Bimetallic chips for a surface plasmon resonance instrument

Abstract

In this work we have replaced a commonly used Au film with an Au/Ag bimetallic film working as the sensing chip in the commercial surface plasmon resonance (SPR) instrument, Biacore 3000. The bimetallic chip gives out sharper SPR dip than that by the chip made of an Au film. The applicability and stability of the bimetallic chip on the Biacore 3000 are tested by real-time monitoring of biological coupling processes between sulfamethoxazole (SMX) and anti-SMX, which is useful in rapid detection of contaminations in food products. The bimetallic chip performs stably in 6 times' usage and regeneration. Our experiment is helpful to push the bimetallic film into real applications in commercial SPR instruments.

A smartphone based surface plasmon resonance imaging (SPRi) platform for on-site biodetection

“…Thus, gold coating thickness is kept as 2 nm in order to satisfy both sensitivity and surface chemistry requirements. Apart from the previous studies which use the Ag/Au bimetallic structure to increase sensitivity [16][17][18][19][20], we used this configuration to obtain a response below 520 nm which is not possible with the commonly used single gold layered couplers.…”

“…We employed a silver/gold (Ag/Au) bilayer structure coated on the periodic corrugations of Blu-ray discs in order to perform plasmon resonance imaging at the central region of visible spectrum ( r ∼500 nm) under normal incidence illumination in aqueous environment. This allowed the optimal use of the CMOS sensor of the smartphone while maintaining high sensitivity, chemical stability and biological affinity [16][17][18][19][20]. A microfluidic channel is placed on the bimetallic layer for controlled plumbing of the liquids.…”

Micro energy dispersive x-ray fluorescence as a powerful complementary technique for the analysis of bimetallic Au/Ag/glass nanolayer composites used in surface plasmon resonance sensors

“…Gold has the advantage of high chemical resistance and is used as the upper layer. Chen et al [4] confirmed the enhanced performance of such films using a commercial Biacore instrument. Au/Ag composite layers provide a higher SNR compared to gold monolayers of the same total thickness.…”

Highly sensitive biochemical sensor comprising rectangular nanometal arrays

“…The formation of such a wave at the metal-analyte interface enables PSPR sensors to be highly sensitive to changes in the refractive index of an analyte. Therefore, PSPR sensors have been extensively applied in several fields including biology, genetic engineering, and biochemistry [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. The second category comprises localized surface plasmon resonance (LSPR) sensors that have no propagation capability.…”