Binding‐Induced DNA Nanomachines Triggered by Proteins and Nucleic Acids

Zhang, Hongquan; Lai, Maode; Zuehlke, Albert; Peng, Hanyong; Li, Xing‐Fang; Le, X. Chris

doi:10.1002/ange.201506312

Cited by 40 publications

(29 citation statements)

References 35 publications

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“…The construction of protein-responsive nucleic acid nanodevices in vitro has been reported. Examples of such nanodevices include nanomechanical DNA origami that detects proteins at the single-molecule level 8 , DNA nanorobots that recognize the cell surface and control cell signalling by utilizing the interaction between DNA aptamers and protein 10 , and protein-triggered DNA nanomachines on gold nanoparticles that control the assembly and cleavage of DNA components 50 . However, the detection of proteins by DNA nanodevices has been limited to in vitro or cell surfaces.…”

Section: Discussionmentioning

confidence: 99%

Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate

et al. 2017

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Nucleic acid nanotechnology has great potential for future therapeutic applications. However, the construction of nanostructured devices that control cell fate by detecting and amplifying protein signals has remained a challenge. Here we design and build protein-driven RNA-nanostructured devices that actuate in vitro by RNA-binding-protein-inducible conformational change and regulate mammalian cell fate by RNA–protein interaction-mediated protein assembly. The conformation and function of the RNA nanostructures are dynamically controlled by RNA-binding protein signals. The protein-responsive RNA nanodevices are constructed inside cells using RNA-only delivery, which may provide a safe tool for building functional RNA–protein nanostructures. Moreover, the designed RNA scaffolds that control the assembly and oligomerization of apoptosis-regulatory proteins on a nanometre scale selectively kill target cells via specific RNA–protein interactions. These findings suggest that synthetic RNA nanodevices could function as molecular robots that detect signals and localize target proteins, induce RNA conformational changes, and programme mammalian cellular behaviour.

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Section: Discussionmentioning

confidence: 99%

Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate

et al. 2017

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“…DNA origami was further used to construct two-dimensional tracks, increasing the walking steps and directionality of the motor 26 27 28 . Recently, DNA tracks were built on nano- and micro-materials, including gold film 7 , microparticles 8 , carbon nanotubes 10 29 and gold nanoparticles (AuNPs) 30 31 , further improving the mobility and processivity of the DNA motors. Instructional strands were included precisely at specific positions of DNA tracks, enabling control of the walking direction of the motors 22 32 .…”

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confidence: 99%

A microRNA-initiated DNAzyme motor operating in living cells

et al. 2017

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Synthetic DNA motors have great potential to mimic natural protein motors in cells but the operation of synthetic DNA motors in living cells remains challenging and has not been demonstrated. Here we report a DNAzyme motor that operates in living cells in response to a specific intracellular target. The whole motor system is constructed on a 20 nm gold nanoparticle (AuNP) decorated with hundreds of substrate strands serving as DNA tracks and dozens of DNAzyme molecules each silenced by a locking strand. Intracellular interaction of a target molecule with the motor system initiates the autonomous walking of the motor on the AuNP. An example DNAzyme motor responsive to a specific microRNA enables amplified detection of the specific microRNA in individual cancer cells. Activated by specific intracellular targets, these self-powered DNAzyme motors will have diverse applications in the control and modulation of biological functions.

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“…This technique makes use of the attractive properties of nucleic acids for desired signal transduction and amplification [81,82]. For example, Landegren et al [83] combined proximity ligation assay (PLA) using DNA-bound antibodies to recognize the target molecule with RCA amplification to develop a method for highly sensitive protein detection.…”

Section: Proximity Ligation For Amplified Immunoassaymentioning

confidence: 99%

Signal Amplification for Highly Sensitive Immunosensing

2017

J. Anal. Test.

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To dissolve the bottleneck problem of life and biomedical science in detection of biomolecules with low abundance and acquisition of ultraweak biological signals, highly sensitive analytical methods coupling with the specificity of biological recognition events have been quickly developed by the exploring of signal amplification strategies. These strategies have extensively been introduced into the development of highly sensitive immunosensing methods by combining with highly specific immunological recognition. They can be divided into two groups. One group of strategies attempts to transfer the immunological recognition event into large number of reporter probes or signal probes for signal readout by employing nano/micro-materials as vehicles for multi-labeling and/or molecular biological amplification for increasing the abundance of the signal molecules. The other uses nanomaterials or enzyme mimics as catalytic tools/tags to obtain enhanced detection signal. This review focuses on the significant advances in design of signal amplification strategies for highly sensitive immunosensing.

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Binding‐Induced DNA Nanomachines Triggered by Proteins and Nucleic Acids

Cited by 40 publications

References 35 publications

Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate

Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate

A microRNA-initiated DNAzyme motor operating in living cells

Signal Amplification for Highly Sensitive Immunosensing

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