2000
DOI: 10.1016/s0014-2999(00)00411-8
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Binding domain of oligomycin on Na+,K+-ATPase

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Cited by 15 publications
(10 citation statements)
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“…This ATPase activity was still slightly oligomycin sensitive, suggesting that some of the 20S and 40S intact complexes remained in the mitochondria after three days of RNAi induction, consistent with the results from the Western analyses (Figure 5B). It should be noted that oligomycin in high concentrations also affects the activity of mitochondrial F 1 and Na +/ K + ATPases [45],[46]. Treatment with azide decreased the amount of the free phosphate down to 45±6.3 and 46±6.8 nmol, respectively, which is comparable to the levels seen in non-induced oligomycin- or azide-treated cells (Figure 7).…”
Section: Resultssupporting
confidence: 56%
“…This ATPase activity was still slightly oligomycin sensitive, suggesting that some of the 20S and 40S intact complexes remained in the mitochondria after three days of RNAi induction, consistent with the results from the Western analyses (Figure 5B). It should be noted that oligomycin in high concentrations also affects the activity of mitochondrial F 1 and Na +/ K + ATPases [45],[46]. Treatment with azide decreased the amount of the free phosphate down to 45±6.3 and 46±6.8 nmol, respectively, which is comparable to the levels seen in non-induced oligomycin- or azide-treated cells (Figure 7).…”
Section: Resultssupporting
confidence: 56%
“…However, the binding site for this compound is located within the membrane spanning F 0 component [57]. In addition to the mitochondrial F 1 F 0 -ATPase, oligomycin inhibits the Na + /K + - and H + /K + -ATPases [58, 59], suggesting that these enzymes contain similar binding pockets. In contrast to Bz-423, oligomycin induces time-dependent inhibition of the F 1 F 0 -ATPase [60], and is associated with decreases in total cellular ATP [52].…”
Section: Discussionmentioning
confidence: 99%
“…7A,C) increase in rhodamine-123 fluorescence. At elevated concentrations, oligomycin is also a potent inhibitor of the plasmalemmal Na + /K + -ATPase (Homareda et al, 2000). To show that the oligomycinmediated rise in rhodamine-123 fluorescence is not an artifactual side effect of destabilizing Na + and K + gradients, we applied 1 mmol l −1 ouabain following the anoxia-mediated rise in rhodamine-123 fluorescence.…”
Section: Uncoupling the Mitochondria With A Proton Ionophore Followinmentioning
confidence: 99%