Transparent exopolymer particles (TEP), particulate organic carbon (POC), and particles (size range: 5.2–119 μm) as determined by laser in situ scattering and transmissometry (LISST) were measured in the water column from the Chukchi Sea to the Canada Basin in the western Arctic Ocean, during the late summer of 2012. In general, the percentages of TEP‐carbon to POC were high (the mean values for the shelf and slope‐basin regions were 135.4 ± 58.0% (± standard deviation, n = 36) and 187.6 ± 73.3% (n = 58), respectively), relative to the corresponding values reported for other oceanic regions, suggesting that TEP play an important role in regulating particle dynamics. A hotspot (extremely high concentration) of particles, accompanied by high prokaryote abundance and production, was observed near the seafloor (depth 50 m) of the shelf region. Localized accumulation of particles was also found in the thin layer near the pycnocline (depth 10–30 m) and on the slope. Over a broader spatial scale, particle concentration gradients were identified from the shelf to the basin in the upper water column (<50 m). The particle‐size distribution analysis indicated that relatively small particles were dominant in the shelf region compared to the slope‐basin region. These results suggest that particles containing large amounts of TEP are produced in the shelf region and are potentially delivered to the slope‐basin region along the pycnocline, which might support productivity and material cycles in the nutrient‐depleted basin region of the western Arctic Ocean.