Bicyclic peptide-based assay for uPA cancer biomarker

Moro, Giulia; Sfragano, Patrick Severin; Ghirardo, Jessica; Mazzocato, Ylenia; Angelini, Alessandro; Palchetti, Ilaria; Polo, Federico

doi:10.1016/j.bios.2022.114477

Cited by 7 publications

(20 citation statements)

References 24 publications

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“…They were employed as bioreceptors for h-uPA in an electrochemical assay. The results were very promising in terms of reproducibility, selectivity, and sensitivity with LODs of 105.8 ng mL −1 and 32.5 ng mL −1 for P 1 and P 2 , respectively, which are close to the nanomolar concentration range required for diagnostic purposes (see Table 1) [36]. Therefore, inspired by such positive results, a novel bicyclic peptide, P 3 , was designed and synthesized.…”

Section: Introductionmentioning

confidence: 84%

“…These findings drove the attention of researchers towards the possible integration of such bicyclic peptides as bioreceptors in sensing platforms for cancer diagnostics. Very recently, two biotinylated bicyclic peptides, namely P 1 and P 2 , were designed to this end [36]. P 1 was derived from UK18, whereas P 2 offered a slightly modified sequence to outperform P 1 .…”

Section: Introductionmentioning

confidence: 99%

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An Impedimetric Biosensing Strategy Based on BicyclicPeptides as Bioreceptors for Monitoring h-uPA Cancer Biomarkers

et al. 2023

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In the era of liquid biopsies, the reliable and cost-effective detection and screening of cancer biomarkers has become of fundamental importance, thus paving the way for the advancement of research in the field of point-of-care testing and the development of new methodologies and technologies. Indeed, the latter ones can help designing advanced diagnostic tools that can offer portability, ease of use with affordable production and operating costs. In this respect, impedance-based biosensing platforms might represent an attractive alternative. In this work, we describe a proof-of-concept study aimed at designing portable impedimetric biosensors for the monitoring of human urokinase-type plasminogen activator (h-uPA) cancer biomarker by employing small synthetic receptors. Aberrant levels of h-uPA were correlated with different types of cancers. Herein, we report the use of two bicyclic peptides (P2 and P3) which have been engineered to bind h-uPA with high affinity and exquisite specificity. The synthetic receptors were immobilized via biotin-streptavidin chemistry on the surface of commercial screen-printed electrodes. The impedimetric changes in the electrode/solution interface upon incubation of spiked h-uPA samples in the presence of a redox probe were followed via electrochemical impedance spectroscopy. The P3-based impedimetric assay showed the best outcomes in terms of dynamic range and linearity (0.01–1 μg mL−1) and sensitivity (LOD = 9 ng mL−1). To fully assess the performances of P3 over P2, and to compare the label-free architecture vs. labelled architecture, a voltammetric assay was also developed.

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Section: Introductionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

An Impedimetric Biosensing Strategy Based on BicyclicPeptides as Bioreceptors for Monitoring h-uPA Cancer Biomarkers

et al. 2023

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“…Meanwhile, peptides have significant advantages, such as good stability, ease of synthesis, and low-cost [32] . Consequently, they have been widely applied in biosensing, tumor therapy and templated synthesis [33] , [34] , [35] , [36] , [37] .…”

Section: Introductionmentioning

confidence: 99%

An ultrasensitive ELISA to assay femtomolar level SARS-CoV-2 antigen based on specific peptide and tyramine signal amplification

Liu

Pang

Wang

et al. 2023

Sensors and Actuators B: Chemical

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“…Furthermore, p -aminophenol (PAP) was also tested for its importance in many analytical applications and as a reaction product of alkaline phosphatase (AP) enzymatic hydrolysis of p -aminophenyl phosphate. AP is a widely used enzyme in optical and electrochemical biosensors as a label of an affinity reaction. − Herein, we investigate the possibility of using AP as a label of affinity reactions in PEC biosensing at a TiO 2 /ITO electrode decorated with the ruthenium(II) photosensitizer L.…”

Section: Introductionmentioning

confidence: 99%

“…AP is a widely used enzyme in optical and electrochemical biosensors as a label of an affinity reaction. 13 − 16 Herein, we investigate the possibility of using AP as a label of affinity reactions in PEC biosensing at a TiO 2 /ITO electrode decorated with the ruthenium(II) photosensitizer L.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Ruthenium(II) Complex in Singlet Oxygen-Mediated Photoelectrochemical Sensing

et al. 2022

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A water-soluble ruthenium(II) complex (L), capable of producing singlet oxygen (1O2) when irradiated with visible light, was used to modify the surface of an indium–tin oxide (ITO) electrode decorated with a nanostructured layer of TiO2 (TiO2/ITO). Singlet oxygen triggers the appearance of a cathodic photocurrent when the electrode is illuminated and biased at a proper reduction potential value. The L/TiO2/ITO electrode was first characterized with cyclic voltammetry, impedance spectroscopy, NMR, and Raman spectroscopy. The rate constant of singlet oxygen production was evaluated by spectrophotometric measurements. Taking advantage of the oxidative process initiated by 1O2, the analysis of phenolic compounds was accomplished. Particularly, the 1O2-driven oxidation of hydroquinone (HQ) produced quinone moieties, which could be reduced back at the electrode surface, biased at −0.3 V vs Ag/AgCl. Such a light-actuated redox cycle produced a photocurrent dependent on the concentration of HQ in solution, exhibiting a limit of detection (LOD) of 0.3 μmol dm–3. The L/TiO2/ITO platform was also evaluated for the analysis of p-aminophenol, a commonly used reagent in affinity sensing based on alkaline phosphatase.

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Bicyclic peptide-based assay for uPA cancer biomarker

Cited by 7 publications

References 24 publications

An Impedimetric Biosensing Strategy Based on BicyclicPeptides as Bioreceptors for Monitoring h-uPA Cancer Biomarkers

An Impedimetric Biosensing Strategy Based on BicyclicPeptides as Bioreceptors for Monitoring h-uPA Cancer Biomarkers

An ultrasensitive ELISA to assay femtomolar level SARS-CoV-2 antigen based on specific peptide and tyramine signal amplification

Characterization of a Ruthenium(II) Complex in Singlet Oxygen-Mediated Photoelectrochemical Sensing

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