BIGEL Analysis of Gene Expression in HL60 Cells Exposed to X Rays or 60 Hz Magnetic Fields

Balcer‐Kubiczek, Elizabeth K.; Zhang, Xiao Feng; Han, Lin; Harrison, Greg J.; Davis, Christophér C.; Zhou, Xiao Juan; Ioffe, Vladimir; McCready, Welton A.; Abraham, John M.; Meltzer, Stephen J.

doi:10.2307/3579889

Cited by 9 publications

(3 citation statements)

References 52 publications

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“…2). Many genes already published as induced by ionizing radiation, including ACTB [Woloschak et al, 1990] encoding betaactin, CAT [Hardmeier et al, 1997] encoding catalase, COX6B and COX7B [Balcer-Kubiczek et al, 1998] encoding cytochrome-c oxidase, or HSP27 [Fornace et al, 1989] encoding a heat shock protein, were found to be upregulated in our study (see Table I and supplemental data), thus validating our experimental design.…”

Section: Global Expression Changes In G-irradiatedsupporting

confidence: 71%

Activation of an energy providing response in human keratinocytes after γ irradiation

Lamartine¹,

Franco²,

Minter³

et al. 2005

J. Cell. Biochem.

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We performed a microarray study on human differentiated HaCaT keratinocytes exposed to ionizing radiation (2 or 10 Gy). At 3 h after exposure, more than 150 known and unknown genes were found regulated in irradiated HaCaT keratinocytes. Among the genes regulated at 3 h, those involved in cell energy metabolism appeared to be the most abundant and the most responsive. Two mitochondrial ATP-synthases and several other genes involved in energy producing pathways, such as glucose metabolism, were induced, whereas many genes from energy requiring pathways were shut down. These changes in energy metabolism were confirmed both in normal primary keratinocytes and in HaCaT keratinocytes by RT-PCR and proteins studies. Moreover, measures of intracellular ATP revealed a 50% increase in keratinocytes immediately after irradiation, supporting an energy procurement response. The overall results indicate that irradiation induces an immediate burst of ATP that seems to be a general response of human differentiated keratinocytes to the radiation stress. This article contains Supplementary Material available at http://www.mrw.interscience.wiley.com/suppmat/0730-2312/suppmat/v95.html

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Section: Global Expression Changes In G-irradiatedsupporting

confidence: 71%

Activation of an energy providing response in human keratinocytes after γ irradiation

Lamartine¹,

Franco²,

Minter³

et al. 2005

J. Cell. Biochem.

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“…Its up‐regulation could be involved in the affected neurons' apparent resistance to undergo the complete apoptotic program. Neuroleukin is up‐regulated in HL60 cells exposed to x‐irradiation (Balcer‐Kubiczek et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Early Alterations in Gene Expression and Cell Morphology in a Mouse Model of Huntington's Disease

Iannicola¹,

Moreno²,

Oliverio³

et al. 2000

Journal of Neurochemistry

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Several mouse models for Huntington's disease (HD) have been produced to date. Based on differences in strain, promoter, construct, and number of glutamines, these models have provided a broad spectrum of neurological symptoms, ranging from simple increases in aggressiveness with no signs of neuropathology, to tremors and seizures in absence of degeneration, to neurological symptoms in the presence of gliosis and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) positivity, and finally to selective striatal damage associated with electrophysiological and behavioral abnormalities. We decided to analyze the morphology of striatum and hippocampus from a mouse transgenic line obtained by microinjection of exon 1 from the HD gene after introduction of a very high number of CAG repeat units. We found a massive darkening and compacting of striatal and hippocampal neurons in affected mice, associated with a lower degree of more classical apoptotic cell condensation. We then explored whether this morphology could be explained with alterations in gene expression by hybridizing normal and affected total brain RNA to a panel of 588 known mouse cDNAs. We show that some genes are significantly and consistently up-regulated and that others are down-regulated in the affected brains. Here we discuss the possible significance of these alterations in neuronal morphology and gene expression.

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“…BNIP3 as a member of the BCL2 family has been implicated in counteracting the upstream initiation of the caspase activation cascade by interfering with cytochrome c release from mitochondrion [58,65]; we previously observed a coassociation between BCL2 and BNIP3 in human leukemia cells [57]. As reported [57], mRNA levels of both BCL2 and BNIP3 have been strongly reduced in response to apoptotic stimuli.…”

Section: Discussionmentioning

confidence: 50%

Survival of colorectal cancer cell lines treated with paclitaxel, radiation, and 5-FU: Effect of TP53 or hMLH1 deficiency

et al. 2000

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BIGEL Analysis of Gene Expression in HL60 Cells Exposed to X Rays or 60 Hz Magnetic Fields

Cited by 9 publications

References 52 publications

Activation of an energy providing response in human keratinocytes after γ irradiation

Activation of an energy providing response in human keratinocytes after γ irradiation

Early Alterations in Gene Expression and Cell Morphology in a Mouse Model of Huntington's Disease

Survival of colorectal cancer cell lines treated with paclitaxel, radiation, and 5-FU: Effect of TP53 or hMLH1 deficiency

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