In this paper, we establish some new results on the existence of positive solutions for a singular tempered sub-diffusion fractional equation involving a changing-sign perturbation and a lower-order sub-diffusion term of the unknown function. By employing multiple transformations, we transform the changing-sign singular perturbation problem to a positive problem, then establish some sufficient conditions for the existence of positive solutions of the problem. The asymptotic properties of solutions are also derived. In deriving the results, we only require that the singular perturbation term satisfies the Carathéodory condition, which means that the disturbance influence is significant and may even achieve negative infinity near some time singular points.