Bidirectional emission of organic compounds by decaying leaf litter of a number of forest-forming tree species in the northern hemisphere

Isidorov, Valery; Maslowiecka, Jolanta; Sarapultseva, Polina

doi:10.1016/j.geoderma.2024.116812

Cited by 2 publications

(2 citation statements)

References 66 publications

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“…Leaf litter samples were collected four times: at the beginning of decomposition (after leaf fall, 0 months) and after 6, 15, and 18 months of decomposition. A detailed description of the field experiment and photos of sampling sites and litter were given in our previous study [15]. At the onset of our experiments, freshly fallen leaves from three distinct tree-forming plant species were collected into sterile Petri dishes (two plates at each litter type).…”

Section: Study Area Experiments Design and Litter Decompositionmentioning

confidence: 99%

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Unraveling the Role of Bacteria in Nitrogen Cycling: Insights from Leaf Litter Decomposition in the Knyszyn Forest

Khomutovska,

Jasser,

Isidorov

2024

Forests

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Microorganisms are vital in leaf litter decomposition and contribute significantly to global nutrient cycling. However, there is a need for improved understanding of the taxonomic and functional diversity of litter-associated bacteria. The Knyszyn Forest comprises a unique ecosystem providing diverse microhabitats for microorganisms in central Europe, similar to the southwestern taiga in many respects. This study presents the results of high-throughput sequencing performed for Betula pendula, B. pubescens, and Carpinus betulus litter-associated microbial communities from northern Poland. Microbial assemblage composition and structure at different stages of litter decomposition revealed the domination of phyllosphere-associated taxa of Sphingomonas and Pseudomonas in bacterial communities in the early stages. Meanwhile, at the later stages of decomposition, the representation of soil-associated bacterial communities, such as Pedobacter, was higher. This study identifies key bacteria (Pedobacter, Mucilaginibacter, and Luteibacter) as pivotal in nutrient cycling through cellulose and hemicellulose decomposition, dominating later decomposition phases. Taxonomic analysis based on functional markers associated with nitrogen metabolism highlights the pivotal role of specific Pseudomonadota (Proteobacteria) taxa in driving nitrogen cycling dynamics during litter decomposition. Most of these taxa were unclassified at the genus level, particularly in the later stages of litter decomposition, and are crucial in mediating nitrogen transformation processes, underscoring their significance in ecosystem nutrient cycling.

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Section: Study Area Experiments Design and Litter Decompositionmentioning

confidence: 99%

“…DNA extraction utilized the E.Z.N.A. ® Soil DNA Kit (Omega Bio-Tek, Norcross, GA, USA), with optimization based on previous work [15]. DNA extraction was performed 5-10 times per sample, depending on DNA amount and quality.…”

Section: Dna Extraction Sequencing and Bioinformaticsmentioning

confidence: 99%

Unraveling the Role of Bacteria in Nitrogen Cycling: Insights from Leaf Litter Decomposition in the Knyszyn Forest

Khomutovska,

Jasser,

Isidorov

2024

Forests

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Seasonal dynamics in leaf litter decomposing microbial communities in temperate forests: a whole-genome- sequencing-based study

Khomutovska,

Jasser,

Sarapultseva

et al. 2024

PeerJ

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Leaf litter decomposition, a crucial component of the global carbon cycle, relies on the pivotal role played by microorganisms. However, despite their ecological importance, leaf-litter-decomposing microorganism taxonomic and functional diversity needs additional study. This study explores the taxonomic composition, dynamics, and functional role of microbial communities that decompose leaf litter of forest-forming tree species in two ecologically unique regions of Europe. Twenty-nine microbial metagenomes isolated from the leaf litter of eight forest-forming species of woody plants were investigated by Illumina technology using read- and assembly-based approaches of sequences analysis. The taxonomic structure of the microbial community varies depending on the stage of litter decomposition; however, the community’s core is formed by Pseudomonas, Sphingomonas, Stenotrophomonas, and Pedobacter genera of Bacteria and by Aureobasidium, Penicillium, Venturia genera of Fungi. A comparative analysis of the taxonomic structure and composition of the microbial communities revealed that in both regions, seasonal changes in structure take place; however, there is no clear pattern in its dynamics. Functional gene analysis of MAGs revealed numerous metabolic profiles associated with leaf litter degradation. This highlights the diverse metabolic capabilities of microbial communities and their implications for ecosystem processes, including the production of volatile organic compounds (VOCs) during organic matter decomposition. This study provides important advances in understanding of ecosystem processes and the carbon cycle, underscoring the need to unravel the intricacies of microbial communities within these contexts.

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Bidirectional emission of organic compounds by decaying leaf litter of a number of forest-forming tree species in the northern hemisphere

Cited by 2 publications

References 66 publications

Unraveling the Role of Bacteria in Nitrogen Cycling: Insights from Leaf Litter Decomposition in the Knyszyn Forest

Unraveling the Role of Bacteria in Nitrogen Cycling: Insights from Leaf Litter Decomposition in the Knyszyn Forest

Seasonal dynamics in leaf litter decomposing microbial communities in temperate forests: a whole-genome- sequencing-based study

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