Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways

James, Kieran; Motherway, Mary O’Connell; Bottacini, Francesca; Sinderen, Douwe van

doi:10.1038/srep38560

Cited by 132 publications

(170 citation statements)

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“…Newer studies examined the ability of select Bifidobacterium breve strains to utilize HMOs (Ruiz-Moyano et al 2013, James et al 2016). Comparative genomics of B. breve shows that this species contains clusters related to HMO utilization, including those related to sialic acid, fucose, and LNB (Bottacini et al 2014).…”

Section: Molecular Mechanisms Of Utilization Of Human Milk Oligosacchmentioning

confidence: 99%

“…B. breve exhibits strain-specific growth on HMOs; however, most strains grow well on LNT (Ruiz-Moyano et al 2013). James et al (2016) used transcriptome analysis and functional studies to examine pathways involved in LNT and LNnT utilization by B. breve UCC 2003. In this work, galactosidases and solute-binding proteins from B. breve UCC 2003 associated with type I and II HMO utilization were characterized, and this strain used separate pathways that have overlapping properties to metabolize LNT and LNnT (James et al 2016).…”

Section: Molecular Mechanisms Of Utilization Of Human Milk Oligosacchmentioning

confidence: 99%

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Milk Glycans and Their Interaction with the Infant-Gut Microbiota

Kirmiz

Robinson

Shah

et al. 2018

Annu. Rev. Food Sci. Technol.

106

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Human milk is a unique and complex fluid that provides infant nutrition and delivers an array of bioactive molecules that serve various functions. Glycans, abundant in milk, can be found as free oligosaccharides or as glycoconjugates. Milk glycans are increasingly linked to beneficial outcomes in neonates through protection from pathogens and modulation of the immune system. Indeed, these glycans influence the development of the infant and the infant-gut microbiota. Bifidobacterium species commonly are enriched in breastfed infants and are among a limited group of bacteria that readily consume human milk oligosaccharides (HMOs) and milk glycoconjugates. Given the importance of bifidobacteria in infant health, numerous studies have examined the molecular mechanisms they employ to consume HMOs and milk glycans, thus providing insight into this unique enrichment and shedding light on a range of translational opportunities to benefit at-risk infants.

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Section: Molecular Mechanisms Of Utilization Of Human Milk Oligosacchmentioning

confidence: 99%

Section: Molecular Mechanisms Of Utilization Of Human Milk Oligosacchmentioning

confidence: 99%

Milk Glycans and Their Interaction with the Infant-Gut Microbiota

Kirmiz

Robinson

Shah

et al. 2018

Annu. Rev. Food Sci. Technol.

106

View full text Add to dashboard Cite

show abstract

“…A more recent study by James et al [26] showed that recombinant lntA protein of B. breve UCC2003 [a homologue of GL-BG (IntA, as illustrated in Fig. 5)] showed hydrolytic activity toward lacto-N-tetoraose (Galb1-3GlcNAcb1-3Galb1-4Glc), degrading it into galactose and triose Lacto-N-triose [26]. Therefore, we also investigated the b-galactosidase activity of GL-SBP1 from B. breve YIT 4014 T against LNT, LNB, lactose, 3¢-GL, 4¢-GL and 6¢-GL (Fig.…”

Section: Discussionmentioning

confidence: 96%

“…Our data agreed well with these notable publications; furthermore, we found that these genes were involved in 3¢-and 6¢-GL utilization, but not 4¢-GL utilization. A more recent study by James et al [26] showed that recombinant lntA protein of B. breve UCC2003 [a homologue of GL-BG (IntA, as illustrated in Fig. 5)] showed hydrolytic activity toward lacto-N-tetoraose (Galb1-3GlcNAcb1-3Galb1-4Glc), degrading it into galactose and triose Lacto-N-triose [26].…”

