Binary mixtures of diclofenac with paracetamol, ibuprofen, naproxen, and acetylsalicylic acid and these pharmaceuticals in isolated form induce oxidative stress on Hyalella azteca

Gómez-Oliván, Leobardo Manuel; Neri-Cruz, Nadia; Galár-Martínez, Marcela; Islas-Flores, Hariz; García-Medina, Sandra

doi:10.1007/s10661-014-3925-0

Cited by 39 publications

(13 citation statements)

References 54 publications

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“…In general, antagonistic interactions increased with the higher concentrations of the mixture. Similarly, Gomez-Olivan et al (2014) found that interactions between two different NSAIDs are fundamentally antagonistic in type.…”

Section: Discussionmentioning

confidence: 95%

Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca)

Stancová¹,

Plhalová²,

Blahová³

et al. 2017

Vet. Med. - Czech

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ABSTRACT:In the present study, the effects of sub-lethal sub-chronic doses of ibuprofen, diclofenac, and carbamazepine alone, and in combination (concentration range 0.02-60 µg/l), on the early life stages of tench (Tinca tinca) were investigated. The lower concentrations of pharmaceuticals tested (0.02, 0.2, 2 µg/l) represent the concentration values of these substances commonly present in surface waters or effluents from wastewater treatment plants. Multiple biomarkers of biotransformation, antioxidant defence systems, and lipid peroxidation were determined in fish after 35 days of exposure. The evaluated pharmaceuticals induced oxidative stress in fish both alone and in combination with each other. Generally, 60 µg/l of each single pharmaceutical influenced the activity of antioxidant enzymes significantly (P < 0.05), whereas the same concentration of these pharmaceuticals in combination (1 : 1 : 1) did not have any impact on the activity of these enzymes. However, changes in biotransformation and antioxidant enzymes were apparent if lower concentrations of these pharmaceuticals were administered in the mixture. Significant changes (P < 0.05) in the activities of glutathione reductase, glutathione peroxidase, and glutathione-S-transferase were observed even at environmental concentration ranges. A significant effect (P < 0.05) on lipid peroxidation levels was found only in the experimental group exposed to carbamazepine. Keywords: antioxidant defence system; lipid peroxidationList of abbreviations ANC = acid-neutralising capacity, CAT = catalase, CBZ = carbamazepine, COD Mn = chemical oxygen demand, COX = cyclooxygenase, DCF = diclofenac, DMSO = dimethylsulfoxide, GPx = glutathione peroxidase, GR = glutathione reductase, GSH = reduced active form of glutathione, GSSG = oxidised inactive form of glutathione, GST = glutathione-S-transferase, IBU = ibuprofen, LC = liquid chromatography, LC-MS/MS = liquid chromatography with tandem mass spectrometry, LPO = lipid peroxidation level, NSAIDs = non-steroidal anti-inflammatory drugs, ROS = reactive oxygen species Pharmaceutical mixtures that contaminate water sources are currently a problem worldwide. Ibuprofen (IBU), diclofenac (DCF), and carbamazepine (CBZ) are among the most frequently detected drugs in aquatic ecosystems. Their concentrations in surface water range from ng/l to tens of µg/l. Higher concentrations have been detected in developing countries due to the direct discharge of untreated wastewater from residences and hospitals into surface waters (Tran et al. 2014).

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Section: Discussionmentioning

confidence: 95%

Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca)

Stancová¹,

Plhalová²,

Blahová³

et al. 2017

Vet. Med. - Czech

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“…Also, it should be considered other contaminants not analyzed in this study. Gómez-Oliván et al (2014b) found values 72 h-LC50 to H. azteca of DCF (4.6 mg L -1 ), IBP (1.7 mg L -1 ), NPX (7.6 mg L -1 ) and PAR (7.7 mg L -1 ) in isolated systems. These results demonstrate that the studied industrial effluent is more harmful to H. azteca than isolated AINEs.…”

