Binding of Curcumin with Bovine Serum Albumin in the Presence of ι-Carrageenan and Implications on the Stability and Antioxidant Activity of Curcumin

Abstract

This work studied the influences of formation of BSA/ι-carrageenan complexes on the binding, stability, and antioxidant activity of curcumin. In the presence of BSA and ι-carrageenan, curcumin gives higher intensities of absorption and fluorescence than free curcumin and curcumin only combined with BSA. The added ι-carrageenan is observed to promote curcumin for quenching the instrinsic fluorescence of BSA. These results are explained in terms of the formation of BSA/ι-carrageenan complexes, which help to stabilize the folded structure of BSA for providing curcumin with a more hydrophobic microenvironment. The small difference in anisotropy values of curcumin with BSA alone and of BSA/ι-carrageenan complexes suggests that ι-carrageenan acts as outer stretch conformation in BSA/ι-carrageenan complexes but does not directly disturb the hydrophobic pockets inside BSA, where curcumin is hydrophobically located. The determined values of the binding constant are higher for curcumin with BSA/ι-carrageenan complexes than with BSA alone. Moreover, BSA/ι-carrageenan complexes are found to be superior to single BSA for enhancing the stability and DPPH radical-scavenging ability of curcumin.

Curcumin encapsulated in the complex of lysozyme/carboxymethylcellulose and implications for the antioxidant activity of curcumin

“…In addition, when Ly and CMC existed in the solutions simultaneously, the intensities of both spectra of Cur strikingly increased. The results indicated that more of Cur was solubilized in Ly solution because there was a hydrophobic microenvironment in Ly, and the Ly-CMC coacervates gave a more hydrophobic region than Ly alone, which agreed well with Yang' report about the BSA/ι-carrageenan complexes to encapsulate Cur (Yang et al, 2013). The largest negative charges of CMC 1.2 produced the largest electrostatic interaction between Ly and CMC.…”

“…Besides, the maximum spectra of Cur in Ly-Cur-CMC coacervates showed a distinct red shift in absorption spectra and a blue shift in fluorescence spectra, compared with those in free Cur and Ly-Cur solutions. The shifts of peaks also confirmed that the environment around Cur was more hydrophobic (Yang et al, 2013).…”

“…The radical-scavenging activity of Cur was examined according to the DPPH method (Yang, Wu, Li, Zhou & Wang, 2013). Briefly, scavenging activity assay was carried out by recording the absorbance of samples containing DPPH at 517 nm in the presence and absence of the remnant Cur at room temperature with a UV-vis spectrophotometer.…”

Investigation of antioxidant activity and release kinetics of curcumin from tara gum/ polyvinyl alcohol active film

“…A major problem is that curcumin is insoluble in water, which limits its application, and therefore, many methods have been used to overcome this barrier to expand its application. Such methods involve using ionic liquid (Luo, Varaprasad, Reddy, Rajulu, & Zhang, 2012) or sodium phosphate buffer (Yang, Wu, Li, Zhou, & Wang, 2013) or dimethylsulfoxide (Bajpai, Chand, Ahuja, & Roy, 2015b) to dissolve curcumin or turn curcumin into curcumin nanoparticles or liposomal curcumin (Raghavendra, Jayaramudu, Varaprasad, