaxially, as the disk is subjected to multi-axial loads and is constrained by its attachments to the vertebral bodies. Thus, the objective of this study was to evaluate the biaxial mechanical response of engineered AF bilayers, and to determine the importance of opposing lamellar structure under this loading regime. Opposing bilayers, which replicate native AF structure, showed a significantly higher modulus in both testing directions compared to parallel bilayers, and reached $60% of native AF biaxial properties. Associated with this increase in biaxial properties, significantly less shear, and significantly higher stretch in the fiber direction, was observed. These results provide additional insight into native tissue structure-function relationships, as well as new benchmarks for engineering functional AF tissue constructs. ß

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Section: Biaxial Mechanics Of Angle Ply Laminatesmentioning

confidence: 99%

Biaxial mechanics and inter‐lamellar shearing of stem‐cell seeded electrospun angle‐ply laminates for annulus fibrosus tissue engineering

Driscoll

Nakasone

Szczesny

et al. 2013

Journal Orthopaedic Research

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“…PLM has been proven effective to characterize collagen of tendons [20,21], ligaments [22] and other soft tissues [23]. The possibility of using PLM in the cornea was discussed almost two decades ago for ex-vivo tissues [24], and more recently to study the cornea stroma in vivo [25].…”

Section: Introductionmentioning

confidence: 99%

Polarization microscopy for characterizing fiber orientation of ocular tissues

Jan

Grimm

Tran

et al. 2015

Biomed. Opt. Express

150

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Characterizing the collagen fiber orientation and organization in the eye is necessary for a complete understanding of ocular biomechanics. In this study, we assess the performance of polarized light microscopy to determine collagen fiber orientation of ocular tissues. Our results demonstrate that the method provides objective, accurate, repeatable and robust data on fiber orientation with µm-scale resolution over a broad, cmscale, field of view, unaffected by formalin fixation, without requiring tissue dehydration, labeling or staining. Together, this shows that polarized light microscopy is a powerful method for studying collagen architecture in the eye, with applications ranging from normal physiology and aging, to pathology and transplantation.

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“…Most studies focus on the uniaxial tendon properties aligned with the primary load direction. There are few studies that consider the biaxial properties of the tendon [10,11]. However, these studies consider one longitudinal and one transverse direction.…”

Section: Introductionmentioning

confidence: 99%

Biomechanical and Biochemical Study of Muscle-Tendon-Bone in Porcine Digital Flexor Tendon

Danley

Huang

2015

Volume 9: Mechanics of Solids, Structures and Fluids

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The musculoskeletal system provides the body with both movement and support. In particular, contractile forces developed in the muscles are transmitted through the muscle-tendon junction (MTJ) into the tendon and then through the tendon-bone insertion into the bone. Each junction occurs between two dissimilar materials (muscle-to-tendon and tendon-to-bone) and neither is fully understood. The current study analyzes the relationship between the tissue microstructure and macro-scale biomechanical properties of the muscle-tendon-bone unit to better understand the anisotropic mechanical behavior of the tissue. Collagen content was assayed at various locations along the porcine digital flexor tendon. Collagen concentration as a percent of the wet weight in the bone end was found to be 20.4±5.2% (n=6), the mid tendon to be 20.6±5.3% (n=6), and the muscle end to be 25.2±3.6% (n=4). No statistical differences were found between these collagen concentrations. Additionally, to the best of the authors’ knowledge, this is the first study to report cross-sectional stress-strain data for the muscle-tendon-bone unit. Results indicate that the tendon cross-sectional stiffness increases from the proximal end to the distal end. However, no direction dependent anisotropies were found between the mediolateral and dorsopalmar directions. Effects of microstructural components, such as glycosaminoglycans and collagen, and phenomenon such as fibril sliding and cross-linking, are discussed in relation to the reported cross-section stress-strain response. This work provides a synergistic approach for quantifying biomechanical and biochemical properties of biological tissue, and potentially facilitates the development of tissue engineered MTJ and tendon-bone insertion.

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Biaxial tensile testing and constitutive modeling of human supraspinatus tendon

Cited by 7 publications

References 39 publications

Biaxial mechanics and inter‐lamellar shearing of stem‐cell seeded electrospun angle‐ply laminates for annulus fibrosus tissue engineering

Biaxial mechanics and inter‐lamellar shearing of stem‐cell seeded electrospun angle‐ply laminates for annulus fibrosus tissue engineering

Polarization microscopy for characterizing fiber orientation of ocular tissues

Biomechanical and Biochemical Study of Muscle-Tendon-Bone in Porcine Digital Flexor Tendon

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