Bilayered biodegradable poly(ethylene glycol)/poly(butylene terephthalate) copolymer (Polyactive?) as substrate for human fibroblasts and keratinocytes

Dorp, A.G.M. van; Verhoeven, Mary C.H.; Koerten, Henk K.; Blitterswijk, Clemens A. van; Ponec, Maria

doi:10.1002/(sici)1097-4636(19991205)47:3<292::aid-jbm2>3.0.co;2-b

Cited by 65 publications

(34 citation statements)

References 23 publications

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“…The inherent instability of these hydrogels due to the high mobility of the PEG chains may have inhibited the spreading of cell types exhibiting high tensile stress (such as skeletal muscle cells and fibroblasts 28 ) or may have changed the surface characteristics (e.g., surface charge), parameters that could be important for skeletal muscle cell attachment and/or growth. 8 On the other hand, the high water content of the PEGT/PBT compositions with a higher wt % of PEG induced differentiation of chondrocytes due to their rounded morphology on these substrates.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that PEGT/PBT formulations with a PEGT content of 70 wt % have bone-bonding properties 1 while polymers with a PEGT content of 55 wt % are optimal as skin substitutes. 8,9 Engineered (soft or hard) tissues could form the basis for novel therapies for patients suffering from the loss of tissue or its function. 10 One approach to the development of functional tissues is the introduction of isolated cells onto biodegradable polymer scaffolds that provide a defined structure for cell attachment and tissue organization and mimic the key properties of the tissue to be engineered.…”

Section: Introductionmentioning

confidence: 99%

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The different behaviors of skeletal muscle cells and chondrocytes on PEGT/PBT block copolymers are related to the surface properties of the substrate

Papadaki

Mahmood

Gupta

et al. 2000

J. Biomed. Mater. Res.

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The attachment, proliferation, morphology, and differentiation of two cell types-skeletal muscle cells and chondrocytes-were investigated on different compositions of poly(ethylene glycol) and poly(butylene terephthalate) segmented block copolymers. Four weight percentages (40, 55, 60, and 70%) and two different molecular weights (300 and 1000 Da) of poly(ethylene glycol) were tested. Varying the weight percentage and molecular weight of poly(ethylene glycol) resulted in different behaviors for skeletal muscle cells and chondrocytes. The attachment of skeletal muscle was the highest (similar to tissue culture polystyrene) when copolymers containing 55 wt % of poly(ethylene glycol) were used, regardless of the poly(ethylene glycol) molecular weight. Maximum proliferation and differentiation of skeletal muscle cells was achieved when copolymers containing 55 wt % and 300 Da molecular weight of poly(ethylene glycol) were used. In contrast, the weight percentage and molecular weight of poly(ethylene glycol) had no significant effect on chondrocyte attachment and proliferation; the attached chondrocytes retained a differentiated phenotype only when a 70 wt % of poly(ethylene glycol) was used. Cell behavior was correlated with the surface properties of the copolymer films, as indicated by contact-angle measurements. These results suggest that an optimized wt % and molecular weight of poly(ethylene glycol) will be useful depending on the specific cell type.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The different behaviors of skeletal muscle cells and chondrocytes on PEGT/PBT block copolymers are related to the surface properties of the substrate

Papadaki

Mahmood

Gupta

et al. 2000

J. Biomed. Mater. Res.

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“…This composition supported the growth of fibroblasts and keratinocytes. 20 The scaffolds were circular, with a diameter of 5 mm and a thickness of 220 -260 m and a porosity of 70 -80%. Pore size was varied with the following diameters: group 1, 20 -75 m; group 2, 75-212 m, group 3, 250 -300 m. Scaffold morphology from both sides of the scaffold is shown in Figure 2 using standard SEM analysis.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

