Bilateral asymmetry in bullfrog testes and fat bodies: correlations with steroidogenic activity, mast cells number and structural proteins

Dias, Andressa Baggio; Oliveira, Salmo Azambuja de; Cerri, Paulo Sérgio; Sasso‐Cerri, Estela

doi:10.1016/j.acthis.2022.151873

Cited by 6 publications

(2 citation statements)

References 54 publications

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“…In our previous work, it has been proved that E 2 induces an increase of interstitial connective tissue, together with MCN in the HG of the frog, Pelophylax esculentus (Di Matteo et al, 1995). On the contrary, several experimental data proved that testosterone negatively correlated with MCN (Dias et al, 2022). For instance, the use of ethane dimethane sulphonate, an alkylating agent that selectively disrupts Leydig cells (Venditti et al, 2021c), increased MCN in testis (Di Matteo et al, 1992; Minucci et al, 1992) and in the HG (Di Matteo et al, 2000).…”

Section: Resultsmentioning

confidence: 97%

Preliminary study of the ameliorative effects of melatonin on cadmium‐induced morphological and biochemical alterations in the rat Harderian gland

et al. 2022

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Herein is reported, for the first time in the rat Harderian gland (HG), the counteractive action of melatonin (Mlt), a well-known antioxidant radical scavenger, on the increased oxidative stress damages induced by a pro-oxidant substance, cadmium (Cd), an environmental pollutant also considered as endocrine disruptor.HG, an infraorbital gland present in almost all terrestrial vertebrates, produces a lipid secretion to lubricate the eyeball, as well as porphyrin/Mlt as light transducers.Moreover, HG is an extra-gonadal source of steroid sex hormones. Via ex vivo experiments lasting for 24 h, we verified the increased lipid peroxidation in Cd-treated glands, producing morphological alteration of the glandular epithelium, as well as an increased porphyrins accumulation. Moreover, Cd also induced a decreased protein level of the steroidogenic enzymes steroidogenic acute regulatory (StAR) and 3βHSD, and an increased mast cell number. Results obtained with Mlt cotreatment demonstrated that it decreased the levels of Cd-induced oxidative damage, with reversal of all the observed modification. Furthermore, the TUNEL assay showed that the increased number of apoptotic cells in Cd-treated HG was counteracted by the contemporaneous Mlt administration. Results confirmed that Mlt treatment restored the levels of two autophagy markers, LC3 and p62, counteracting the autophagy Cd-induced. Interestingly, the positive effects of Mlt alone were highlighted by the decreased rate of lipid peroxidation as compared with the control, confirming its antioxidant action. Combined data further confirmed the antioxidant action of Mlt in counteracting the degeneration provoked by reactive oxygen species (ROS) in the rat HG, a tissue extremely susceptible to oxidative stress condition.

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Section: Resultsmentioning

confidence: 97%

Preliminary study of the ameliorative effects of melatonin on cadmium‐induced morphological and biochemical alterations in the rat Harderian gland

et al. 2022

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“…Our previous papers reported that E2 induces an increase in interstitial connective tissue along with MCN in the HG of the frog Pelophylax esculentus (Di Matteo et al, 1995). In contrast, several experiments demonstrated that testosterone correlated negatively with MCN (Dias et al, 2022). We recently observed an increase in MCN in the HG of Cd-treated rats, and this might be due to the characteristic of the metal being considered an endocrine disruptor, which by mimicking estrogen activates its receptors (Romano et al, 2022).…”

Section: Resultsmentioning

confidence: 97%

Morphological and molecular changes in the Harderian gland of streptozotocin‐induced diabetic rats

et al. 2023

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Using a rat model of type 1 diabetes (T1D) obtained by treatment with streptozotocin, an antibiotic that destroys pancreatic β‐cells, we evaluated the influence of subsequent hyperglycemia on the morphology and physiology of the Harderian gland (HG). HG is located in the medial corner of the orbit of many terrestrial vertebrates and, in rodents, is characterized by the presence of porphyrins, which being involved in the phototransduction, through photo‐oxidation, produce reactive oxygen species activating the autophagy pathway. The study focused on the expression of some morphological markers involved in cell junction formation (occludin, connexin‐43, and α‐tubulin) and mast cell number (MCN), as well as autophagic and apoptotic pathways. The expression of enzymes involved in steroidogenesis [steroidogenic acute regulatory protein (StAR), and 3β‐hydroxysteroid dehydrogenase (3β‐HSD)] and the level of lipid peroxidation by thiobarbituric acid reactive species assay were also evaluated. The results strongly indicate, for the first time, that T1D has a negative impact on the pathophysiology of rat HG, as evidenced by increased oxidative stress, morphological and biochemical alterations, hyperproduction and secretion of porphyrins, increased MCN, reduced protein levels of StAR and 3β‐HSD, and, finally, induced autophagy and apoptosis. All the combined data support the use of the rat HG as a suitable experimental model to elucidate the molecular damage/survival pathways elicited by stress conditions.

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Hormones and testicular function in amphibians

Marlatt

2024

Hormones and Reproduction of Vertebrates, Volume 2

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Bilateral asymmetry in bullfrog testes and fat bodies: correlations with steroidogenic activity, mast cells number and structural proteins

Cited by 6 publications

References 54 publications

Preliminary study of the ameliorative effects of melatonin on cadmium‐induced morphological and biochemical alterations in the rat Harderian gland

Preliminary study of the ameliorative effects of melatonin on cadmium‐induced morphological and biochemical alterations in the rat Harderian gland

Morphological and molecular changes in the Harderian gland of streptozotocin‐induced diabetic rats

Hormones and testicular function in amphibians

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