Bilayer Lipid Membrane as Memcapacitance: Capacitance–Voltage Pinched Hysteresis and Negative Insertion Conductance

Smirnova, E. Yu.; Аносов, А. А.

doi:10.3390/membranes13010097

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…The measurement method of the surface potential exploits the nonmonotonic voltage dependence of membrane capacitance [ 28 , 29 , 30 ]. In [ 29 ], it was shown that the voltage corresponding to the minimum of the capacitance current could be uniquely calculated using current responses to upward and downward half-periods of the triangular voltage. With an asymmetric addition of cyt c, the resulting difference in the surface potentials can be compensated for by an external command voltage.…”

Section: Methodsmentioning

confidence: 99%

“…A triangular alternating command voltage with amplitudes in the range of 100–200 mV and frequencies from 0.5 to 1 Hz was applied to the membrane and cyclic current-voltage characteristics were registered ( Figure 1 a). The half-difference of the current responses to upward and downward half-periods of the triangular voltage ( Figure 1 b) is proportional (if transitional processes are excluded) to the membrane capacitance [ 29 ]. To assess the difference in surface potentials for each concentration of cyt c and each pH, the voltage corresponding to the minimum half-difference of the current responses was recorded and compared with the corresponding data of the control membranes.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Cytochrome C on the Conductance of Asolectin Membranes and the Occurrence of Through Pores at Different pHs

et al. 2023

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The study of the electrical parameters of asolectin bilayer lipid membranes in the presence of cytochrome c (cyt c) at various concentrations showed that an increase in the concentration of cyt c leads to an increase in the membrane conductance and the appearance of through pores. The studied membranes did not contain cardiolipin, which is commonly used in studying the effect of cyt c on membrane permeability. In the presence of cyt c, discrete current fluctuations were recorded. The occurrence of these fluctuations may be associated with the formation of through pores. The diameter of these pores was ~0.8 nm, which is smaller than the size of the cyt c globule (~3 nm). Measurements carried out at pH values from 6.4 to 8.4 showed that the concentration dependence of the membrane conductance increases with increasing pH. To assess the binding of cyt c to the bilayer, we measured the concentration and pH dependences of the difference in surface potentials induced by the unilateral addition of cyt c. The amount of bound cyt c at the same concentrations decreased with increasing pH, which did not correspond to the conductance trend. An analysis of conductance traces leads to the conclusion that an increase in the integral conductance of membranes is associated with an increase in the lifetime of pores. The formation of “long-lived” pores, of which the residence time in the open state is longer than in the closed state, was achieved at various combinations of pHs and cyt c concentrations: the higher the pH, the lower the concentration at which the long-lived pores appeared and, accordingly, a higher conductance was observed. The increase in conductance and the formation of transmembrane pores are not due to the electrostatic interaction between cyt c and the membrane. We hypothesize that an increase in pH leads to a weakening of hydrogen bonds between lipid heads, which allows cyt c molecules to penetrate into the membrane. This disrupts the order of the bilayer and leads to the occurrence of through pores.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of Cytochrome C on the Conductance of Asolectin Membranes and the Occurrence of Through Pores at Different pHs

et al. 2023

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Effect of colloidal magnetite (Fe3O4) nanoparticles on the electrical characteristics of the azolectin bilayer in a static inhomogeneous magnetic field

Anosov,

Smirnova,

Sukhova

et al. 2024

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Penetration of hydrophilic colloidal magnetite nanoparticles through a lipid membrane in an inhomogeneous magnetic field

Anosov,

Borisova,

Smirnova

et al. 2024

Physics of Fluids

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In this study, we provide the first experimental evidence that colloidal hydrophilic magnetite nanoparticles can penetrate through bilayer lipid membrane in a non-uniform stationary magnetic field. Hydrophilic ligand-free cationic colloidal magnetite nanoparticles with an average diameter of 4 nm were added to the surrounding aqueous solution on one side of the azolectin membrane. An external non-uniform magnetic field ensured the attraction of superparamagnetic magnetite nanoparticles to the membrane, resulting in the formation of a near-membrane charged layer of cationic nanoparticles resulting in the initial polarization of the membrane. As a result of the passage of magnetite nanoparticles through the membrane, the polarization of the membrane decreases, and the membrane becomes depolarized. Independent methods were used to detect magnetite nanoparticles that passed through the lipid membrane including transmission electron microscopy and energy-dispersive x-ray spectroscopy. The discovered effect may be due to the following factors and interactions of nanoparticles. Interaction of magnetic nanoparticles with external inhomogeneous magnetic field provides localization of nanoparticles on the membrane surface. Collective interactions between nanoparticles, as well as their interactions with external electric and magnetic fields, lead to the formation of magnetite nanoparticle aggregates. Interaction of nanoparticles with the membrane lipid matrix leads to the formation of organic–inorganic complexes in which the polar surface of nanoparticles is enveloped by a lipid layer. The penetration of nanoparticles through the membrane is caused by the interaction of organic–inorganic complexes of nanoparticles and their aggregates with local intramembrane and near-membrane electric and magnetic fields.

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Bilayer Lipid Membrane as Memcapacitance: Capacitance–Voltage Pinched Hysteresis and Negative Insertion Conductance

Cited by 4 publications

References 35 publications

Effect of Cytochrome C on the Conductance of Asolectin Membranes and the Occurrence of Through Pores at Different pHs

Effect of Cytochrome C on the Conductance of Asolectin Membranes and the Occurrence of Through Pores at Different pHs

Effect of colloidal magnetite (Fe3O4) nanoparticles on the electrical characteristics of the azolectin bilayer in a static inhomogeneous magnetic field

Penetration of hydrophilic colloidal magnetite nanoparticles through a lipid membrane in an inhomogeneous magnetic field

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