Binder-Free TiO2 Monolith-Packed Pipette Tips for the Enrichment of Phosphorylated Peptides

Lei, Chang; Zhou, Liang; Xu, Chun; Sun, Xin; Nouwens, Amanda; Yu, Chengzhong

doi:10.1071/ch16443

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…The phosphopeptide signals were less interfered with by those of the nonphosphopeptides and showed that the enrichment performance of the resultant TiO 2 -CM material was higher than that of the commercial TiO 2 material. Compared with similar materials used in MOAC and IMAC, the TiO 2 -CM material exhibited good selectivity on phosphopeptides. − This indicated that the resultant TiO 2 -CM material possessed excellent selectivity for phosphopeptide enrichment and a stronger anti-interfering ability for nonphosphopeptides than the commercial TiO 2 material.…”

Section: Results and Discussionmentioning

confidence: 98%

Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides

Zhang

Wang

Zhang

et al. 2022

ACS Biomater. Sci. Eng.

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Metal oxide affinity chromatography (MOAC) is considered to be one of the most effective methods for phosphopeptide enrichment. However, most of the materials used in the method are powder; frequent centrifugation is necessitated during the enrichment process, and potential risks of loss of peptides and materials and clogging of the column employed for liquid chromatography–mass spectrometry (LC–MS) arise. Moreover, the reusability of these materials to achieve sustainability was hardly investigated. To overcome these limitations, herein, inorganic titanium dioxide (TiO2) was coated onto the skeletal surface of the organic cellulose monolith (CM) material with a coral-like structure via a sol–gel method. This produced an organic–inorganic hybrid TiO2-CM material, which contained a combination of organic and inorganic substances, making it mimic the mollusk shell in terms of composition. The prepared TiO2-CM material as monolith exhibited excellent mechanical strength and did not break during the enrichment process; thus, the tedious implementation of multiple centrifugation cycles was prevented, thereby streamlining the experimental procedure and avoiding the loss of peptides and materials. Moreover, a large amount of TiO2 was introduced onto the surface of the CM material, and thus, the resultant TiO2-CM material exhibited a large surface area. As a result, the fabricated TiO2-CM material was successfully applied to the enrichment of phosphopeptides obtained from the tryptic digests of a BSA/β-casein (mass ratio, 500/1) mixture. The results were superior to those achieved for commercial TiO2 beads, confirming that TiO2-CM has excellent selectivity for phosphopeptides and reusability. Furthermore, 9287 unique phosphopeptides derived from the 2661 phosphoproteins were successfully identified from two milligrams of tryptic digests of Hela cell exosomes obtained through five independent replications after enriching using the TiO2-CM material. The results indicated that the material has good application prospects in the analysis of protein phosphorylation. Furthermore, TiO2-CM consists of green and cheap cellulose as the skeleton, and its synthesis process is environment-friendly, simple, and inexpensive.

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Section: Results and Discussionmentioning

confidence: 98%

Robust Dual-Biomimetic Titanium Dioxide-Cellulose Monolith for Enrichment of Phosphopeptides

Zhang

Wang

Zhang

et al. 2022

ACS Biomater. Sci. Eng.

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show abstract

“…Sun, and A. Nouwens on 'Binder-free TiO 2 Monolith Packed Pipette-Tips for the Enrichment of Phosphorylated Peptides'. [9] Denis Evans (ANU) is the 2015 winner of the David Craig Medal of the Academy of Science and has presented a research paper with co-workers Stephen Williams and Debra Bernhardt on 'A Derivation of the Gibbs Equation and the Determination of Change in Gibbs Entropy from Calorimetry'. [10] Jeffrey Reimers (UTS and Shanghai University) is the 2016 winner of the David Craig Medal of the Academy of Science and has presented an account entitled 'Putting David Craig's Legacy to Work in Nanotechnology and Biotechnology'.…”

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confidence: 99%