Binding of a cationic phenazinium dye in anionic liposomal membrane: a spectacular modification in the photophysics

Binding interaction of differently charged fluorescent probes with egg yolk phosphatidylcholine and the effect of β-cyclodextrin on the lipid–probe complexes: A fluorometric investigation

“…We have monitored the fluorescence behavior of the three probes in water-dioxane mixtures of varying composition to determine the polarities around the probes in different microenvironments. Choice of water/dioxane mixture over water/alcohol is emphasized from the fact that the former covers a wider range of polarity [48,49].…”

“…Local polarity around a probe in biomimetic microheterogeneous environments can be estimated from a comparison of the spectral properties of the fluorophore in the environment with those of the probe in pure solvents or solvent mixtures of known polarities [48,49]. In the present study we have endeavored to estimate the micropolarities of the different environments around the fluorophores keeping in mind the fact that there are only few reports on the prospective use of polarity sensitive fluorophore molecules for the determination of micropolarity in the context of the present study.…”

Nanocapsules for 5-fluorouracil delivery decorated with a poly(2-ethylhexyl methacrylate-co-7-(4-trifluoromethyl)coumarin acrylamide) cross-linked wall

“…The response of the probe's (PhS, LY) emission to changes in its environment after photopolymerisation was determined and compared with the emission in water (different viscosities and polarities induce solvatochromism). 70 Determination of the in vitro release of encapsulated 5-FU PhS dissolved in water indicated the maximum fluorescence emission l Em = 582 nm (l Ex = 510 nm) (Fig.…”

Thermodynamic investigations of ligand–protein interactions: Binding of the phenazinium dyes phenosafranin and safranin O with human serum albumin

“…PSF is used as an inhibitor of ribonucleotide reductase whose key role in DNA synthesis and control in cell growth has made this an important molecule for anticancer therapy [16]. Both PSF and SO are used as bio-probes for determination of hyaluronic acid, inhibition of human ribonucleotide reductase, detection of double stranded DNA, molecular adhesive for protein immobilization, analysing membrane organization and heterogeneity, and in several other applications [17][18][19][20][21][22][23]. Both dyes bind strongly to DNA by intercalation exhibiting remarkable base sequence specificity making them potential candidates as DNA targeted therapeutic agents [24][25][26].…”