Bifunctional utilization of whey powder as a substrate and inducer for β-farnesene production in an engineered Escherichia coli

Ding, Juanjuan; You, Shengping; Ba, Wenyan; Zhang, Hongtao; Chang, Hongxing; Qi, Wei; Su, Rongxin; He, Zhimin

doi:10.1016/j.biortech.2021.125739

Cited by 12 publications

(6 citation statements)

References 24 publications

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“…Whey, a by‐product of the dairy industry, is an environmental pollutant. [ 12 ] However, whey has lactose and abundant nitrogen sources. Thus, whey is a suitable material for microbial fermentation.…”

Section: Discussionmentioning

confidence: 99%

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Production of _D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess

Ma,

Li,

et al. 2024

Biotechnology Journal

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We designed and constructed a green and sustainable bioprocess to efficiently coproduce D‐tagatose, bioethanol, and microbial protein from whey powder. First, a one‐pot biosynthesis process involving lactose hydrolysis and D‐galactose redox reactions for D‐tagatose production was established in vitro via a three‐enzyme cascade. Second, a nicotinamide adenine dinucleotide phosphate‐dependent galactitol dehydrogenase mutant, D36A/I37R, based on the nicotinamide adenine dinucleotide‐dependent polyol dehydrogenase from Paracoccus denitrificans was created through rational design and screening. Moreover, an NADPH recycling module was created in the oxidoreductive pathway, and the tagatose yield increased by 3.35‐fold compared with that achieved through the pathway without the cofactor cycle. The reaction process was accelerated using an enzyme assembly with a glycine–serine linker, and the tagatose production rate was 9.28‐fold higher than the initial yield. Finally, Saccharomyces cerevisiae was introduced into the reaction solution, and 266.5 g of D‐tagatose, 162.6 g of bioethanol, and 215.4 g of dry yeast (including 38% protein) were obtained from 1 kg of whey powder (including 810 g lactose). This study provides a promising sustainable process for functional food (D‐tagatose) production. Moreover, this process fully utilized whey powder, demonstrating good atom economy.

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Section: Discussionmentioning

confidence: 99%

“…Global production is estimated to be approximately 160 million tons per year. [ 12 ] Whey is an ideal substrate for D ‐tagatose production because it is lactose‐rich. Simultaneously, whey contains proteins, fats, trace elements, and other nutrients that facilitate microbial fermentation.…”

Section: Introductionmentioning

confidence: 99%

Production of _D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess

Ma,

Li,

et al. 2024

Biotechnology Journal

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“…However, they observed a significant decrease in cell capability and pH as the storage period increased from 1 to 2 weeks under refrigeration conditions. Furthermore, Ding et al (2021) generated a novel technique to produce b-farnesene using a modified E. coli strain F13 that utilises whey powder bifunctionally. They determined that by utilising whey powder, they could produce 4.74 g L À1 of b-farnesene, 197% of the amount produced in shake flasks.…”

Section: Potential Applications Of Cw For Various Product Formulationsmentioning

confidence: 99%

“…Furthermore, Ding et al . (2021) generated a novel technique to produce β‐farnesene using a modified E. coli strain F13 that utilises whey powder bifunctionally. They determined that by utilising whey powder, they could produce 4.74 g L −1 of β‐farnesene, 197% of the amount produced in shake flasks.…”

Section: Potential Applications Of Cw For Various Product Formulationsmentioning

confidence: 99%

A recent glance on the valorisation of cheese whey for industrial prerogative: high‐value‐added products development and integrated reutilising strategies

Arshad

Hassan

Ahmad³

et al. 2022

Int J of Food Sci Tech

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Summary This review article explores recent advancements in converting cheese whey (CW) into high‐value‐added products, focusing on producing biofuels, bioelectricity and different whey‐based food items, as well as other potential uses. Additionally, new integrated reutilising strategies are proposed to utilise CW to develop innovative products with various applications, mainly focusing on sustainable alternatives at the industrial level. CW could be utilised with the help of different processes, for example separation of main protein components from cheese whey, like separation and purification of α‐lactalbumin, production of polysaccharides (lactose and lactulose), volatile fatty acids (VFAs), other organic acids and bioethanol. Besides these products, CW could also be utilised in the fermentation process, in the production of alcoholic drinks (whey sprite and white whisky), in the production of whey protein concentrate and as poultry feed. Biotechnological approaches for whey utilisation are the most recent alternatives. They can produce important products for the industries, such as whey‐based products, bioplastics, biofuels, bioelectricity, organic acids, bioactive compounds and whey‐based food items.

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“…Therefore, new alternatives for dealing with this challenge are still welcome, especially those focused on whey utilization as substrate for metabolites production. For example, the production of recombinant proteins [ 28 ], d -tagatose [ 29 ], hydrogen [ 30 ], ethanol [ 31 ], galacto-oligosaccharides [ 32 ], THR and l -lysine [ 33 ], using whey as substrate has been reported mainly by the action of E. coli [ 28 , 29 , 34 , 35 ], Saccharomyces fragilis [ 36 ], Aspergillus oryzae and Kluyveromyces lactis [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

l-Threonine production from whey and fish hydrolysate by E. coli ATCC® 21277

Vélez Blandón,

Sánchez Henao,

Zapata Montoya

et al. 2023

Heliyon

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Bifunctional utilization of whey powder as a substrate and inducer for β-farnesene production in an engineered Escherichia coli

Cited by 12 publications

References 24 publications

Production of _D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess

Production of _D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess

A recent glance on the valorisation of cheese whey for industrial prerogative: high‐value‐added products development and integrated reutilising strategies

l-Threonine production from whey and fish hydrolysate by E. coli ATCC® 21277

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Bifunctional utilization of whey powder as a substrate and inducer for β-farnesene production in an engineered Escherichia coli

Cited by 12 publications

References 24 publications

Production of D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess

Production of D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess

A recent glance on the valorisation of cheese whey for industrial prerogative: high‐value‐added products development and integrated reutilising strategies

l-Threonine production from whey and fish hydrolysate by E. coli ATCC® 21277

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Product

Resources

About

Production of _D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess

Production of _D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess