BIFURCATE FLOWER TRUSS: a novel locus controlling inflorescence branching in tomato contains a defective MAP kinase gene

Ferreira, Demetryus Silva; Kevei, Zoltán; Kurowski, Tomasz J.; Fonseca, Maria Esther de Noronha; Mohareb, Fady; Boiteux, L. S.; Thompson, Andrew J.

doi:10.1093/jxb/ery076

Cited by 6 publications

(4 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genomic DNA was extracted from young cotyledons/leaves, and the KASP/KBD assays were performed as described (Silva Ferreira et al 2018 ). KBD assays were developed by LGC (Teddington, UK) based on the provided SNP and flanking sequence data (Table S1 ).…”

Section: Methodsmentioning

confidence: 99%

Genes involved in auxin biosynthesis, transport and signalling underlie the extreme adventitious root phenotype of the tomato aer mutant

Kevei,

Larriba,

Romero-Bosquet

et al. 2024

Theor Appl Genet

Self Cite

View full text Add to dashboard Cite

The use of tomato rootstocks has helped to alleviate the soaring abiotic stresses provoked by the adverse effects of climate change. Lateral and adventitious roots can improve topsoil exploration and nutrient uptake, shoot biomass and resulting overall yield. It is essential to understand the genetic basis of root structure development and how lateral and adventitious roots are produced. Existing mutant lines with specific root phenotypes are an excellent resource to analyse and comprehend the molecular basis of root developmental traits. The tomato aerial roots (aer) mutant exhibits an extreme adventitious rooting phenotype on the primary stem. It is known that this phenotype is associated with restricted polar auxin transport from the juvenile to the more mature stem, but prior to this study, the genetic loci responsible for the aer phenotype were unknown. We used genomic approaches to define the polygenic nature of the aer phenotype and provide evidence that increased expression of specific auxin biosynthesis, transport and signalling genes in different loci causes the initiation of adventitious root primordia in tomato stems. Our results allow the selection of different levels of adventitious rooting using molecular markers, potentially contributing to rootstock breeding strategies in grafted vegetable crops, especially in tomato. In crops vegetatively propagated as cuttings, such as fruit trees and cane fruits, orthologous genes may be useful for the selection of cultivars more amenable to propagation.

show abstract

Section: Methodsmentioning

confidence: 99%

Genes involved in auxin biosynthesis, transport and signalling underlie the extreme adventitious root phenotype of the tomato aer mutant

Kevei,

Larriba,

Romero-Bosquet

et al. 2024

Theor Appl Genet

Self Cite

View full text Add to dashboard Cite

show abstract

“…Before germination, all seeds were sterilised in 0.45% w/v sodium hypochlorite for 30 min and then rinsed in tap water to avoid seed-borne viral transmission. Seed were germinated as described (Silva Ferreira et al, 2018 ) before transplanting into 8 L pots of Sinclair multipurpose compost (LBS Horticulture, Colne, UK). Pots were irrigated according to demand and were fed twice a week with Hoagland solution (5 mM K 2 SO 4 ; 1 mM H 3 PO 4 ; 5 mM Ca (NO 3 ) 2 ; 2 mM MgSO 4; 100 μM EDTA Fe-Na; 42.2 μM H 3 BO 3 ; 9.1 μM MnCl 2 ; 0.76 μM ZnSO 4 and 0.32 μM CuSO 4 , pH 5.8 adjusted with H 3 PO 4 ), at half strength before flowering and full strength after flowering.…”

Section: Methodsmentioning

confidence: 99%

“…Genomic DNA extraction from young leaves and the KASP/KBD assays were performed as described (Silva Ferreira et al, 2018 ). All KBD assays were developed by LGC (Teddington, UK) based on the provided nucleotide polymorphism and flanking sequence data (Table S1 ).…”

