BACKGROUND Bidirectional ventricular tachycardia (BD-VT) is an intriguing arrhythmia, characterized by a beat-to-beat alternation of the QRS polarity on electrocardiogram. Currently there is no simple BD-VT animal model. OBJECTIVE We report a simple animal model of BD-VT induced by caffeine and dobutamine (C1D) challenge in normal rats in which the arrhythmia can be attenuated by dantrolene (a ryanodine receptor stabilizer) treatment, but not by the pacemaker channel blocker ivabradine treatment. METHODS Adult (4-5 months old) Sprague-Dawley rats (both sexes) were randomized into C1D (n 5 8, received caffeine 120 mg/kg intraperitoneally [IP] and dobutamine 60 mg/kg IP, sequentially) and control (n 5 8) groups. In addition, a group of 7 rats were pretreated with dantrolene (10 mg/kg, IP) 30 minutes before the C1D challenge and another group of 8 rats were pretreated with ivabradine (5 mg/kg, IP) 30 minutes before the C1D challenge. RESULTS C1D challenge induced spontaneous premature ventricular contractions (PVCs) in 7 of 8 rats and BD-VT (lasted 4.3 6 2.9 minutes, terminated spontaneously) in 6 of 8 (75%) rats. No ventricular arrhythmia was induced in the control group (P , .05 vs C1D group). Dantrolene treatment significantly decreased BD-VT (1 of 7 rats in the Dantrolene1C1D group vs 6 of 8 rats in C1D group, P , .05). Ivabradine treatment did not affect C1D-induced BD-VT (7 of 8 rats in the Ivabradine1C1D group vs 6 of 8 in the C1D group, P. .05). CONCLUSION Caffeine and dobutamine challenge induces BD-VT in a majority of normal rats. Stabilizing cardiac ryanodine receptors with dantrolene treatment can significantly decrease the occurrence of BD-VT, but pacemaker channel blocker ivabradine treatment does not have effect in this animal model.