Bicuculline increases the intracellular calcium response of CA1 hippocampal neurons to synaptic stimulation

Linden, J.A. van der; Joëls, Marian; Karst, H.; Juta, A.J.A.; Wadman, W.J.

doi:10.1016/0304-3940(93)90715-w

Cited by 19 publications

(6 citation statements)

References 24 publications

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“…Differentiation of NSCs, including formation of functional neural networks is sustained and very consistent in the 3D ES tissue models. Remarkably, the bicuculline‐induced amplification of firing rate and spike amplitude of cells in stimulated tissues shown by calcium imaging is consistent with enhanced maturation of neural networks, which include a GABAergic neuronal population …”

supporting

confidence: 62%

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Tomaskovic-Crook

Zhang

Ahtiainen

et al. 2019

Adv Healthcare Materials

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Electricity is important in the physiology and development of human tissues such as embryonic and fetal development, and tissue regeneration for wound healing. Accordingly, electrical stimulation (ES) is increasingly being applied to influence cell behavior and function for a biomimetic approach to in vitro cell culture and tissue engineering. Here, the application of conductive polymer (CP) poly(3,4‐ethylenedioxythiophene)‐polystyrenesulfonate (PEDOT:PSS) pillars is described, direct‐write printed in an array format, for 3D ES of maturing neural tissues that are derived from human neural stem cells (NSCs). NSCs are initially encapsulated within a conductive polysaccharide‐based biogel interfaced with the CP pillar microelectrode arrays (MEAs), followed by differentiation in situ to neurons and supporting neuroglia during stimulation. Electrochemical properties of the pillar electrodes and the biogel support their electrical performance. Remarkably, stimulated constructs are characterized by widespread tracts of high‐density mature neurons and enhanced maturation of functional neural networks. Formation of tissues using the 3D MEAs substantiates the platform for advanced clinically relevant neural tissue induction, with the system likely amendable to diverse cell types to create other neural and non‐neural tissues. The platform may be useful for both research and translation, including modeling tissue development, function and dysfunction, electroceuticals, drug screening, and regenerative medicine.

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supporting

confidence: 62%

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Tomaskovic-Crook

Zhang

Ahtiainen

et al. 2019

Adv Healthcare Materials

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“…Significant increases in [Ca 2+ ] i have been reported during epileptiform activity for various model systems (Sinha et al . 1995; van der Linden et al . 1993; Albowitz et al .…”

Section: Resultsmentioning

confidence: 99%

Free radical production correlates with cell death in an in vitro model of epilepsy

Frantseva

Velázquez

Hwang

et al. 2000

Eur J of Neuroscience

118

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Free radical (FR) production, a major step in calcium-dependent neurodegeneration, has been linked to the generation of epileptiform activity and seizure-induced cell death. However, direct evidence of FR production in neurons during seizures has never been presented. Using hippocampal cultured slices we demonstrate that FRs are produced in CA3 but not CA1 pyramidal neurons during the rhythmic synchronous activity induced by the GABAA receptor antagonist bicuculline. The production of FRs (measured as changes in the fluorescence emission of dihydrorhodamine 123) was correlated with an increase in the baseline levels of intracellular calcium ([Ca2+]i) estimated by fluo-3 injected into individual neurons via a patch pipette. [Ca2+]i increased during spike bursting and returned to baseline levels after the burst termination in CA1, but not in CA3, pyramidal neurons where 'interburst' calcium concentrations progressively increased. Measurement of cell death, performed with propidium iodide 48 h after a 30-min exposure to bicuculline, revealed most prominent degeneration of pyramidal neurons in the CA3 pyramidal layer. The FR scavengers vitamin E and glutathione significantly reduced the seizure-induced neurodegeneration without supressing spontaneous epileptiform activity. These observations indicate that FR overproduction is related to seizure-induced neuronal death.

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“…Previous studies (Cavus and Teyler 1996;Grover and Teyler 1990) indicated that induction of this NMDA receptor-independent LTP requires Ca 2ϩ influx through postsynaptic voltage-dependent Ca 2ϩ channels (VDCCs). During tetanization, GABA A receptor-mediated inhibition limits the degree of postsynaptic depolarization and postsynaptic action potential firing and thus limits the magnitude of Ca 2ϩ influx into postsynaptic neurons (van der Linden et al 1993). Relief from GABA A inhibition should therefore enhance postsynaptic Ca 2ϩ entry and thereby facilitate induction of LTP.…”

Section: Introductionmentioning

confidence: 99%

Blockade of GABA_A Receptors Facilitates Induction of NMDA Receptor-Independent Long-Term Potentiation

Grover

Chen

1999

Journal of Neurophysiology

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An N-methyl-D-aspartate (NMDA)-independent form of long-term potentiation (LTP), which depends on postsynaptic, voltage-dependent calcium channels (VDCCs), has been demonstrated in area CA1 of hippocampus. GABA acting at GABAA receptors limits postsynaptic depolarization during LTP induction. Blockade of GABAA receptors should therefore enhance activation of postsynaptic VDCCs and facilitate the induction of this NMDA receptor-independent, VDCC-dependent LTP. In agreement with this hypothesis, pharmacological blockade of GABAA receptors in the in vitro rat hippocampal slice increased the magnitude of LTP resulting from a normally effective, high-frequency (200 Hz) tetanic stimulation protocol. In addition, GABAA receptor blockade allowed a lower frequency (25 Hz) and normally ineffective tetanic stimulation protocol to induce this form of LTP. Intracellular recordings from CA1 pyramidal cells revealed that blocking GABAA receptors during tetanic stimulation allowed greater postsynaptic depolarization, increased the number of postsynaptic action potentials fired during the tetanization, and also increased the duration of synaptically evoked action potentials. To mimic the increased action potential firing observed when GABAA receptors were blocked, we paired 25-Hz antidromic stimulation with 25-Hz orthodromic stimulation. Paired antidromic + orthodromic 25-Hz stimulation induced NMDA receptor-independent LTP, whereas neither antidromic nor orthodromic stimulation alone induced LTP. Increased action potential firing can therefore at least partially account for the facilitation of NMDA receptor-independent LTP caused by blockade of GABAA receptors. This conclusion is consistent with prior studies demonstrating that action potentials are particularly effective stimuli for the gating of VDCCs in CA1 pyramidal cell dendrites.

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Bicuculline increases the intracellular calcium response of CA1 hippocampal neurons to synaptic stimulation

Cited by 19 publications

References 24 publications

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Free radical production correlates with cell death in an in vitro model of epilepsy

Blockade of GABA_A Receptors Facilitates Induction of NMDA Receptor-Independent Long-Term Potentiation

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Bicuculline increases the intracellular calcium response of CA1 hippocampal neurons to synaptic stimulation

Cited by 19 publications

References 24 publications

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Free radical production correlates with cell death in an in vitro model of epilepsy

Blockade of GABAA Receptors Facilitates Induction of NMDA Receptor-Independent Long-Term Potentiation

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Product

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Blockade of GABA_A Receptors Facilitates Induction of NMDA Receptor-Independent Long-Term Potentiation