Bilateração e geometria de distâncias

Bridging the gap: functional healing of embryonic small intestineex vivo

“…The current results confirm reports (Abud et al, 2005;Quinlan et al, 2006) that embryonic murine intestine can be maintained in organ culture, an environment permitting growth and differentiation. Abud et al (2005) used this system to implement signalling through the epidermal growth factor in stimulation of epithelial growth and survival. Quinlan et al (2006) showed that reporter genes could be virally transduced into cultured embryonic intestinal explants.…”

“…In addition, ex vivo organ culture can be used to assess the effects on organogenesis of physical manipulations or the addition of exogenous chemicals, such as growth factors. With respect to the mammalian small intestine, previous studies have begun to explore these aspects using, as examples, organ culture of intact embryonic gut rudiments (Abud et al, 2005;Quinlan et al, 2006) and the generation of gut organoids derived from intestinal stem cells (Sato and Clevers, 2013). Our study aimed to create an organ culture model with which to test physical manipulations to enhance healing of small intestinal rudiments, thus generating a single functional organ.…”