Bifunctional alcohol/aldehyde dehydrogenase AdhE controls phospho-transferase system sugar utilization and virulence gene expression by interacting PtsH in Edwardsiella piscicida

Mao, Qiaoqiao; Jiang, Jihao; Wu, Xiao; Ma, Yue; Zhang, Yuanxing; Zhao, Yanni; Zhang, Yibei; Wang, Qiyao

doi:10.1016/j.micres.2022.127018

Cited by 2 publications

(2 citation statements)

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“…The underlying cause for this reduction is not clear, but may attribute to high NADH accumulation caused by suppression of ethanol production and/or other physiological roles of the enzymes encoded by the adh2 / 6 genes. It has been reported that a mutant strain of the bifunctional adhE exhibits transcriptional changes in flagellar and type III secretion system (T3SS), resulting in decreased virulence, mobility and colonization (Beckham et al, 2014; Johnston et al, 2019; Mao et al, 2022). Notably, the deletion of Φ3 in LR10 resulted in an abrupt increase in transformation efficiency by approximately 200‐fold (Figure 7a).…”

Section: Resultsmentioning

confidence: 99%

“…genes. It has been reported that a mutant strain of the bifunctional adhE exhibits transcriptional changes in flagellar and type III secretion system (T3SS), resulting in decreased virulence, mobility and colonization (Beckham et al, 2014;Johnston et al, 2019;Mao et al, 2022). Notably, the deletion of Φ3 in LR10 resulted in an abrupt increase in transformation efficiency by approximately 200fold (Figure 7a).…”

Section: Effect Of Rm Systems On Transformation Efficiencymentioning

confidence: 96%

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Construction of prophage‐free and highly‐transformable Limosilactobacillus reuteri strains and their use for production of 1,3‐propanediol

Singh,

Park

2023

Biotech & Bioengineering

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The lactic acid bacterium Limosilactobacillus reuteri (formerly Lactobacillus reuteri) is a desirable host for the production of 1,3‐propanediol (1,3‐PDO) from glycerol when 1,3‐PDO is used in the food or cosmetic industry. However, the production is hindered by strain instability, causing cell lysis, and difficult gene manipulation. This study reveals that the stability of L. reuteri DSM 20016 and its 1,3‐PDO production, especially in the alcohol dehydrogenases (ADHs)‐deletion mutants, are greatly enhanced after the deletion of two prophages (Φ3 and Φ4) present in the L. reuteri's chromosome. The resulting phage‐free and ADHs‐deletion mutant could produce >825 mM 1,3‐PDO in 48 h without cell lysis at the theoretical maximum yield on glucose of ~2 mol/mol. Compared to the wild‐type strain, the mutant exhibited a 45.2% increase in 1,3‐PDO production titer and a 2.1‐fold increase in yield. In addition, this study reports that the transformation efficiency of L. reuteri Δadh2Δadh6 mutant strains were greatly enhanced by >300‐fold after the deletion of prophage Φ3, probably due to the removal of a restriction‐modification (RM) system which resides in the phage genome. With improved stability and higher transformation efficiency, recombinant L. reuteri DSM 20016 Δadh2Δadh6ΔΦ3ΔΦ4 can be a more reliable and amenable host for industrial applications.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Rm Systems On Transformation Efficiencymentioning

confidence: 96%