Bifunctional plasmonic-magnetic particles for an enhanced microfluidic SERS immunoassay

Yap, Lim Wei; Chen, Huaying; Gao, Yuan; Petkovic, Karolina; Liang, Yan; Jye, Kae; Wang, Huanting; Tang, Zhiyong; Zhu, Yonggang; Cheng, Wenlong

doi:10.1039/c7nr01511a

Cited by 59 publications

(37 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a further example, Yap et al, reported on the design and use of a microfluidic device with bifunctional (SERS and magnetic) particles for immunoglobulin G (IgG) detection, as shown in Figure 3 A. It consists of a micromixer and an assay chamber able to perform multiple step assays for direct SERS detection on a microfluidic chip [ 68 ]. On-chip micro-mixing is performed with the help of four electromagnets controlled by a microcontroller unit.…”

Section: Sers and Microfluidics For Label-free Detectionmentioning

confidence: 99%

“… ( A ) Schematic illustration of the SERS-based immunoassay in a microfluidic chip for rabbit IgG detection (reprinted with permission from Royal Society of Chemistry [ 68 ]); ( B ) scheme depicting the working mechanism of the designed molecular beacon (top left) and the SERS spectra of the molecular beacon (MB) probes with different concentrations of target DNAs, (reprinted with permission from IOP Sciences [ 71 ]); ( C ) a schematic illustration of a SERS substrate with AuNPs is prepared using an APTMS-assisted surface-assembly method, then a polydimethylsiloxane (PDMS) stamp is brought in to make contact with the AuNPs layer, and the removal of the PDMS stamp lifts off the AuNPs and leaves the desired SERS-active pattern. SERS detection at the SERS-active substrate inside a microfluidic channel where SERS detection of ochratoxin A (OTA) using OTA aptamer took place (reprinted with permission from Royal Society of Chemistry [ 72 ]).…”

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

Surface-Enhanced Raman Scattering Spectroscopy and Microfluidics: Towards Ultrasensitive Label-Free Sensing

Kant

Abalde‐Cela

2018

Biosensors

View full text Add to dashboard Cite

Raman scattering and surface-enhanced Raman scattering (SERS) spectroscopy have demonstrated their potential as ultrasensitive detection techniques in the past decades. Specifically, and as a result of the flourishing of nanotechnology, SERS is nowadays one of the most powerful sensing techniques, not only because of the low detection limits that it can achieve, but also for the structural information that it offers and its capability of multiplexing. Similarly, microfluidics technology is having an increased presence not only in fundamental research, but also in the industry. The latter is because of the intrinsic characteristics of microfluidics, being automation, high-throughput, and miniaturization. However, despite miniaturization being an advantage, it comes together with the need to use ultrasensitive techniques for the interrogation of events happening in extremely small volumes. The combination of SERS with microfluidics can overcome bottlenecks present in both technologies. As a consequence, the integration of Raman and SERS in microfluidics is being investigated for the label-free biosensing of relevant research challenges.

show abstract

Section: Sers and Microfluidics For Label-free Detectionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Surface-Enhanced Raman Scattering Spectroscopy and Microfluidics: Towards Ultrasensitive Label-Free Sensing

Kant

Abalde‐Cela

2018

Biosensors

View full text Add to dashboard Cite

show abstract

“…Examples of microfluidic devices coupled with Raman spectroscopy have been successfully used for sample preconcentration or storing cells during the spectroscopic analysis [22][23][24].…”

Section: Advances In Condensed Matter Physicsmentioning

confidence: 99%

Single File Flow of Biomimetic Beads for Continuous SERS Recording in a Microfluidic Device

Calzavara

Ferraro

Litti

et al. 2018

Advances in Condensed Matter Physics

View full text Add to dashboard Cite

A major challenge in cancer treatment is the quantification of biomarkers associated with a specific cancer type. Important biomarkers are the circulating tumor cells (CTCs) detached from the main cancer and circulating in the blood. CTCs are very rare and their identification is still an issue. Although CTCs quantification can be estimated by using fluorescent markers, all the fluorescence techniques are strongly limited by the number of emissions (therefore markers) that can be discriminated with one exciting line, by their bleaching characteristics, and by the intrinsic autofluorescence of biological samples. An emerging technique that can overcome these limitations is Surface Enhanced Raman Scattering (SERS). Signals of vibrational origin with intensity similar to those of fluorescence, but narrower bandwidths, can be easily discriminated even by exciting with a single laser line. We recently showed the benefit of this method with cells fixed on a surface. However, this approach is too demanding to be applied in clinical routine. To effectively increase the throughput of the SERS analysis, microfluidics represents a promising tool. We report two different hydrodynamic strategies, based on device geometry and liquids viscosity, to successfully combine a microfluidic design with SERS.

show abstract

“…Due to the resonance absorption of surface plasmons, SERS overcomes the shortcomings of the low sensitivity of Raman spectroscopy [4]. And SERS can obtain molecular structure information that is difficult to obtain by NRS [5, 6, 7]. Therefore, this photoinduced catalytic reaction also brings many applications [8].…”

Section: Introductionmentioning

confidence: 99%

Solvent-controlled plasmon-assisted surface catalysis reaction of 4-aminothiophenol dimerizing to p,p'-dimercaptoazobenzene on Ag nanoparticles

Liu

Yang

Zhao

et al. 2019

Heliyon

View full text Add to dashboard Cite

A large number of literatures have investigated the selective photocatalytic reaction of 4-aminothiophenol (PATP) to p,p'-dimercaptoazobenzene (DMAB). Most of them mainly study the contribution of substrate, excitation wavelength, exposure time, pH and added cations to plasmon-assisted surface catalytic reactions. However, we mainly study focuses on the effects of solvents on the dimerization of PATP to DMAB under the action of Ag nanoparticles (NPs). In experiments, a variety of diols was selected as solvents for the probe molecule PATP, and power-dependent SERS spectra were obtained at an excitation wavelength of 532 nm. From the laser-dependent SERS spectrum, we found that the characteristic peak enhancement effect of the product DMAB in different solvents is significantly different. That is, different solvents could regulate the rate at which DMAB is produced from PATP. Based on the experimental results, we further explored how different diol solvents regulate the response of PATP to DMAB. Our conclusion is that the solvent in the system can quickly capture the hot electrons generated by the decay of the plasmon, so that the remaining holes can oxidize PATP to form DMAB. The ability to trap hot electrons is different due to the difference in the position of the functional groups in the solvent, so that the photocatalytic reaction rate of the hole-oxidized PATP is different. The ability to capture electrons varies depending on the position of the functional groups in the solvent, so the oxidation rate of the photocatalytic reaction is also different. This work not only deepens our understanding of the mechanism of hole-driven surface catalysis oxidation reaction, but also provides a convenient method for regulating the rate of catalytic oxidation.

show abstract

Bifunctional plasmonic-magnetic particles for an enhanced microfluidic SERS immunoassay

Cited by 59 publications

References 31 publications

Surface-Enhanced Raman Scattering Spectroscopy and Microfluidics: Towards Ultrasensitive Label-Free Sensing

Surface-Enhanced Raman Scattering Spectroscopy and Microfluidics: Towards Ultrasensitive Label-Free Sensing

Single File Flow of Biomimetic Beads for Continuous SERS Recording in a Microfluidic Device

Solvent-controlled plasmon-assisted surface catalysis reaction of 4-aminothiophenol dimerizing to p,p'-dimercaptoazobenzene on Ag nanoparticles

Contact Info

Product

Resources

About