This paper is successfully demonstrated simulative comparison and investigation of 32 channels with 0.8 nm frequency spacing bidirectional wavelength division multiplexing passive optical network (WDM-PON) having 640 and 320 GB/s data rate in downstream and upstream transmission by using CRZ-DPSK/NRZ modulation formats respectively. The maximum reach of the system has been configured to 100 km by employing different post-dispersion compensation techniques such as EDC, DCF, OPC, and FBG in uplink and downlink transmission for compensate the effect of chromatic dispersion in propagating channel. An EDFA optical amplifier is placed after DCF for boosting the amplitude of weak signals downstream. Apart from this, the RSOA amplifier is installed at the ONT end for reflecting and re-modulates the same wavelengths to the CO terminal without using any external optical light source. The performance comparison of distinct compensation schemes are evaluated in terms of BER, Q factor, and Eye diagrams. It has been observed that the FBG and DCF are more robust compensation techniques as compared to EDC and OPC in the bidirectional WDM-PON. The maximum satisfying value of BER has been observed in downlink and uplink transmissions are 10−26 and 10−13 for FBG and DCF respectively.