Bile acids in combination with low pH induce oxidative stress and oxidative DNA damage: relevance to the pathogenesis of Barrett's oesophagus

Dvorak, Katerina; Payne, Claire M.; Chavarria, Melissa; Ramsey, Lois; Dvorakova, Barbora; Bernstein, Harris; Holubec, Hana; Sampliner, Richard E.; Guy, Naihsuan; Condon, Amanda; Bernstein, Carol; Green, Sylvan B.; Prasad, Anil; Garewal, Harinder S.

doi:10.1136/gut.2006.103697

Cited by 224 publications

(214 citation statements)

References 51 publications

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“…The positive charge on the phosphonium group in MitoSOX Red selectively targets this cell-permeant HE derivative to mitochondria, where it accumulates as a function of mitochondrial membrane potential and exhibits fluorescence upon oxidation and subsequent binding to mitochondrial DNA 7 . In numerous recent studies, MitoSOX was validated with fluorescent/confocal microscopy 7,11-19 and flow cytometry 19-25 for selective detection of mitochondrial superoxide production in endothelial cells 18,19,25 , cardiomyocytes 16,19,22,25 , keratinocytes 11 , fibroblasts 17 , epithelial and lymphoid cells 20,21,23,24 , and neuronal cells 7,12-15 , to name just a few (see also Table 1 for more details), as well as in isolated vessels 26 .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy

et al. 2007

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Annexin V and Sytox Green are widely used markers to evaluate apoptosis in various cell types using flow cytometry and fluorescent microscopy. Recently, a novel fluoroprobe MitoSOX Red was introduced for selective detection of superoxide in the mitochondria of live cells and was validated for confocal microscopy and flow cytometry. This protocol describes simultaneous measurements of mitochondrial superoxide generation with apoptotic markers (Annexin V and Sytox Green) by both flow cytometry and confocal microscopy in endothelial cell lines. The advantages of the described flow cytometry method over other cell-based techniques are the tremendous speed (1-2 h), exquisite precision and the possibility of simultaneous quantitative measurements of mitochondrial superoxide generation and apoptotic (and other) markers, with maximal preservation of cellular functions. This method combined with fluorescent microscopy may be very useful to reveal important spatial-temporal changes in mitochondrial superoxide production and execution of programmed cell death in virtually any cell type.

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Section: Introductionmentioning

confidence: 99%

Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy

et al. 2007

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“…However, other factors such as nicotine from smoking, alcohol consumption, low pH, viruses, bacterial and carcinogens can also increase cancer risk. It is known that bile acids plus low pH, and bile acids plus nicotine act synergistically in increasing oxidative stress and DNA damage [68,69]. Since there is cross-talk among various stressresponse pathways, it is probable that other synergistic effects will be ascertained in the future.…”

Section: Synergistic Effects Of Bile Acids and Other Risk Factors In mentioning

confidence: 99%

Field defects in progression to gastrointestinal tract cancers

Bernstein

Payne

et al. 2008

Cancer Letters

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A field of defective tissue may represent a pre-malignant stage in progression to many cancers. However, field defects are often overlooked in studies of cancer progression through assuming tissue at some distance from the cancer is normal. We indicate, however, the generality of field defects in gastrointestinal cancers, including cancers of the oropharynx, esophagus, stomach, bile duct, pancreas, small intestine and colon/rectum. Common features of these field defects are reduced apoptosis competence, aberrant proliferation and genomic instability. These features are often associated with high bile acid exposure and may explain the association of dietary-related factors with cancer progression.

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“…La exposición del esófago distal al refl ujo ácido del estómago está usualmente asociado a EB, pero el refl ujo de biliar es más común en esta enfermedad 13 . Aunque la bilis por sí sola pareciera no contribuir, existiría un efecto sinérgico entre el ácido y la bilis en la aparición de metaplasia intestinal 14,15 . No existe evidencia que establezca una relación entre la infección por H. pylori y el EB, por lo que se requieren más estudios que aclaren esta asociación.…”

Section: Discussionunclassified

Efectos del bypass gástrico en el esófago de Barrett de pacientes con obesidad mórbida

et al. 2012

Rev Chil Cir

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Effects of gastric bypass for morbid obesity on Barrett esophagusBackground: Besides the weight reducing effects of gastric bypass, it is also a good antirefl ux procedure since there is no acid production by the gastric pouch and there is no duodenal refl ux due to the presence of a Roux en Y. Aim: To describe the effect of gastric bypass on Barrett esophagus among patients with morbid obesity. Material and Methods: Among 896 patients subjected to gastric bypass, 14 patient with a Barrett esophagus diagnosed with endoscopy and biopsy, were followed. A new endoscopy was performed one to 30 months after the surgical procedure. Results: Short (< = 30 mm) and long segment (> = 31 mm) Barrett esophagi were present in eight and six patients, respectively. Gastroesophageal refl ux symptoms relieved in 70% of these cases in a mean lapse of 6.5 months. There was regression from intestinal metaplasia to cardial mucosa in six patients (75%) with short-segment, and in one patient (16%) with long-segment Barrett esophagus. Conclusions: Gastric bypass in patients with morbid obesity and Barrett esophagus is a very good antirefl ux operation. This was proved by the disappearance of symptoms in almost all patients and by the regression of the intestinal metaplasia which is time and length dependent.

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Bile acids in combination with low pH induce oxidative stress and oxidative DNA damage: relevance to the pathogenesis of Barrett's oesophagus

Cited by 224 publications

References 51 publications

Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy

Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy

Field defects in progression to gastrointestinal tract cancers

Efectos del bypass gástrico en el esófago de Barrett de pacientes con obesidad mórbida

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