Binding of 14-3-3κ to ADF4 is involved in the regulation of hypocotyl growth and response to osmotic stress in Arabidopsis

Yao, Huan; Li, Xiaohua; Lü, Peng; Hua, Xinyue; Zhang, Qian; Li, Kexuan; Huang, Yaling; Ji, Hao; Wu, Xiaoqing; Chen, Yihong; Yang, Yi; Wang, Jianmei

doi:10.1016/j.plantsci.2022.111261

Cited by 10 publications

(15 citation statements)

References 38 publications

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“…Plant materials and growth conditions Arabidopsis plants used in this study were all in the Columbia (Col-0) background. The cark3-4 and adf4 T-DNA insertion mutants, OE- ADF4 #10, #17, #18 and CARK3 -OE 2 overexpression plants were previously described ( Wang et al., 2020 ; Yao et al., 2022 ). Plants were grown on soil-vermiculite mixtures at 22°C under 60% relative humidity with cycles of 16 h light and 8 h dark.…”

Section: Methodsmentioning

confidence: 99%

“…The ADF4-YFP N and CARK3-YFP C constructs were previously described ( Wang et al., 2020 ; Yao et al., 2022 ). The constructs were transformed into Agrobacteria GV3101 stain.…”

Section: Methodsmentioning

confidence: 99%

“…PRF4 and PRF5, as actin-binding proteins, could regulate vesicle trafficking and polarity establishment during pollen germination by enhancing the interaction between AtFH5 (Arabidopsis formin homology 5) and actin filaments ( Liu et al., 2021 ). In the dark, ADF4 mutants have longer hypocotyl and epidermal cells when compared with wild-type seedlings ( Henty et al., 2011 ; Yao et al., 2022 ). However, the molecular mechanism of ADF4 is not uncovered in regulating the hypocotyl elongation.…”

Section: Introductionmentioning

confidence: 99%

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CARK3-mediated ADF4 regulates hypocotyl elongation and soil drought stress in Arabidopsis

Lü

Yao

et al. 2022

Front. Plant Sci.

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Actin depolymerization factors (ADFs), as actin-binding proteins, act a crucial role in plant development and growth, as well as in response to abiotic and biotic stresses. Here, we found that CARK3 plays a role in regulating hypocotyl development and links a cross-talk between actin filament and drought stress through interaction with ADF4. By using bimolecular fluorescence complementation (BiFC) and GST pull-down, we confirmed that CARK3 interacts with ADF4 in vivo and in vitro. Next, we generated and characterized double mutant adf4cark3-4 and OE-ADF4:cark3-4. The hypocotyl elongation assay indicated that the cark3-4 mutant seedlings were slightly longer hypocotyls when compared with the wild type plants (WT), while CARK3 overexpressing seedlings had no difference with WT. In addition, overexpression of ADF4 significantly inhibited long hypocotyls of cark3-4 mutants. Surprisingly, we found that overexpression of ADF4 markedly enhance drought resistance in soil when compared with WT. On the other hand, drought tolerance analysis showed that overexpression of CARK3 could rescue adf4 drought susceptibility. Taken together, our results suggest that CARK3 acts as a regulator in hypocotyl elongation and drought tolerance likely via regulating ADF4 phosphorylation.

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Section: Methodsmentioning

confidence: 99%

“…The ADF4-YFP N and CARK3-YFP C constructs were previously described ( Wang et al., 2020 ; Yao et al., 2022 ). The constructs were transformed into Agrobacteria GV3101 stain.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CARK3-mediated ADF4 regulates hypocotyl elongation and soil drought stress in Arabidopsis

Lü

Yao

et al. 2022

Front. Plant Sci.

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“…Actin‐bundling villin1 (VLN1) aids in root hair development during osmotic stress through GLABRA2‐mediated VLN1 pathway that regulates actin dynamics and thereby modulates cell tip growth (Wang, Bi, et al, 2020). Similarly, actin‐depolymerizing factor (ADF4) promotes osmotic stress tolerance by interacting with 14‐3‐3κ which prevents association of ADF4 with actin filaments and actin depolymerization (Yao et al, 2022). Furthermore, both microtubules and actin filaments have their associated proteins which are functionally defined as potential cytoskeletal candidates and are involved in two‐prong organization of cytoskeleton under hypersalinity (Komati et al, 2005; Lee et al, 2001; Wang et al, 2011).…”

