Bilirubin adsorption properties of water-soluble adsorbents with different cyclodextrin cavities in plasma dialysis system

Wang, Zhi; Cao, Yaming; Wei, Houliang; Jia, Lingyun; Xu, Li; Xie, Ju

doi:10.1016/j.colsurfb.2011.10.006

Cited by 31 publications

(17 citation statements)

References 27 publications

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“…11 β-Cyclodextrin was selected as the sorbent because it can sensitively recognize and efficiently bind a variety of hydrophobic molecules, including drugs, bilirubin, and various chaperones. 12 The protein-bound uremic toxins represented by IS are hydrophobic molecules in physical properties. The molecular weight of the β-cyclodextrin monomer is only 1135.…”

Section: Discussionmentioning

confidence: 99%

“…Its unique hydrophobic interior and hydrophilic exterior have good sorption and wrapping properties for hydrophobic substances 11 . β‐Cyclodextrin was selected as the sorbent because it can sensitively recognize and efficiently bind a variety of hydrophobic molecules, including drugs, bilirubin, and various chaperones 12 . The protein‐bound uremic toxins represented by IS are hydrophobic molecules in physical properties.…”

Section: Discussionmentioning

confidence: 99%

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Increased clearance of indoxyl sulphate in renal failure rats with the addition of water‐soluble poly‐β‐cyclodextrin to the dialysate

et al. 2021

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Aim: Indoxyl sulphate (IS), a protein-bound uremic toxin that dramatically increases in the sera of patients with end-stage renal disease (ESRD), is poorly removed by conventional haemodialysis (HD). The purpose of this study was to explore whether the addition of water-soluble sorbent poly-β-cyclodextrins (PCDs) to dialysate can increase the clearance of IS in uremic rats in vivo.Methods: Male SD rats (450-550 g, n = 18) with nephrectomy plus IS injection (10-mg/kg) were randomly divided into three groups: 1. The HD group (n = 6): conventional HD for 4 h; 2. the 2% PCD group: 2% PCD added to the dialysate, HD for 4 h; and 3. the 4% PCD group: 4% PCD added to the dialysate, HD for 4 h. The serum IS levels in model rats were similar to those of ESRD patients. A stable and safe rat HD treatment mode was established by adjusting the vascular access, blood flow rate, dialysate flow rate, dialysis pipe, dialysate configuration, temperature, treatment environment, and other aspects.Results: Our study found that adding 2% PCD to dialysate significantly improved the clearance of IS approximately twofold compared with conventional HD. Further increasing the PCD concentration to 4% did not increase IS clearance.Conclusion: Therefore, our study showed that adding water-soluble sorbent PCDs to dialysate significantly improved the clearance of IS in uremic rats in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Increased clearance of indoxyl sulphate in renal failure rats with the addition of water‐soluble poly‐β‐cyclodextrin to the dialysate

et al. 2021

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“…Several strategies have been used to remove bilirubin from the blood using a dialysis system containing adsorbents (Qiao et al 2020;Wu et al 2020;Song et al 2021). b-cyclodextrin (b-CD), a cyclic heptamer of glucose a-1, 4 D-glucopyranoside that creates a toroid structure with a hollow hydrophobic core, was adopted to remove BR from plasma (Inderbitzin et al 2010;Wang et al 2012;Liu et al 2017). These studies also demonstrated that BR inclusion complexes can be used to increase the druggability of BR.…”

Section: Introductionmentioning

confidence: 99%

Bioadhesive hydrogel comprising bilirubin/β-cyclodextrin inclusion complexes promote diabetic wound healing