Druecke

Langer

Lamme³

et al. 2003

J Biomedical Materials Res

173

138

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Poly(ether ester) block-copolymer scaffolds of different pore size were implanted into the dorsal skinfold chamber of balb/c mice. Using intravital fluorescent microscopy, the temporal course of neovascularization into these scaffolds was quantitatively analyzed. Three scaffold groups (diameter, 5 mm; 220 -260 thickness, m; n ϭ 30) were implanted. Different pore sizes were evaluated: small (20 -75 m), medium (75-212 m) and large pores (250 -300 m). Measurements were performed on days 8, 12, 16, and 20 in the surrounding normal tissue, in the border zone, and in the center of the scaffold. Standard microcirculatory parameters were assessed (plasma leakage, vessel diameter, red blood cell velocity, and functional vessel density). The largepored scaffolds showed significantly higher functional vessel density in the border zone and in the center (days 8 and 12) compared with the scaffold with the small and mediumsized pores. These data correlated with a larger vessel diameter and a higher red blood cell velocity in the largepored scaffold group. Interestingly, during the evaluation period the microcirculatory parameters on the edge of the scaffolds returned to values similar to those found in the surrounding tissue. In the center of the scaffold, however, neovascularization was still active 20 days after implantation. Plasma leakage and vessel diameter were higher in the center of the scaffold. Red blood cell velocity and functional vessel density were 50% lower than in the surrounding tissue. In conclusion, the dorsal skinfold chamber model in mice allows long-term study of blood vessel growth and remodeling in porous biomedical materials. The rate of vessel ingrowth into poly(ether ester) block-copolymer scaffolds is influenced by pore size and was highest in the scaffold with the largest pores. The data generated with this model contribute to knowledge about the development of functional vessels and tissue ingrowth into biomaterials.

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“…Nevertheless, the bilayered epidermal-dermal construct has been rarely developed on nanofibrous membranes. For its creation, other forms of synthetic and natural polymers have been used, for example a knitted PLGA mesh combined with collagen-hyaluronic acid sponge [96], porous scaffolds made of a copolymer of poly(ethylene glycol terephthalate) and poly(butylene terephthalate) [97] matrices containing fibrin, collagen, hyaluronan and their combinations [98][99][100], and even spider silk woven on steel frames [101]. Thus, we have attempted to develop a bilayer of keratinocytes and skin fibroblasts using nanofibrous PLA membranes, fixed in adapted CellCrown inserts (Scaffdex, Tampere, Finland).…”

Section: Nanofibers In Skin Tissue Engineeringmentioning

confidence: 99%

Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

Bačáková¹,

Bačáková²,

Pajorová³

et al. 2016

Nanofiber Research - Reaching New Heights

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Nanofibers are promising cell carriers for tissue engineering of a variety of tissues and organs in the human organism. They have been experimentally used for reconstruction of tissues of cardiovascular, respiratory, digestive, urinary, nervous and musculoskeletal systems. Nanofibers are also promising for drug and gene delivery, construction of biosensors and biostimulators, and wound dressings. Nanofibers can be created from a wide range of natural polymers or synthetic biostable and biodegradable polymers. For hard tissue engineering, polymeric nanofibers can be reinforced with various ceramic, metal-based or carbon-based nanoparticles, or created directly from hard materials. The nanofibrous scaffolds can be loaded with various bioactive molecules, such as growth, differentiation and angiogenic factors, or funcionalized with ligands for the cell adhesion receptors. This review also includes our experience in skin tissue engineering using nanofibers fabricated from polycaprolactone and its copolymer with polylactide, cellulose acetate, and particularly from polylactide nanofibers modified by plasma activation and fibrin coating. In addition, we studied the interaction of human bone-derived cells with nanofibrous scaffolds loaded with hydroxyapatite or diamond nanoparticles. We also created novel nanofibers based on diamond deposition on a SiO 2 template, and tested their effects on the adhesion, viability and growth of human vascular endothelial cells.

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Bilayered biodegradable poly(ethylene glycol)/poly(butylene terephthalate) copolymer (Polyactive?) as substrate for human fibroblasts and keratinocytes

Cited by 65 publications

References 23 publications

The different behaviors of skeletal muscle cells and chondrocytes on PEGT/PBT block copolymers are related to the surface properties of the substrate

The different behaviors of skeletal muscle cells and chondrocytes on PEGT/PBT block copolymers are related to the surface properties of the substrate

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

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