Section: Discussionmentioning

confidence: 86%

Oxidative stress induced in Hyalella azteca by an effluent from a NSAID-manufacturing plant in Mexico

et al. 2016

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Production in the pharmaceutical industry has increased and along with it, the amount of wastewater of various characteristics and contaminant concentrations. The main chemicals in these effluents are solvents, detergents, disinfectants-such as sodium hypochlorite (NaClO)-and pharmaceutical products, all of which are potentially ecotoxic. Therefore, this study aimed to evaluate the oxidative stress induced in the amphipod Hyalella azteca by the effluent from a nonsteroidal anti-inflammatory drug (NSAID)-manufacturing plant. The median lethal concentration (72 h-LC50) was determined and H. azteca were exposed to the lowest observed adverse effect level (0.0732 %) for 12, 24, 48 and 72 h, and biomarkers of oxidative stress were evaluated [hydroperoxide content (HPC), lipid peroxidation (LPX), protein carbonyl content (PCC), and the activity of the superoxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)]. Statistically significant increases with respect to the control group (P < 0.05) were observed in HPC, LPX and PCC in H. azteca at all exposure times. Antioxidant enzymes activity SOD, CAT and GPx activity also increased significantly (P < 0.05) with respect to the control group. In conclusion, the industrial effluent analyzed in the present study contains NSAIDs and NaClO, and induces oxidative stress in H. azteca.

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“…The inhibitory effect on early growth detected on exposure to isolated pharmaceuticals tends to be cancelled in the mixtures, which might be due to interactions between compounds or even to the plant's own metabolism. It is known that the presence of isolated pharmaceuticals in the soil can induce oxidative stress in plants and then, their antioxidant defenses can be induced (Gómez-Oliván et al, 2014), leading to different responses (phytotoxic effects) depending on the pharmaceutical, its concentration and the sensitivity of the plant species (Carvalho et al, 2014;Marsoni et al, 2014;Minden et al, 2017). For instance, IBU acts by inhibiting cyclooxygenases, enzymes that are responsible for the catalysis of the prostaglandins synthesis.…”

Section: Effect Of Pharmaceuticals Mixtures In L Sativa Seedsmentioning

confidence: 99%

Individual and mixture toxicity evaluation of three pharmaceuticals to the germination and growth of Lactuca sativa seeds

Rede

Santos

Ramos

et al. 2019

Science of The Total Environment

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HIGHLIGHTS• Amoxicillin, ibuprofen and paracetamol acute effects on Lactuca sativa were tested. • Germination and early plant growth were evaluated after a 5-day exposure. • Theexposuretosinglepharmaceuticals boosted the length of L. sativa. • Mixtures of pharmaceuticals prevent the effects of isolated compounds.This work aims to assess, individually and in mixtures, possible phytotoxic effects of three pharmaceuticals (paracetamol, ibuprofen and amoxicillin) on germination and early growth of Lactuca sativa seeds. Pharmaceuticals are an important group of emerging contaminants, whose presence has been described in several environmental compartments, including soils. However, knowledge on their possible impact in terrestrial organisms is still sparse and even more when mixtures are considered. Germination tests are important to evaluate the quality of soil and the toxic effects that contaminants can pose to plants.The acute effects of individual pharmaceuticals as well as binary and ternary mixtures were assessed using different endpoints, namely: percentage of seed germination, root elongation, shoot and leaf length, after an exposure time of five days. Overall, in the exposure of L. sativa seeds to individual pharmaceuticals there are indications of acute toxicity in the early plant growth. However, this inhibitory effect tends to be cancelled in the acute Keywords: Phytotoxicity Lactuca sativa Amoxicillin Paracetamol Ibuprofen Mixtures exposure to mixtures. This study shows the importance of evaluating the toxicity of mixtures of pharmaceuticals, since they might have distinct toxic effects when compared to the single compounds, and also because, probably, it is the closest scenario to the reality that can be found in the environment.

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Binary mixtures of diclofenac with paracetamol, ibuprofen, naproxen, and acetylsalicylic acid and these pharmaceuticals in isolated form induce oxidative stress on Hyalella azteca

Cited by 39 publications

References 54 publications

Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca)

Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca)

Oxidative stress induced in Hyalella azteca by an effluent from a NSAID-manufacturing plant in Mexico

Individual and mixture toxicity evaluation of three pharmaceuticals to the germination and growth of Lactuca sativa seeds

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