Binding of [3H]pifluthixol, a dopamine antagonist, in the brain of the American cockroach, Periplaneta americana

Notman, H.J.; Downer, Roger

doi:10.1016/0020-1790(87)90058-8

Cited by 15 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4C). Flupenthixol, an antagonist of dopamine receptors in the cockroach brain (Notman and Downer, 1987;Orr et al, 1992), greatly reduced venom-induced grooming when injected into the cockroach's hemolymph prior to the wasp sting ( Fig 4D). In contrast, mianserin, which has been found to be an effective octopamine receptor antagonist in locust brain and cockroach nerve cord (Roeder, 1992;Orr et al, 1992), did not reduce venom-induced grooming at all (Fig.…”

Section: Wasp Venom Injection Induces Groomingmentioning

confidence: 98%

Parasitoid wasp uses a venom cocktail injected into the brain to manipulate the behavior and metabolism of its cockroach prey

Gal¹,

Rosenberg²,

Libersat³

2005

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Unlike other venomous predators, the parasitoid wasp Ampulex compressa incapacitates its prey, the cockroach Periplaneta americana, to provide a fresh food supply for its offspring. We first established that the wasp larval development, from egg laying to pupation, lasts about 8 days during which the cockroach must remain alive but immobile. To this end, the wasp injects a cocktail of neurotoxins to manipulate the behavior of the cockroach. The cocktail is injected directly into the head ganglia using biosensors located on the stinger. The head sting induces first 30 min of intense grooming followed by hypokinesia during which the cockroach is unable to generate an escape response. In addition, stung cockroaches survive longer, lose less water, and consume less oxygen. Dopamine contained in the venom appears to be responsible for inducing grooming behavior. For the hypokinesia, our hypothesis is that the injected venom affects neurons located in the head ganglia, which send descending tonic input to bioaminergic neurons. These, in turn, control the thoracic premotor circuitry for locomotion. We show that the activity of identified octopaminergic neurons from the thoracic ganglia is altered in stung animals. The alteration in the octopaminergic neurons' activity could be one of the mechanisms by which the venom modulates the escape circuit in the cockroach's central nervous system and metabolism in the peripheral system.

show abstract

Section: Wasp Venom Injection Induces Groomingmentioning

confidence: 98%

Parasitoid wasp uses a venom cocktail injected into the brain to manipulate the behavior and metabolism of its cockroach prey

Gal¹,

Rosenberg²,

Libersat³

2005

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

show abstract

“…In the present study, we show that EEDQ likewise suppresses venom-evoked grooming behavior. Moreover, typical venom-evoked grooming behavior is restored once DARs are protected by the reversible broad spectrum DAR antagonist flupenthixol injected prior to EEDQ ( Notman and Downer, 1987 ; Hess et al, 1988 ).…”

Section: Discussionmentioning

confidence: 99%

“…Cis-(Z)-flupenthixol dihydrochloride (Sigma), a reversible antagonist of D1 and D2 dopamine receptors ( Notman and Downer, 1987 ) was injected (18 nl, 0.01 mol l −1 ) into the CX immediately prior to EEDQ injection (18 nl EEDQ 0.01 mmol l −1 ). Cockroach behavior was evaluated 6 h after injection ( n =12).…”

Section: Methodsmentioning

confidence: 99%

Parasitoid wasp venom manipulates host innate behavior via subtype-specific dopamine receptor activation

Nordio

Kaiser

Adams

et al. 2022

Journal of Experimental Biology

View full text Add to dashboard Cite

The subjugation strategy employed by the jewel wasp is unique in that it manipulates the behavior of its host, the American cockroach, rather than inducing outright paralysis. Upon envenomation directly into the central complex (CX), a command center in the brain for motor behavior, the stung cockroach initially engages in intense grooming behavior, then falls into a lethargic sleep-like state referred to as hypokinesia. Behavioral changes evoked by the sting are due at least in part to the presence of the neurotransmitter dopamine in the venom. In insects, dopamine receptors are classified as two families, the D1-like and the D2-like receptors. However, specific roles played by dopamine receptor subtypes in venom-induced behavioral manipulation by the jewel wasp remain largely unknown. In the present study, we used a pharmacological approach to investigate roles of D1-like and D2-like receptors in behaviors exhibited by stung cockroaches, focusing on grooming. Specifically, we assessed behavioral outcomes of focal CX injections of dopamine receptor agonists and antagonists. Both specific and non-specific compounds were used. Our results strongly implicate D1-like dopamine receptors in venom-induced grooming. Regarding induction of hypokinesia, our findings demonstrate that dopamine signaling is necessary for induction of long-lasting hypokinesia caused by brain envenomation.

show abstract

Distribution of dopamine receptors and dopamine receptor homologs in the brain of the honey bee,Apis mellifera L.

Kokay

Ebert

Kirchhof

et al. 1999

Microsc. Res. Tech.

View full text Add to dashboard Cite

Binding of [3H]pifluthixol, a dopamine antagonist, in the brain of the American cockroach, Periplaneta americana

Cited by 15 publications

References 17 publications

Parasitoid wasp uses a venom cocktail injected into the brain to manipulate the behavior and metabolism of its cockroach prey

Parasitoid wasp uses a venom cocktail injected into the brain to manipulate the behavior and metabolism of its cockroach prey

Parasitoid wasp venom manipulates host innate behavior via subtype-specific dopamine receptor activation

Distribution of dopamine receptors and dopamine receptor homologs in the brain of the honey bee,Apis mellifera L.

Contact Info

Product

Resources

About