Bigger is not always better: Transmission and fitness burden of ∼1MB Pseudomonas syringae megaplasmid pMPPla107

Romanchuk, Artur; Jones, Corbin D.; Karkare, Kedar; Moore, Autumn; Smith, Brian A.; Jones, Chelsea K.; Dougherty, Kevin; Baltrus, David A.

doi:10.1016/j.plasmid.2014.04.002

Cited by 41 publications

(68 citation statements)

References 48 publications

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“…In a parallel manuscript [18], we demonstrate that acquisition of this megaplasmid lowers fitness of Pseudomonas stutzeri by ∼20% and here we report on multiple additional phenotypes affected by large-scale HGT. Specifically, we find that megaplasmid acquisition leads to sensitivity to quinolone antibiotics, DNA intercalating agents, temperature, and killing by other bacterial species.…”

Section: Introductionsupporting

confidence: 64%

“…In a separate manuscript [18], we have demonstrated that megaplasmid acquisition by P. stutzeri impacts competitive fitness and bacterial growth under standard laboratory conditions. Here we show that megaplasmid acquisition is also accompanied by secondary changes to a variety of phenotypes affecting cellular physiology, environmental survival, and interactions with other species.…”

Section: Discussionmentioning

confidence: 89%

“…Furthermore, even when costs are observed, measurements are often limited to single phenotypes even though multiple cellular systems could be affected [5], [6], [13], [28]–[30]. Here we explore a system where HGT increases bacterial genome size by ∼20%, using a megaplasmid which is self-transmissible throughout pseudomonads [18], [19]. We report that megaplasmid acquisition alters numerous phenotypes within P. stutzeri in unprecedented ways, which highlights the potential for large-scale transfers to shape evolutionary dynamics within natural populations.…”

Section: Discussionmentioning

confidence: 99%

“…We have developed an experimental system to investigate the costs of large-scale HGT by using a ∼1Mb megaplasmid which is self-transmissible throughout Pseudomonas species [18]. Transfer of this megaplasmid occurs in both liquid and solid media and requires a type IV secretion system.…”

Section: Introductionmentioning

confidence: 99%

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Multiple Phenotypic Changes Associated with Large-Scale Horizontal Gene Transfer

et al. 2014

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Horizontal gene transfer often leads to phenotypic changes within recipient organisms independent of any immediate evolutionary benefits. While secondary phenotypic effects of horizontal transfer (i.e., changes in growth rates) have been demonstrated and studied across a variety of systems using relatively small plasmids and phage, little is known about the magnitude or number of such costs after the transfer of larger regions. Here we describe numerous phenotypic changes that occur after a large-scale horizontal transfer event (∼1 Mb megaplasmid) within Pseudomonas stutzeri including sensitization to various stresses as well as changes in bacterial behavior. These results highlight the power of horizontal transfer to shift pleiotropic relationships and cellular networks within bacterial genomes. They also provide an important context for how secondary effects of transfer can bias evolutionary trajectories and interactions between species. Lastly, these results and system provide a foundation to investigate evolutionary consequences in real time as newly acquired regions are ameliorated and integrated into new genomic contexts.

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Section: Introductionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiple Phenotypic Changes Associated with Large-Scale Horizontal Gene Transfer

et al. 2014

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“…It was later shown that pMPPla107 is selftransmissible across a variety of diverse pseudomonad strains with conjugation dependent on a Type Four Secretion System (T4SS). However, its role in virulence remains elusive (Romanchuk et al 2014). Recently, Baltrus et al (2017) described four different Type Three Secretion Systems (T3SS) in P. syringae pathovars: canonical, rhizobial, single and atypical.…”

Section: Introductionmentioning

confidence: 99%

Genome analysis of Pseudomonas syringae pv. lachrymans strain 814/98 indicates diversity within the pathovar

et al. 2017

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Although many Pseudomonas syringae strains have already been determined, only a few genomes of strains belonging to pathovar lachrymans have been sequenced so far. In this study we report the genome sequence of P. syringae pv. lachrymans strain 814/98, which is highly virulent to cucumber. The genome size was estimated to be 6.58 Mb, with 57.97% GC content. In total, 6024 genes encoding proteins and 92 genes encoding RNAs were identified in this genome. Comparisons with the available sequenced genomes of pathovar lachrymans as well as with other P. syringae pathovars were conducted, revealing the presence of three unique plasmids and 24 type III effector proteins (TTEs) in strain 814/98. The phylogenetic analyses of MLST loci and TTEs clearly showed the existence of two distinct clusters of strains within pathovar lachrymans, which were grouped into either phylogroup 1 or 3, supporting non-monophyly within this pathovar.

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The metabolic burden associated with plasmid acquisition: An assessment of the unrecognized benefits to host cells

Curtsinger,

Martínez‐Absalón,

Liu

et al. 2024

BioEssays

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Bacterial conjugation, wherein DNA is transferred between cells through direct contact, is highly prevalent in complex microbial communities and is responsible for spreading myriad genes related to human and environmental health. Despite their importance, much remains unknown regarding the mechanisms driving the spread and persistence of these plasmids in situ. Studies have demonstrated that transferring, acquiring, and maintaining a plasmid imposes a significant metabolic burden on the host. Simultaneously, emerging evidence suggests that the presence of a conjugative plasmid can also provide both obvious and unexpected benefits to their host and local community. Combined, this highlights a continuous cost‐benefit tradeoff at the population level, likely contributing to overall plasmid abundance and long‐term persistence. Yet, while the metabolic burdens of plasmid conjugation, and their causes, are widely studied, their attendant potential advantages are less clear. Here, we summarize current perspectives on conjugative plasmids’ metabolic burden and then highlight the lesser‐appreciated yet critical benefits that plasmid‐mediated metabolic burdens may provide. We argue that this largely unexplored tradeoff is critical to both a fundamental theory of microbial populations and engineering applications and therefore warrants further detailed study.

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Bigger is not always better: Transmission and fitness burden of ∼1MB Pseudomonas syringae megaplasmid pMPPla107

Cited by 41 publications

References 48 publications

Multiple Phenotypic Changes Associated with Large-Scale Horizontal Gene Transfer

Multiple Phenotypic Changes Associated with Large-Scale Horizontal Gene Transfer

Genome analysis of Pseudomonas syringae pv. lachrymans strain 814/98 indicates diversity within the pathovar

The metabolic burden associated with plasmid acquisition: An assessment of the unrecognized benefits to host cells

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