Binding of a surface protein of Staphylococcus aureus to cultured ovine mammary gland epithelial cells

Aguilar, Begoña; Iturralde, Marı́a

doi:10.1016/s0378-1135(01)00383-2

Cited by 10 publications

(10 citation statements)

References 26 publications

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Section: Introductionmentioning

confidence: 97%

“…). This reduced antimicrobial susceptibility is attributed to a decrease in the diffusion of the drug through the biofilm matrix and in the metabolic activity of bacteria inside the matrix (Aguilar & Iturralde ).…”

Section: Introductionmentioning

confidence: 97%

“…Bovine mastitis caused by Staphylococcus aureus is a frequently chronic disease that is difficult to treat. Some S. aureus strains produce a variety of virulence factors such as toxins, surface proteins, coagulase and biofilms (Waldvogel 1995;Aguilar & Iturralde 2001). The difficulty in treating intramammary infections with S. aureus is related to biofilm formation (Melchior et al 2006), which significantly increases the capacity of producer strains to adhere and colonize the epithelial surface of the mammary gland when compared to non-producer variants.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis

Castelani

Pilon

Martins

et al. 2014

Animal Science Journal

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Biofilm formation and antimicrobial resistance of Staphylococcus aureus are important virulence factors in cases of mastitis in dairy cows. However, few studies have investigated mastitis strains isolated from heifers. Within this context, the objective of the present study was to investigate biofilm formation on Congo red agar, the presence of the icaA and icaD genes by polymerase chain reaction (PCR), and the percentage of in vitro antimicrobial resistance of 110 S. aureus isolates from mammary gland secretions of heifers and cows with mastitis. PCR detected the icaA and icaD genes in 98% and 100% of isolates, respectively. However, only 55.5% of all isolates produced a biofilm on Congo red agar. Antimicrobial susceptibility testing revealed that 47.0% of isolates from heifers and 70.4% of isolates from cows were resistant to at least one of the antimicrobial agents tested. Resistance to penicillin and/or ampicillin was the most frequent (44.5%). These results indicate the need to implement prophylactic and control measures of mastitis for heifers. Heifers and cows can carry resistant strains with the capacity of biofilm production, a fact representing a threat to public health and animal well-being and generating losses to dairy farmers.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis

Castelani

Pilon

Martins

et al. 2014

Animal Science Journal

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“…This layer allows the pathogen to adhere to and colonise the host mammary gland epithelium (Baselga et al , 1993; Bergey & Holt, 1994; Aguilar & Iturralde, 2001). …”

Section: Introductionmentioning

confidence: 99%

Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis

Melo

Ferreira

Filho

et al. 2013

Braz. J. Microbiol.

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Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well “U” bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production.

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“…aureus ("S. aureus") είναι ο πιο σημαντικός αιτιολογικός παράγοντας κλινικής μαστίτιδας σε αρμεγόμενες προβατίνες (Aguilar & Iturralde 2001. Απομονώνεται επίσης από περιστατικά μαστίτιδας σε προβατίνες κρεατοτταραγωγικής κατεύθυνσης (Jones JET 1985.…”

Section: σταφυλόκοκκοιunclassified

Προστατευτικός ρόλος της θηλής και σημασία των παθήσεων της στην εκδήλωση μαστίτιδας από mannheimia haemolytica σε πρόβατα

Μαυρογιάννη¹

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The objectives of the present thesis were: (i) the study of the dynamics of infection of bacteria in the teat of ewes, (ii) the elucidation of the role of the teat as a protective structure for the mammary gland of ewes and (iii) the evaluation of the importance of the disorders of the teats in predisposing ewes to mastitis.The thesis is divided into five chapters followed by the General Discussion.In the first Chapter, the relevant literature is reviewed. The Chapter is subdivided into four Parts. In Part A the literature on the anatomy and histology of the teat of ewes is briefly reviewed. In Part Β the literature on ovine mastitis is presented. In Part C the literature on the defence mechanisms of the mammary gland is cited and in Part D the literature on M.haemolytica is reviewed.In the second Chapter, after a brief introduction (Part A), a novel technique to obtain material from the teat duct of ewes is described and validated (Part B). Then, the bacteriological findings in the teat duct and the mammary gland of clinically healthy ewes, as well the effects of milking are presented (Part C). Finally, the histopathological findings in clinically healthy teats of ewes are presented (Part D).In Part B, a new technique that may be used for studying the bacterial flora of the teat duct in live ewes is described; furthermore, evidence regarding its accuracy, safety and repeatability is presented. A sterile, plastic, 20 G catheter is used for sampling. The catheter stylet is taken out and the plastic catheter cut with a sterile blade to a length of 2 mm. In order to ensure accurate and consistent cutting of the catheter at the desired length, a sterilized ruler is placed beside the catheter. The whole procedure is carried out under aseptic conditions, on sterilized surgical screens. Before sampling, a thorough disinfection with povidone iodine scrub solution of the teat apex and the lower (1 cm) part of the teat skin, is carried out. The investigator holds the catheter from the cannula hub; then, it is inserted into the teat, rolled around the internal teat wall, in order to sample the mucosa, and then withdrawn. Material collected on the tip of the catheter is plated onto bacteriological media. The sensitivity of the technique compared to a method that detects the bacterial flora of the teat after slaughter of the animals (scratching of the teat mucosa by means of a scalpel blade) was 99% for field samples and 100% for experimental samples; specificity of the technique was 91% and 100%, respectively; kappacoefficient between results of the two methods was 0.9045. The safety of the technique was 12Institutional Repository -Library & Information Centre -University of Thessaly 11/05/2018 11:26:29 assessed in an investigation, where ewes were repeatedly (eight times) sampled by means of this technique, throughout their lactation; in no case was clinical or subclinical mastitis recorded. Finally, the repeatability of the method was assessed in 37-paired samples, obtained 30 to 40 min. apart; a 100% match was r...

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Binding of a surface protein of Staphylococcus aureus to cultured ovine mammary gland epithelial cells

Cited by 10 publications

References 26 publications

Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis

Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis

Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis

Προστατευτικός ρόλος της θηλής και σημασία των παθήσεων της στην εκδήλωση μαστίτιδας από mannheimia haemolytica σε πρόβατα

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