Binding between lead ions and the high-abundance serum proteins

Guo, Ming; He, Ling; Strong, Peter James; Wang, Hailong

doi:10.1016/j.chemosphere.2014.05.018

Cited by 11 publications

(9 citation statements)

References 58 publications

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“…Albumin is the most abundant plasma protein, which serves to buffer the blood, maintain the osmotic pressure, and as a carrier of many compounds (Guo et al 2014). Because of their ROS scavenging activity, thiol groups of cysteine residues of albumin determine the plasma redox status (Dobrakowski et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Association between subchronic and chronic lead exposure and levels of antioxidants and chemokines

Dobrakowski

Kasperczyk

Pawlas

et al. 2016

Int Arch Occup Environ Health

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PurposeThis study aimed to compare the influence of lead on the non-enzymatic antioxidant defenses and the levels of chemokines in workers subchronically and chronically exposed to lead.MethodsThe study population was divided into three groups. The first group consisted of male workers subchronically exposed to lead for 40 ± 3.2 days, while the second group included male workers chronically exposed to lead. The third group was a control group.ResultsThe levels of uric acid and bilirubin were significantly higher after a subchronic exposure to lead compared to the baseline by 22 and 35 %, respectively. Similarly, the values of total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) increased by 15, 50, and 33 %, respectively. At the same time, the levels of thiol groups and albumin decreased by 5 and 8 %, respectively. Additionally, the levels of interleukin-8 (IL-8) and macrophage inflammatory protein-1β (MIP-1β) were significantly higher after a subchronic exposure to lead compared to the baseline by 34 and 20 %, respectively. Moreover, IL-8 level was significantly higher by 40 % in the group of workers chronically exposed to lead than in the control group, while the level of interferon gamma-induced protein-10 (IP-10) was significantly lower by 28 %.ConclusionsSimilar to chronic lead exposure, subchronic exposure to lead is associated with elevated blood levels of uric acid and bilirubin in humans. This probably results in increased TAC value despite thiol depletion. However, the compensatory activation of non-enzymatic antioxidant defenses seems to be insufficient to protect against lead-induced oxidative stress, which may be additively enhanced by the pro-inflammatory action of chemokines, especially IL-8.

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Section: Discussionmentioning

confidence: 99%

Association between subchronic and chronic lead exposure and levels of antioxidants and chemokines

Dobrakowski

Kasperczyk

Pawlas

et al. 2016

Int Arch Occup Environ Health

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“…When BSA-E2 was bonded onto Cu 2 S, it was obvious that the spectrum of Cu 2 S-BSA-E2 bioconjugates (curve c) was a superposition of curve a and curve b. Electrochemical methods are also suitable for investigating the interaction between biological molecules and other materials. 25 Due to the proteins are intrinsically unable to act as redox partners, the combined biological molecules will weaken the electrochemical signals obviously. From ESI Fig.…”

Section: Characterization Of Cu 2 S and Cu 2 S-bsa-e2 Bioconjugatesmentioning

confidence: 99%

An ultrasensitive electrochemical immunosensor for determination of estradiol using coralloid Cu₂S nanostructures as labels

et al. 2015

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Herein, coralloid Cu 2 S nanoparticles were prepared through a facile etching method and used as labels for the first time to fabricate an electrochemical immunosensor. A novel competitive immunoassay was then proposed using Cu 2 S covalent conjugation with bovine serum albumin (BSA)-estradiol (E2) for the sensitive detection of trace E2 concentrations. Without using enzyme-label and acid dissolution, the Cu 2 S can generate excellent electrochemical signals. The quantitative detection was based on the competitive binding of the E2 antibody with Cu 2 S-labeled E2 or free E2. The redox signal decreased with increasing concentration of the free E2 as the amount of Cu 2 S-BSA-E2 labels decreased at the immunosensor probe. Cyclic voltammetry and electrochemical impedance spectroscopy techniques were used to characterize the immunosensor. Square wave voltammetry was used to monitor the electrochemical response. The immunosensor exhibited a wide linear response from 25 to 7500 pg mL À1 with a detection limit of 7.5 pg mL À1 . The proposed method showed good precision, broad linear range, and acceptable stability and could be used for the detection of E2 in real samples, which showed promising application in field research.

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“…In metal coordination chemistry, the term complex means a central metal atom or ions surrounded by a set of ligands, a large number of molecules and ions can behave as ligands, and a large number of metals ions form complexes [6]. A number of functional groups participate in metal binding in metalloproteins; the side chains of Glu , Tyr, Cys, His Arg, Lys, Asp and Met and although free cysteines can potentially bind chelated metals, in practice they are rarely available in the appropriate reduced state [7].In new dimension of protein separations, the apparent affinity of a protein for a metal chelate depends strongly on the metal ion involved in coordination. Protein retention on different metal mirrors the affinity of the metal for imidazole.…”

Section: Introductionmentioning

confidence: 99%

“…Protein retention on different metal mirrors the affinity of the metal for imidazole. The stability constant for complexation with imidazole follows the order Cu 2+ > Zn 2+ and also depends on the quantity of protein that can be loaded onto a given metal-chelate support [7]. Immobilized metal ion chromatography (IMAC) was used to purify protein by using metal binding concept, Ueda et al, (2003).…”

Section: Introductionmentioning

confidence: 99%

Metal Selectivity of Hevea Protein Isolated from Natural Rubber Latex Skim Serum

Tahir¹,

Misran²,

Othman³

et al. 2019

IJET

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Hevea protein isolated from skim serum, a by-product of centrifugation process, contains useful proteins in extracting metal. It can be used directly from the source or purified before reacting with metal solutions. Those proteins bind with metal at varying degrees. Upon exposure from as low as 2 ppm concentration to up to 20 ppm metal solution concentration, different binding characteristics were seen. The reasons of such inconsistency in the characteristics might be due to the existence of some of the metal itself in the NRL serum. Mg++ and Zn++ are common metal found in NRL products and those metals would show the slightest in binding with hevea protein. Other metals which were covered in this scope of study shows a good binding characteristics disregard of the group of metals belongs. Selectivity was measured from the final concentration of metal in percentage. In most cases, lead, copper and cadmium show good interaction with hevea proteins.

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Binding between lead ions and the high-abundance serum proteins

Cited by 11 publications

References 58 publications

Association between subchronic and chronic lead exposure and levels of antioxidants and chemokines

Association between subchronic and chronic lead exposure and levels of antioxidants and chemokines

An ultrasensitive electrochemical immunosensor for determination of estradiol using coralloid Cu₂S nanostructures as labels

Metal Selectivity of Hevea Protein Isolated from Natural Rubber Latex Skim Serum

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Binding between lead ions and the high-abundance serum proteins

Cited by 11 publications

References 58 publications

Association between subchronic and chronic lead exposure and levels of antioxidants and chemokines

Association between subchronic and chronic lead exposure and levels of antioxidants and chemokines

An ultrasensitive electrochemical immunosensor for determination of estradiol using coralloid Cu2S nanostructures as labels

Metal Selectivity of Hevea Protein Isolated from Natural Rubber Latex Skim Serum

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An ultrasensitive electrochemical immunosensor for determination of estradiol using coralloid Cu₂S nanostructures as labels