Binding of bivalent cations by hyaluronate in aqueous solution

Furth, Guido; Knierim, Robert; Buss, Volker; Mayer, Christian

doi:10.1016/j.ijbiomac.2007.09.001

Cited by 35 publications

(18 citation statements)

References 19 publications

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“…As the binding of the cations leads to reduced solution viscosities, one can assume that the complex formation is connected to a reduction of the average hydrodynamic radius. Hence, we conclude that the binding occurs preferably between side chains of the same xanthan molecule, leading to intramolecular complexes comparable to those observed for hyaluronic acid [10]. This interpretation is in accordance with the dependence of the solution viscosity on the shear rate at different ion concentrations: with increasing ion concentration, the shear rate dependence disappears as the internally linked polysaccharide chain loses its ability to undergo shear-induced conformational changes.…”

Section: Model For the Structure Of The Polysaccharide-cation Complexsupporting

confidence: 87%

“…In some cases, the resulting structures show a well-defined crystalline geometry such as the egg-box structure in case of alginate in presence of Ca 2+ [17]. On other occasions such as for hyaluronic acid, the bivalent cation is bound by the formation of an intramolecular complex, a phenomenon which actually leads to a reduction of the hydrodynamic radius of dissolved polysaccharide chains [10].…”

Section: General Backgroundmentioning

confidence: 99%

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Binding of bivalent cations by xanthan in aqueous solution

Bergmann

Furth

Mayer

2008

International Journal of Biological Macromolecules

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Section: Model For the Structure Of The Polysaccharide-cation Complexsupporting

confidence: 87%

Section: General Backgroundmentioning

confidence: 99%

Binding of bivalent cations by xanthan in aqueous solution

Bergmann

Furth

Mayer

2008

International Journal of Biological Macromolecules

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“…In fact, Jeong et al [20] prepared a cisplatin-hyaluronate complex using a hyaluronan at a molecular weight of 100 kD and obtained negatively charged nanoparticles by simple complexation of hyaluronate with cisplatin. This result can be explained by the findings of Furth et al [32] who demonstrated that the interaction between a divalent cation and a hyaluronate at a molecular weight of about 400 kD is strictly intramolecular. Moreover, the release curve of the aforementioned complex showed a remarkable increase of released drug after 48 h and later.…”

Section: Discussionmentioning

confidence: 88%

Preparation, characterisation and preliminary antitumour activity evaluation of a novel nanoparticulate system based on a cisplatin-hyaluronate complex and N-trimethyl chitosan

Cafaggi¹,

Russo²,

Stefani³

et al. 2009

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In this work, nanoparticles with a positive surface charge were prepared through the electrostatic interaction of a new cisplatin-hyaluronate complex with N-trimethyl chitosan (substitution degree of 85%). Mean particle diameter was approximately 195 nm. Drug loading of nanoparticles, which had a zeta potential of about 27 mV, was equal to 6% w/w. After 24 h, while the cisplatin-hyaluronate complex released approximately 60% w/w drug in phosphate buffered saline at pH 7.4, approximately 40% w/w of total cisplatin was released from nanoparticles. The same cumulative amounts of released drug were found after 48 h. These nanoparticles, as well as the starting cisplatin-hyaluronate complex, were active on all cell lines tested (P388, A2780, A549), with an antiproliferative activity similar to that of cisplatin. Apoptosis was markedly induced in A2780 cells by nanoparticles. In a preliminary in vivo experiment, the antitumour activity against a murine tumour (P388 cells) subcutaneously implanted in mice, resulted similar to that of cisplatin for nanoparticles whereas the starting complex showed a non-significant activity at the cisplatin dose tested. Body weight change of treated mice suggested a significantly better tolerance of the nanoparticles compared to cisplatin, after an initial brief period of acute toxicity higher than the parent drug. These results indicate that such a particulate system could be useful as a carrier for cisplatin delivery.

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“…13, 25, 26 On the other hand, HA interactions via Ca 2+ are intramolecular and involve carboxyl and N-acetyl groups of two disaccharide units in a single, folded HA chain. 27 Given the initial MW increase, we consider the HA-Ca interaction to be both intra- and intermolecular (involving 2–4 HA molecules) rather than strictly intramolecular. Further increase of Ca 2+ resulted in “MW” decrease of HA-Ca, which suggested increasing intramolecular interactions of HA (Fig.…”

Section: Discussionmentioning

confidence: 99%

Stabilization of a hyaluronate-associated gene delivery system using calcium ions

et al. 2014

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A “DPH” ternary complex consisting of plasmid DNA (pDNA), intracellularly degradable polyethyleneimine, and hyaluronic acid (HA) is a promising non-viral gene carrier with low toxicity and good gene transfection efficiency. HA plays a key role in providing an optimal balance between DNA protection and release, but it causes aggregation due to the entanglement of HA chains of neighbouring DPH particles. Here we report that the addition of an optimal level of Ca2+ successfully prevents particle aggregation and maintains a relatively small size. The Ca-stabilized DPH is comparable to DPH in cytotoxicity and gene transfection efficiency. MW monitoring and conductometric titration suggest that such size stabilization effect is partly mediated by the complexation between HA and Ca2+, which enables intra- and intermolecular interactions of HAs.

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Binding of bivalent cations by hyaluronate in aqueous solution

Cited by 35 publications

References 19 publications

Binding of bivalent cations by xanthan in aqueous solution

Binding of bivalent cations by xanthan in aqueous solution

Preparation, characterisation and preliminary antitumour activity evaluation of a novel nanoparticulate system based on a cisplatin-hyaluronate complex and N-trimethyl chitosan

Stabilization of a hyaluronate-associated gene delivery system using calcium ions

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