Bidirectional Zn-Fluxes and Compartmentation in Wheat Seedling Roots

Maria, Guillermo Esteban Santa; Cogliatti, D. H.

doi:10.1016/s0176-1617(88)80112-3

Cited by 53 publications

(8 citation statements)

References 18 publications

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“…In contrast to manganese, zinc is normally present in plants at high concentrations. For example, in roots of wheat seedlings, the cytoplasmic concentration of total Zn has been estimated to be approximately 0.4 m M (Santa Maria and Cogliatti 1988). According to Cakmak (2000), Zn‐deficiency‐related disturbances in cellular metabolism often resulting in inhibition of plant growth, are due to oxidative damage at membrane proteins, phospholipids, chlorophyll, DNA, SH‐containing enzymes and indole‐3‐acetic acid.…”

Section: Discussionmentioning

confidence: 99%

“…Zinc is normally present in plants at high concentrations (Santa Maria and Cogliatti 1988) and its deficiency is one of the most widespread micronutrient deficiencies in plants, causing severe reductions in crop production (Cakmak 2000). Increasing studies indicate that oxidative damage to cellular components caused in plants by ROS results from a deficiency of zinc (Pinton et al.…”

Section: Introductionmentioning

confidence: 99%

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Retardation and inhibition of the cation‐induced superoxide generation in BY‐2 tobacco cell suspension culture by Zn²⁺ and Mn²⁺

Kawano

Muto

et al. 2002

Physiologia Plantarum

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Salts at high concentrations may cause oxidative damage to plant cells since many studies indicated the involvement of reactive oxygen species in salt-stress response. Recently, we have demonstrated that treatment of tobacco (Nicotiana tabacum) cell suspension culture with various salts result in an immediate burst of superoxide production via activation of NADPH oxidase by ions of alkali metals (Li+, Na+, K+), alkali earth metals (Mg2+, Ca2+) or lanthanides (La3+, Gd3+). In this study, we tested the effect of extracellular supplementation of Zn2+ and Mn2+ on the cation-induced oxidative burst in tobacco cell suspension culture, measured with a superoxide-specific Cypridina luciferin-derived chemiluminescent reagent. Extracellular supplementation of Zn2+ and Mn2+ inhibited the generation of superoxide in response to addition of salts. Although both Zn2+ and Mn2+ inhibited the salt-induced generation of superoxide, the modes of inhibition by those ions seemed to be different since Mn2+ simply inhibited total production of superoxide while Zn2+ inhibited the early phase of superoxide production and induced the slow release of superoxide. Roles of Mn2+ and Zn2+ in protection of plant cells from salt stress, as an effective superoxide scavenger and an effective inhibitor of plasma membrane-bound NADPH oxidase, respectively, are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Retardation and inhibition of the cation‐induced superoxide generation in BY‐2 tobacco cell suspension culture by Zn²⁺ and Mn²⁺

Kawano

Muto

et al. 2002

Physiologia Plantarum

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show abstract

“…Zinc is normally present in plants at high concentrations ( Santa-Maria and Cogliatti, 1988 ) and its deficiency is one of the most widespread micronutrient deficiencies in plants, causing severe reductions in crop production ( Cakmak, 2000 ). Increasing studies indicate that oxidative damage to cellular components caused in plants being challenged by ROS, is highly due to the deficiency of zinc ( Pinton et al, 1994 ; Cakmak, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Zinc-Dependent Protection of Tobacco and Rice Cells From Aluminum-Induced Superoxide-Mediated Cytotoxicity

et al. 2015

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Al3+ toxicity in growing plants is considered as one of the major factors limiting the production of crops on acidic soils worldwide. In the last 15 years, it has been proposed that Al3+ toxicity are mediated with distortion of the cellular signaling mechanisms such as calcium signaling pathways, and production of cytotoxic reactive oxygen species (ROS) causing oxidative damages. On the other hand, zinc is normally present in plants at high concentrations and its deficiency is one of the most widespread micronutrient deficiencies in plants. Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells. Previously, inhibitory action of Zn2+ against lanthanide-induced superoxide generation in tobacco cells have been reported, suggesting that Zn2+ interferes with the cation-induced ROS production via stimulation of NADPH oxidase. In the present study, the effect of Zn2+ on Al3+-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv. Nipponbare), was examined. The Zn2+-dependent inhibition of the Al3+-induced oxidative burst was observed in both model cells selected from the monocots and dicots (rice and tobacco), suggesting that this phenomenon (Al3+/Zn2+ interaction) can be preserved in higher plants. Subsequently induced cell death in tobacco cells was analyzed by lethal cell staining with Evans blue. Obtained results indicated that presence of Zn2+ at physiological concentrations can protect the cells by preventing the Al3+-induced superoxide generation and cell death. Furthermore, the regulation of the Ca2+ signaling, i.e., change in the cytosolic Ca2+ ion concentration, and the cross-talks among the elements which participate in the pathway were further explored.

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“…This approach has been a widely used technique to approximate plant subcellular ion fluxes, membrane kinetic parameters, and ionic concentrations within subcellular compartments. Santa Maria & Cogliatti (1988) found that 65 Zn efflux in wheat roots could be fitted to a triple exponential function and c. 8% of the root Zn was hypothesized to be in the cytoplasm, 76% in the vacuole, with the rest in the cell wall. When this approach was applied to leaf tissue from Zn‐efficient and ‐inefficient wheat cultivars grown under low Zn conditions, leaf compartmentation studies showed that both the efficient and inefficient genotypes had similar Zn content for the vacuole (83–85%) and cytoplasm (9–11%) (Hacisalihoglu et al ., 2003a).…”

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confidence: 99%

How do some plants tolerate low levels of soil zinc? Mechanisms of zinc efficiency in crop plants

2003

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Summary Researchers are beginning to understand how some plant genotypes can maintain reasonable growth and yields under low soil Zn, a trait termed zinc efficiency (ZE). Several studies have shown no correlation between ZE and root Zn uptake, Zn translocation to shoot, or shoot Zn accumulation. Furthermore, it has not been possible to conclusively link differences in leaf subcellular Zn compartmentation with ZE. However, biochemical Zn utilization, including the ability to maintain the activity of Zn requiring enzymes in response to Zn deficiency may be a key component of ZE. The next logical step in investigations of this trait is research on the genetic and molecular basis for ZE, in order to better understand the relationship between Zn utilization and ZE, and to identify the gene(s) controlling ZE. Progress in this research area could provide the knowledge to facilitate the engineering of Zn‐efficient plant varieties, which could help both crop production on marginal soils as well as possibly improve the micronutrient density of food crops to help address significant human nutrition problems related to micronutrient deficiency.

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Bidirectional Zn-Fluxes and Compartmentation in Wheat Seedling Roots

Cited by 53 publications

References 18 publications

Retardation and inhibition of the cation‐induced superoxide generation in BY‐2 tobacco cell suspension culture by Zn²⁺ and Mn²⁺

Retardation and inhibition of the cation‐induced superoxide generation in BY‐2 tobacco cell suspension culture by Zn²⁺ and Mn²⁺

Zinc-Dependent Protection of Tobacco and Rice Cells From Aluminum-Induced Superoxide-Mediated Cytotoxicity

How do some plants tolerate low levels of soil zinc? Mechanisms of zinc efficiency in crop plants

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Bidirectional Zn-Fluxes and Compartmentation in Wheat Seedling Roots

Cited by 53 publications

References 18 publications

Retardation and inhibition of the cation‐induced superoxide generation in BY‐2 tobacco cell suspension culture by Zn2+ and Mn2+

Retardation and inhibition of the cation‐induced superoxide generation in BY‐2 tobacco cell suspension culture by Zn2+ and Mn2+

Zinc-Dependent Protection of Tobacco and Rice Cells From Aluminum-Induced Superoxide-Mediated Cytotoxicity

How do some plants tolerate low levels of soil zinc? Mechanisms of zinc efficiency in crop plants

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Product

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Retardation and inhibition of the cation‐induced superoxide generation in BY‐2 tobacco cell suspension culture by Zn²⁺ and Mn²⁺

Retardation and inhibition of the cation‐induced superoxide generation in BY‐2 tobacco cell suspension culture by Zn²⁺ and Mn²⁺