Yao

Shi

Xia

et al. 2021

Pharmaceutical Biology

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Context Chronic non-healing diabetic wound therapy is an important clinical challenge. Manipulating the release of bioactive factors from an adhesive hydrogel is an effective approach to repair chronic wounds. As an endogenous antioxidant, bilirubin (BR) has been shown to promote wound healing. Nonetheless, its application is limited by its low water solubility and oxidative degradation. Objective This study developed a bilirubin-based formulation for diabetic wound healing. Materials and methods Bilirubin was incorporated into β-CD-based inclusion complex (BR/β-CD) which was then loaded into a bioadhesive hydrogel matrix (BR/β-CD/SGP). Scratch wound assays were performed to examine the in vitro pro-healing activity of BR/β-CD/SGP (25 μg/mL of BR). Wounds of diabetic or non-diabetic rats were covered with BR or BR/β-CD/SGP hydrogels (1 mg/mL of BR) and changed every day for a period of 7 or 21 days. Histological assays were conducted to evaluate the in vivo effect of BR/β-CD/SGP. Results Compared to untreated (18.7%) and BR (55.2%) groups, wound closure was more pronounced (65.0%) in BR/β-CD/SGP group. In diabetic rats, the wound length in BR/β-CD/SGP group was smaller throughout the experimental period than untreated groups. Moreover, BR/β-CD/SGP decreased TNF-α levels to 7.7% on day 3, and elevated collagen deposition and VEGF expression to 11.9- and 8.2-fold on day 14. The therapeutic effects of BR/β-CD/SGP were much better than those of the BR group. Similar observations were made in the non-diabetic model. Discussion and conclusion BR/β-CD/SGP promotes wound healing and tissue remodelling in both diabetic and non-diabetic rats, indicating an ideal wound-dressing agent.

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“…28 Researchers have found host−guest interactions between β-CD and bilirubin. In addition, there have been several kinds of adsorbents with β-CD for bilirubin removal, such as β-CDchitosan, 29 β-CD-PEI (polyethylenimine), 30 and β-CD-co-EGDMA (ethylene glycol dimethyl acrylate). 31 In addition to interacting with bilirubin, β-CD exhibits another classical host−guest interaction with adamantane (Ad).…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive Self-Assembled β-Cyclodextrin-Modified Surface for Blood Purification

Liu

Zhong

Chen

et al. 2017

ACS Biomater. Sci. Eng.

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For patients with liver failure, bilirubin (BR) is one of the endogenous toxins in their blood. Although blood purification can remove the bilirubin from the body in clinics, the detoxification system needs to be improved, and the cost needs to be decreased. In the present study, we developed a recyclable model surface that can strongly remove bilirubin. We first prepared adamantane (Ad) on a model gold surface by self-assembly. Then, we integrated the β-cyclodextrin dimer (CDD) onto the surface with host−guest interactions between one of the CD cavities in the CDD and Ad. We characterized the surface with XPS, static contact angle measurements, and AFM. In addition, we employed QCM-D to characterize the preparation process as well as the detoxification of the surface. We demonstrated that this modified surface could strongly adsorb bilirubin through host−guest interactions between the CD cavities in the CDD and bilirubin and that the detoxification was improved 1.7 times (compared to the surface only with Ad). Interestingly, after characterization with QCM-D, this surface could be recycled due to the thermoresponsive property of the host−guest interaction between the CDD and Ad. After adsorbing the toxin and increasing the temperature to 45 °C, the CDD with bilirubin could be removed from the surface. Then, the refreshed surface with CDD could be prepared again at room temperature. This cycle could be repeated at least 3 times. Additionally, during each cycle, the modified surface exhibited good detoxification to bilirubin. This modified surface also showed strong resistance to plasma proteins, decreasing the adsorption of human serum albumin (HSA) and fibrinogen (Fg). An in vitro platelet adhesion assay showed that the adhesion of the platelets on the modified surface decreased and that the platelets were in an inactivated state. The hemolysis assay showed that this surface exhibited no hemolysis activity in the samples to red blood cells (RBCs). The CCK-8 assay showed that this surface had negligible cytotoxicity to L929 cells. This work has taken advantage of the host−guest self-assembly between β-CD and BR/Ad for special recognizing adsorption, as well as the thermoresponse of β-CD-Ad inclusion for recyclable application, and these results demonstrate that this technology has great potential for removing bilirubin and decreasing clinic costs.

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Bilirubin adsorption properties of water-soluble adsorbents with different cyclodextrin cavities in plasma dialysis system

Cited by 31 publications

References 27 publications

Increased clearance of indoxyl sulphate in renal failure rats with the addition of water‐soluble poly‐β‐cyclodextrin to the dialysate

Increased clearance of indoxyl sulphate in renal failure rats with the addition of water‐soluble poly‐β‐cyclodextrin to the dialysate

Bioadhesive hydrogel comprising bilirubin/β-cyclodextrin inclusion complexes promote diabetic wound healing

Thermoresponsive Self-Assembled β-Cyclodextrin-Modified Surface for Blood Purification

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