Binding ability of Cu2+ ions by opiate-like fragments of bovine casein

Chruscinska, Elzbieta; Dyba, Marcin; Micera, Giovanni; Ambroziak, W.; Olczak, Jacek; Zabrocki, Janusz; Kozłowski, Henryk

doi:10.1016/s0162-0134(96)00147-x

Cited by 11 publications

(11 citation statements)

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“…The mechanism of relief of inhibition is not known, but it has been speculated (6) that stretches of phosphorylated serine residues present in bovine ␣-casein may be involved. These phosphorylated regions give bovine ␣-casein a high chelating force toward positively charged metal ions (3,11,27). This chelating power may be involved in the enhancing properties of bovine ␣-casein by capturing coextracted metal ions which might otherwise act as inhibitors of both RT and PCR.…”

Section: Discussionmentioning

confidence: 99%

Highly Sensitive Assay for Detection of Enterovirus in Clinical Specimens by Reverse Transcription-PCR with an Armored RNA Internal Control

et al. 2004

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The objective of the present study was the development of a diagnostic reverse transcription (RT)-PCR for the specific detection of enterovirus (EV) RNA in clinical specimens controlled by an internal control (IC) RNA. The IC RNA contains the same primer binding sites as EV RNA but has a different probe region. The IC RNA was packaged into an MS2 phage core particle (armored) and was added to the clinical sample to allow monitoring of both extraction efficiency and RT-PCR efficiency. Serial dilutions of the IC RNA were made, and the detection limit of the RT-PCR was tested in a background of EV RNA-negative cerebrospinal fluid. The sensitivity and specificity of the RT-PCR assay were tested by using all 64 known EV serotypes, several non-EV serotypes, and two Quality Control for Molecular Diagnostics ( The human enteroviruses (EVs) are members of the family Picornaviridae, are ubiquitous, and are mainly enterically transmitted. EVs have traditionally been identified by serotype-specific antisera in a virus-neutralizing test, and 66 EV types are known to infect humans (19). The 66 EV serotypes were initially recognized and divided into five major groups: polioviruses (PV; types 1 to 3), coxsackieviruses A (CVAs; types 1 to 22 and 24), coxsackieviruses B (CVBs; types 1 to 6), echoviruses (types 1 to 7, 9, 11 to 27, and 29 to 33), and EV types 68 to 71 (17). Recent molecular analyses have proved that echovirus types 22 and 23 are genetically distinct from the members of the genus Enterovirus and have been reclassified in a separate genus, Parechovirus, in the family Picornaviridae (13,20,23).Infections with EVs cause a wide range of clinical outcomes, such as asymptomatic infections, aseptic meningitis (meningeal inflammation in the absence of a bacterial pathogen), encephalitis, paralytic poliomyelitis, and myocarditis. Although the majority of EV infections do not cause significant disease, infection can cause serious illness, especially in infants and immune-compromised patients. EV infections are the most common cause of aseptic meningitis and account for 80 to 90% of all cases of central nervous system infections for which a possible causative agent is identified (24). In the neonate, aseptic meningitis-induced complications and poor outcomes of EV infections generally occur within the first 2 days of life (1, 2). Aseptic meningitis in immune-competent adults is characterized by sudden onset of fever, but neurological abnormalities are rare, and both short-term and long-term outcomes are generally good. Encephalitis caused by EV infections is a less common but a more severe disease than aseptic meningitis (18,29,30). Immune-compromised children and adults who are infected with EV may develop chronic meningitis and encephalitis, which may last for years before becoming fatal (16).The early clinical symptoms of meningitis caused by viruses, bacteria, and fungi are quite similar and are difficult to distinguish, but the diagnosis, therapy, and outcome of disease caused by these pathogens vary considerably. A reli...

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Section: Discussionmentioning

confidence: 99%

Highly Sensitive Assay for Detection of Enterovirus in Clinical Specimens by Reverse Transcription-PCR with an Armored RNA Internal Control

et al. 2004

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“…The normal mode of coordination achieved in regular opiate-like peptides [15,16] is broken because Pro residues do not possess an ionizable peptide proton [67]. However, the presence of Pro residues promotes very stable dimeric species involving the Tyr side-chain phenolic donor.…”

Section: Tyr-d-ala-gly-phe-leu« (Cn 4 )Chmentioning

confidence: 99%

“…Therefore, the interactions between copper(II) ions and opioid peptides or their analogues were the focus of the reported work. The aim of reviewed studies was to investigate the coordinating effects of a new class of peptide chelators, tetrazole opioid peptide analogues [15][16][17][18][19][20][21][22], on copper(II) ions. The 1,5-disubstituted tetrazole ring is a cis amide bond surrogate [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

“…and (ii) what is the competitive copper binding efficiency of tetrazole peptides as compared to other opioid oligopeptides? In order to obtain the most accurate pattern of tetrazole functioning in metallopeptides, the authors investigated the chelating abilities of systems containing endogenous enkephalin Tyr-Gly-Gly-Phe-Leu [19], exogenous ␣-caseins Arg-Tyr-Leu-Gly-Tyr-Leu (casein 90-95) [15] and Arg-Tyr-LeuGly-Tyr-Leu-Glu (casein 90-96) [16], and ␤-casomorphins: bovine Tyr-Pro-Phe-Pro-Gly-Pro-Ile and human Tyr-Pro-Phe-Val-Glu-ProIle [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Tetrazole peptides as copper(II) ion chelators

Łodyga-Chruścińska¹

2011

Coordination Chemistry Reviews

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“…The interaction of Cu(II) with these biomolecules may have physiological relevance because the copper content is high especially in synaptosomal fluids which are rich in opioid peptides [4]. Studies on the Cu(II)-exorphin systems have shown that these exogenous opiate-like peptides are efficient chelating agents [5][6][7]. Moreover, the insertion of the tetrazole ring can effectively stabilize the metallopeptide structure [8][9][10].…”

Section: Introductionmentioning

confidence: 99%