Section: Methodsmentioning

confidence: 99%

Missense mutation of a class B heat shock factor is responsible for the tomato bushy root-2 phenotype

et al. 2022

Self Cite

View full text Add to dashboard Cite

The bushy root-2 (brt-2) tomato mutant has twisting roots, and slower plant development. Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine (S75C) substitution in the DNA binding domain (DBD) of a heat shock factor class B (HsfB) encoded by SolycHsfB4a. This gene is orthologous to the Arabidopsis SCHIZORIZA gene, also known as AtHsfB4. The brt-2 phenotype is very similar to Arabidopsis lines in which the function of AtHsfB4 is altered: a proliferation of lateral root cap and root meristematic tissues, and a tendency for lateral root cap cells to easily separate. The brt-2 S75C mutation is unusual because all other reported amino acid substitutions in the highly conserved DBD of eukaryotic heat shock factors are dominant negative mutations, but brt-2 is recessive. We further show through reciprocal grafting that brt-2 exerts its effects predominantly through the root genotype even through BRT-2 is expressed at similar levels in both root and shoot meristems. Since AtHsfB4 is induced by root knot nematodes (RKN), and loss-of-function mutants of this gene are resistant to RKNs, BRT-2 could be a target gene for RKN resistance, an important trait in tomato rootstock breeding.Gene & accession numbersSolycHsfB4a - Solyc04g078770.

show abstract

“…In addition, the transcription factor SlZFP2 and protein kinase SlMAPK1 could also regulate branching in tomato. Overexpression of SlZFP2 gene increased the length of lateral branches and the SlMAPK1 mutation increased the number of lateral branches (Weng et al, 2016;Silva et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The Branchless Gene C lbl in Watermelon Encoding a Terminal Flower 1 Protein Regulate S the Number of Lateral Branch

Dou

Yang

Sun

et al. 2021

Preprint

View full text Add to dashboard Cite

Lateral branching is one of the most important traits, which directly determines plant 27 architecture and crop productivity. Commercial watermelon has the characteristics of multiple 28 lateral branches, and it is time-consuming and labor costing to manually remove the lateral 29 branches in traditional watermelon cultivation. In our present study, a lateral branchless trait was 30 identified in watermelon material W CZ, and genetic analysis revealed that it was controlled by a 31 single recessive gene, which named as Clbl . A bulked segregant sequencing (BSA seq) and 32 linkage analysis was conducted to primarily mapping of Clbl on watermelon chromosome 4 33 Next-generation sequencing aided marker discovery and a large mapping population consisting of 34 1406 F 2 plants was used to further mapped the Clbl locus into a 9011 bp candidate region which 35 harbored only one candidate gene Cla018392 encoding a TERMINAL FLOWER 1 gene. Sequence 36 comparison of Cla018392 between two parental lines revealed that there was a SNP detected from 37 C to A in the coding region in the branchless inbred line WCZ , which resulted in a mutation of 38 Alanine (GCA) to Glutamate (GAA) at the fourth exon A dCAPS marker was developed from the 39 SNP locus, which was co-segregated with the branchless phenotype in both BC 1 and F 2 population, 40 and it was also further validated in 152 natural watermelon accessions. qRT PCR and in situ 41 hybridization showed that the expression levels of Cla0 18392 was significantly reduced in the 42 axillary bud and apical bud in the branchless line WCZ Ectopic expression of ClTFL1 in 43 Arabidopsis showed an increased number of lateral branches. The results of this study will be 44 useful for better understanding the molecular mechanism of lateral branch development in 45 watermelon and for the development of marker-assisted selection (MAS) for new branchless 46 watermelon cultivars.

show abstract

BIFURCATE FLOWER TRUSS: a novel locus controlling inflorescence branching in tomato contains a defective MAP kinase gene

Abstract: The bifurcate flower truss (bif) gene, originating from Solanum galapagense, increases tomato inflorescence branching and flower number. A gene encoding a truncated MAP kinase is a strong candidate for bif.

Cited by 6 publications

References 82 publications

Genes involved in auxin biosynthesis, transport and signalling underlie the extreme adventitious root phenotype of the tomato aer mutant

Genes involved in auxin biosynthesis, transport and signalling underlie the extreme adventitious root phenotype of the tomato aer mutant

Missense mutation of a class B heat shock factor is responsible for the tomato bushy root-2 phenotype

The Branchless Gene C lbl in Watermelon Encoding a Terminal Flower 1 Protein Regulate S the Number of Lateral Branch

Contact Info

Product

Resources

About