Bifunctional Protein TC1 Mediated One‐Pot Strategy for Robust Immobilization of DNA with High Accessibility

Abstract

Immobilizing DNA with high accessibility at the interface is attractive but challenging. Current methods often involve multiple chemical reactions and derivatives. In this study, an endonuclease, TC1, is introduced to develop a robust strategy for immobilizing DNA with enhanced accessibility. TC1 enables direct immobilization of DNA onto a solid support through self‐catalytic DNA covalent coupling and robust solid adsorption capabilities. This method demonstrates high accessibility to target molecules, supported by the improved sensitivity of DNA hybridization and aptamer‐target recognition assays. TC1‐mediated DNA immobilization is a one‐pot reaction that does not require chemical derivatives, making it promising for the development of high‐performance DNA materials and technologies.