Binding and release of basic fibroblast growth factor from heparinized collagen matrices

Wissink, M.J.B.; Beernink, R.; Pieper, Jeroen; Poot, Andreas A.; Engbers, G.H.M.; Beugeling, T.; Aken, W.G. van; Feijén, Jan

doi:10.1016/s0142-9612(00)00418-x

Cited by 149 publications

(102 citation statements)

References 58 publications

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“…The bFGF is released slightly more quickly from the PEG-LMWH controls than from the PEG-LMWH/PEG-PF4 ZIP hydrogels, despite the slightly higher ratio of LMWH: bFGF in the control samples. The observed burst release is similar in value to previously reported burst release in other heparinized hydrogel systems [53]; the bFGF released from those hydrogel systems has been successfully applied to stimulate cell proliferation. Hydrogel erosion kinetics were also assessed for the samples used in release experiments.…”

Section: Growth Factor Release and Hydrogel Erosionsupporting

confidence: 87%

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Zhang

Furst

Kiick

2006

Journal of Controlled Release

117

131

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The design of materials in which assembly, mechanical response, and biological properties are controlled by protein-polysaccharide interactions could provide materials that mimic the biological environment and find use in the delivery of growth factors. In the investigations reported here, a heparin-binding, coiled-coil peptide, PF4 ZIP , was employed to mediate the assembly of heparinized polymers. The heparin-binding affinity of this peptide was compared with that of other heparinbinding peptides (HBP) via heparin-sepharose chromatography and surface plasmon resonance (SPR) experiments. Results from these experiments indicate that PF4 ZIP demonstrates a higher heparin-binding affinity and heparin association rate when compared to the heparin-binding domains of antithrombin III (ATIII) and heparin-interacting protein (HIP). Viscoelastic hydrogels were formed upon the association of PF4 ZIP -functionalized star poly(ethylene glycol) (PEG-PF4 ZIP ) with low-molecular-weight heparin-functionalized star PEG (PEG-LMWH). The viscoelastic properties of the hydrogels can be altered via variations in the ratio of LMWH to PF4 ZIP . bFGF release from these gels have also been investigated. Comparison of the bFGF release profiles with the hydrogel erosion profiles indicates that bFGF delivery from this class of hydrogels is mainly an erosioncontrolled process and the rates of bFGF release can be modulated via choice of HBP or via variations in the mechanical properties of the hydrogels. Manipulation of hydrogel physical properties and erosion profiles will provide novel materials for controlled growth factor delivery and other biomedical applications.

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Section: Growth Factor Release and Hydrogel Erosionsupporting

confidence: 87%

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Zhang

Furst

Kiick

2006

Journal of Controlled Release

117

131

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“…Collagen gels are natural materials but an immune response could arise if cross species transplantation is used. These scaffolds contain sites for cell adhesion and can be covalently modified [6,[57][58][59]. Additionally, the scaffold properties can be varied by using different concentrations of collagen.…”

Section: Synthetic Materials-syntheticmentioning

confidence: 99%

Approaches to neural tissue engineering using scaffolds for drug delivery

Willerth

Sakiyama‐Elbert

2007

Advanced Drug Delivery Reviews

337

249

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This review seeks to give an overview of the current approaches to drug delivery from scaffolds for neural tissue engineering applications. The challenges presented by attempting to replicate the three types of nervous tissue (brain, spinal cord, and peripheral nerve) are summarized. Potential scaffold materials (both synthetic and natural) and target drugs are discussed with the benefits and drawbacks given. Finally, common methods of drug delivery, including degradable/diffusion-based delivery systems, affinity-based delivery systems, immobilized drug delivery systems, and electrically controlled drug delivery systems, are examined and critiqued. Based on the current body of work, suggestions for future directions of research in the field of neural tissue engineering are presented.

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“…Thus, Bentz et al [1998] covalently coupled TGF-ß to injectable collagen by means of a homobifunctional crosslinking agent, and they observed a substantial decrease of the release of the immobilized growth factor as compared to the admixed growth factor. In another approach, Wissink et al [2001b] made use of the heparin binding affinity of bFGF for physically binding this growth factor to heparin covalently incorporated into collagen films [Wissink et al, 2001a]. These authors were able to show that the heparinization of the cross-linked collagen has a stimulative effect on the proliferation of endothelial cells [Wissink et al, 2000b].…”

Section: Introductionmentioning

confidence: 99%

Modification of Collagen Matrices for Enhancing Angiogenesis

Yao

Prével

Koch³

et al. 2004

Cells Tissues Organs

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The vascularization of engineered tissues in many cases does not keep up with the ingrowth of cells. Nutrient and oxygen supply are not sufficient, which ultimately leads to the death of the invading cells. The enhancement of the angiogenic capabilities of engineered tissues therefore represents a major challenge in the field of tissue engineering. The immobilization of angiogenic growth factors may be useful for enhancing angiogenesis. The most potent angiogenic growth factor specific to endothelial cells, vascular endothelial growth factor (VEGF), occurs in several splice variants. The variant with 165 amino acids both has a high angiogenic activity and a high affinity for heparin. We therefore incorporated heparin molecules into collagen matrices by covalently cross-linking them to amino functions on the collagen. Physical binding of VEGF to the heparin may then prevent a rapid clearance from the implant, while the release rate may become coupled to the degradation of the collagen matrix. The modified matrices were characterized by determination of the extent of the heparin immobilization, the in vitro degradation rate by collagenase. For testing the angiogenic properties, non-modified and heparinized collagen specimens were – either loaded with VEGF or non-loaded – subcutaneously implanted on the back of rats. Specimens were explanted after varying periods of implantation, the dry weights and the hemoglobin contents, as well as immunostained histological sections were evaluated: heparinized collagen matrices loaded with VEGF are vascularized to a substantially higher extent as compared to non-modified matrices.

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Binding and release of basic fibroblast growth factor from heparinized collagen matrices

Cited by 149 publications

References 58 publications

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Approaches to neural tissue engineering using scaffolds for drug delivery

Modification of Collagen Matrices for Enhancing Angiogenesis

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