Section: Plant Cytoskeleton Dynamics and Adaptation To Hypersalinity ...mentioning

confidence: 99%

The plant cytoskeleton takes center stage in abiotic stress responses and resilience

Theboral

Yunus

Chakraborty

et al. 2022

Plant Cell & Environment

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Stress resilience behaviours in plants are defensive mechanisms that develop under adverse environmental conditions to promote growth, development and yield. Over the past decades, improving stress resilience, especially in crop species, has been a focus of intense research for global food security and economic growth. Plants have evolved specific mechanisms to sense external stress and transmit information to the cell interior and generate appropriate responses. Plant cytoskeleton, comprising microtubules and actin filaments, takes a center stage in stress‐induced signalling pathways, either as a direct target or as a signal transducer. In the past few years, it has become apparent that the function of the plant cytoskeleton and other associated proteins are not merely limited to elementary processes of cell growth and proliferation, but they also function in stress response and resilience. This review summarizes recent advances in the role of plant cytoskeleton and associated proteins in abiotic stress management. We provide a thorough overview of the mechanisms that plant cells employ to withstand different abiotic stimuli such as hypersalinity, dehydration, high temperature and cold, among others. We also discuss the crucial role of the plant cytoskeleton in organellar positioning under the influence of high light intensity.

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“…In Arabidopsis , most proteins identified in protein synthesis during seed development were ribosomal-related and could interact with the 14-3-3 isoforms χ and ε [ 16 ]. Additionally, studies have shown that 14-3-3 proteins could bind ADF4 (one protein of the actin-depolymerizing factor family) in regulating hypocotyl growth [ 19 ]. These results indicated that 14-3-3 proteins functioned together with other proteins during plants growth and development.…”

Section: Introductionmentioning

confidence: 99%

Identification of the 14-3-3 Gene Family in Bamboo and Characterization of Pe14-3-3b Reveals Its Potential Role in Promoting Growth

Guo

Zhu

Yang

et al. 2022

IJMS

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The 14-3-3 protein family plays an important role in regulating plant growth and development. The genes of the 14-3-3 family have been reported in multiple species. However, little is known about the 14-3-3 gene family in bamboo. In this study, a total of 58 genes belonging to the 14-3-3 family were identified in three representative bamboo species, i.e., Olyra latifolia, Phyllostachys edulis, and Bonia amplexicaulis, whose encoding proteins were grouped into ε and non-ε groups by phylogeny analysis with 14-3-3 proteins from Arabidopsis thaliana and Oryza sativa. The 14-3-3s had diverse gene structures and motif characteristics among the three bamboo species. Collinearity analysis suggested that the genes of the 14-3-3 family in bamboo had undergone a strong purification selection during evolution. Tissue-specific expression analysis showed the expression of Pe14-3-3s varied in different tissues of P. edulis, suggesting that they had functional diversity during growth and development. Co-expression analysis showed that four Pe14-3-3s co-expressed positively with eight ribosomal genes. Yeast two-hybrid (Y2H) assays showed that Pe14-3-3b/d could interact with Pe_ribosome-1/5/6, and qPCR results demonstrated that Pe14-3-3b/d and Pe_ribosome-1/5/6 had similar expression trends with the increase in shoot height, which further confirmed that they would work together to participate in the shoot growth and development of bamboo. Additionally, the transgenic Arabidopsis plants overexpressing Pe14-3-3b had longer roots, a larger stem diameter, an earlier bolting time and a faster growth rate than wild-type Arabidopsis, indicating that Pe14-3-3b acted as a growth promoter. Our results provide comprehensive information on 14-3-3 genes in bamboo and highlight Pe14-3-3b as a potential target for bamboo improvement.

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Binding of 14-3-3κ to ADF4 is involved in the regulation of hypocotyl growth and response to osmotic stress in Arabidopsis

Cited by 10 publications

References 38 publications

CARK3-mediated ADF4 regulates hypocotyl elongation and soil drought stress in Arabidopsis

CARK3-mediated ADF4 regulates hypocotyl elongation and soil drought stress in Arabidopsis

The plant cytoskeleton takes center stage in abiotic stress responses and resilience

Identification of the 14-3-3 Gene Family in Bamboo and Characterization of Pe14-3-3b Reveals Its Potential Role in Promoting Growth

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