Section: Discussionmentioning

confidence: 99%

Identification of genes involved in galactooligosaccharide utilization in Bifidobacterium breve strain YIT 4014T

et al. 2017

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Galactooligosaccharides (GOS) are mixed oligosaccharides that are mainly composed of galactosyllactoses (GLs), which include 3¢-GL, 4¢-GL, and 6¢-GL. Data from numerous in vitro and in vivo studies have shown that GOS selectively stimulate the growth of bifidobacteria. Previously, we identified the gene locus responsible for 4¢-GL utilization, but the selective routes of uptake and catabolism of 3¢-and 6¢-GL remain to be elucidated. In this study, we used differential transcriptomics to identify the utilization pathways of these GLs within the Bifidobacterium breve YIT 4014 T strain. We found that the BBBR_RS 2305-2320 gene locus, which includes a solute-binding protein (SBP) of an ATP-binding cassette (ABC) transporter and bgalactosidase, were up-regulated during 3¢-and 6¢-GL utilization. The substrate specificities of these proteins were further investigated, revealing that b-galactosidase hydrolyzed both 3¢-GL and 6¢-GL efficiently. Our surface plasmon resonance results indicated that the SBP bound strongly to 6¢-GL, but bound less tightly to 3¢-GL. Therefore, we looked for the other SBPs for 3¢-GL and found that the BBBR_RS08090 SBP may participate in 3¢-GL transportation. We also investigated the distribution of these genes in 17 bifidobacterial strains, including 9 B. breve strains, and found that the b-galactosidase genes were present in most bifidobacteria. Homologues of two ABC transporter SBP genes were found in all B. breve strains and in some bifidobacteria that are commonly present in the human gut microbiota. These results provide insights into the ability of human-resident bifidobacteria to utilize the main component of GOS in the gastrointestinal tract.

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“…Despite being the third most abundant solid component in breast milk after lactose (Lac) and lipids, HMOs have no nutritional value for infants because of their resistance to pancreatic digestion (Kunz et al, 2000;Urashima et al, 2012). Several groups, including our own, have found the gene sets coding for enzymes that degrade HMOs (Sela et al, 2008;Garrido et al, 2016;James et al, 2016;Katayama, 2016;Matsuki et al, 2016) and have shown that these genes are limited to the infant gut-associated bifidobacterial species among gut microbes (Ruiz-Moyano et al, 2013;Katayama, 2016;Thomson et al, 2017). These findings suggest that it is highly likely that HMOs serve as selective nutrients for bifidobacterial species.…”

Section: Introductionmentioning

confidence: 99%

Minority species influences microbiota formation: the role of Bifidobacterium with extracellular glycosidases in bifidus flora formation in breastfed infant guts

Gotoh

Ojima

Katayama

2019

Microbial Biotechnology

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Summary The human body houses a variety of microbial ecosystems, such as the microbiotas on the skin, in the oral cavity and in the digestive tract. The gut microbiota is one such ecosystem that contains trillions of bacteria, and it is well established that it can significantly influence host health and diseases. With the advancement in bioinformatics tools, numerous comparative studies based on 16S ribosomal RNA ( rRNA ) gene sequences, metabolomics, pathological and epidemical analyses have revealed the correlative relationship between the abundance of certain taxa and disease states or amount of certain causative bioactive compounds. However, the 16S rRNA ‐based taxonomic analyses using next‐generation sequencing ( NGS ) technology essentially detect only the majority species. Although the entire gut microbiome consists of 10 13 microbial cells, NGS read counts are given in multiples of 10 6 , making it difficult to determine the diversity of the entire microbiota. Some recent studies have reported instances where certain minority species play a critical role in creating locally stable conditions for other species by stabilizing the fundamental microbiota, despite their low abundance. These minority species act as ‘keystone species’, which is a species whose effect on the community is disproportionately large compared to its relative abundance. One of the attributes of keystone species within the gut microbiota is its extensive enzymatic capacity for substrates that are rare or difficult to degrade for other species, such as dietary fibres or host‐derived complex glycans, like human milk oligosaccharides ( HMO s). In this paper, we propose that more emphasis should be placed on minority taxa and their possible role as keystone species in gut microbiota studies by referring to our recent studies on HMO ‐mediated microbiota formation in the infant gut.

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Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways

Cited by 132 publications

References 78 publications

Milk Glycans and Their Interaction with the Infant-Gut Microbiota

Milk Glycans and Their Interaction with the Infant-Gut Microbiota

Identification of genes involved in galactooligosaccharide utilization in Bifidobacterium breve strain YIT 4014T

Minority species influences microbiota formation: the role of Bifidobacterium with extracellular glycosidases in bifidus flora formation in breastfed infant guts

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