Herein, the effect of carbon fibers (CFs) on the tensile property of a polylactic acid (PLA) specimen prepared by utilizing the fused deposition modeling (FDM) method, is investigated. The tensile property, crystal structure, and morphology of FDM-produced specimens were detected by universal testing machine, X-ray diffraction (XRD), and scanning electron microscopy (SEM), respectively. Meanwhile, the reinforcement mechanism of CFs on the FDM-printed PLA specimens was also studied. The DSC curves indicated that the crystalline structure of the PLA-CF specimen was higher than the PLA specimen. After the introduction of CFs, the XRD results showed the crystal structure of PLA varied from non-crystalline to α crystalline, and the SEM results illustrated the terrible bonding interface between carbon fiber and PLA. Interestingly, after the introduction of carbon fiber, the tensile strength of the PLA specimen reduced from 54.51 to 49.41 MPa. However, compared with the PLA component, the Young’s modulus and the elongation-at-break of the PLA-CF specimen increased from 1.04 GPa and 6.26%, to 1.26 GPa and 7.81%, respectively.