Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients

Delanghe, Sigurd; Biesen, Wim Van; Velde, Nadeige Van de; Eloot, Sunny; Pletinck, Anneleen; Schepers, Eva; Glorieux, Griet; Delanghe, Joris; Speeckaert, Marijn M.

doi:10.1515/cclm-2017-0444

Cited by 19 publications

(6 citation statements)

References 36 publications

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“…Serum albumin levels were measured conferring the bromocresol green dye-binding method on a Roche Modular DP analyser (Roche Diagnostics) (Delanghe et al 2018). Cholesterol levels were measured with the CHOD-PAP method (Barzehkar et al 2016).…”

Section: Serum Albumin and Cholesterol Levelsmentioning

confidence: 99%

The effects of stress reduction strategies of breathing technique relaxation programme and positive psychology on biochemical variable response among schoolteachers

Thephilah

Aruna²,

Vijayaragahavan³

2022

Biosci. J.

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We aimed to investigate the effects of job stress on the biochemical variables of schoolteachers before and after stress reduction strategies. In 2019, 200 schoolteachers (98 males, 102 females) in the private and fully aided higher secondary schools of urban and rural areas in Vellore, India were enrolled in the study based on the principle of consecutive sampling. Questionnaires were used to investigate demographic characteristics and occupational stress-related factors. Fasting blood glucose was detected by venous plasma and a biochemical analyser with the cut-off thresholds method. Salivary cortisol was determined by the specific enzyme-linked immunosorbent assay kit method. Total cholesterol in serum was determined by the CHOD-PAP method. Serumalbumin was detected by the bromocresol green dye-binding method on a Roche Modular DP analyser (Roche Diagnostics). Blood pressure was measured by aneroid sphygmomanometers. Levels of fasting blood glucose, salivary cortisol, albumin and cholesterol were lower in the high-tension group than those in the low-tension group. Those of the high-coping strategy group were higher than those of the low-coping strategy group. Systolic blood pressure and diastolic blood pressure significantly reduced among the intervention groups after administering the relaxation response programme for three and six weeks, respectively, to urban and rural school teachers. Occupational stress can lead to increased salivary cortisol, cholesterol, albumin, blood glucose and blood pressure levels. The relaxation response programme resulted in quantifiable improvements in physiological markers of stress in rural and urban schoolteachers.

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Section: Serum Albumin and Cholesterol Levelsmentioning

confidence: 99%

The effects of stress reduction strategies of breathing technique relaxation programme and positive psychology on biochemical variable response among schoolteachers

Thephilah

Aruna²,

Vijayaragahavan³

2022

Biosci. J.

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“…Confounders comprise carbamylation, 2 uremic toxins (e.g., para-cresyl sulfate, 3 and a 2 -macroglobulin concentration. 4 The altered plasma protein spectrum in nephrotic syndrome (higher a 2 -macroglobulin-albumin ratio) highlights the analytical failure of the dye binding assays. The acute phase protein a 1 -acid glycoprotein 3 affects albumin test results when using bromocresol-based dyes.…”

Section: Albumin Assays and Clinical Decision-making In Nephrotic Synmentioning

confidence: 99%

“…3 In these patients, the performance of BCP methods seems sufficient and would therefore be "fit for intended use." Admittedly, the binding of BCP to albumin is influenced by protein carbamylation 2 and uremic toxins, 4 which has been shown in dialysis patients. The most important lesson is that clinicians should be aware of the pitfalls of chromogenic assays and an adequate alternative measurement method should be used when necessary.…”

Section: Albumin Assays and Clinical Decision-making In Nephrotic Synmentioning

confidence: 99%

Albumin assays and clinical decision-making in nephrotic syndrome patients

Delanghe

Speeckaert

Delanghe

et al. 2019

Kidney International

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“…The effective use of 3,3 -dimethyl-5,5 -dibromophenolsulfonephthalein (BP) in technologies is expanding . It includes sensitive gas sensors [27,28], electrochemical DNA biosensors to the antitumor drug [39], highly efficient extraction of heavy metals from industrial effluents by adsorption on a modified zeolite surface [40], lysine decarboxylase analysis [41], the adsorption of hydrophobic molecules on the membrane of erythroleukemia cells [42], and evaluation of the properties of serum albumin [38,[43][44][45][46][47][48] and extracellular lactase [49]. It is noteworthy that many applications are based on the formation of dissimilar associates between the anionic form of sulfonephthalein and the analyte.…”

Section: Introductionmentioning

confidence: 99%

Cation–Anionic Interactions of Dyes in Aqueous Solutions: Bromocresol Purple in the Processes of Dissimilar Association

Shapovalov¹

2021

Colorants

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The interaction between single- or double-charged anions of bromocresol purple (BP) and cyanine cations (quinaldine blue, QB, or quinaldine red, QR) at concentrations of dyes 5.0·10−7–4.0·10−5 mol/L has been investigated by vis-spectroscopy. The thermodynamic constants of dissimilar associations (Kas) have been studied. Comparison of the values of lg Kas shows that QB− associates of BP− are more stable (6.61 ± 0.07) than QR associates (4.84 ± 0.06); a similar phenomenon is observed for associates of the BP2− anion. Semi-empirical calculations (PM3 method) are in agreement with the vis-spectroscopy data and indicate that the association of dye into an associate is possible. The standard enthalpies of formation of associates (ΔfHo) and energy diagrams have been determined. The ΔfHo data indicate that the formation of an associate between dye ions is an energetically favourable process. The gain in energy significantly exceeds the systematic error of semi-empirical calculations and increases from 157 kJ/mol (associate ”BP− + QB+”) to 729 kJ/mol (associate “BP2− + QR+”). The most probable structures of dissimilar associates are presented. The study of the dissimilar association develops the concept of intermolecular interactions in solutions.

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Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients

Abstract: In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.

Cited by 19 publications

References 36 publications

The effects of stress reduction strategies of breathing technique relaxation programme and positive psychology on biochemical variable response among schoolteachers

The effects of stress reduction strategies of breathing technique relaxation programme and positive psychology on biochemical variable response among schoolteachers

Albumin assays and clinical decision-making in nephrotic syndrome patients

Cation–Anionic Interactions of Dyes in Aqueous Solutions: Bromocresol Purple in the Processes of Dissimilar Association

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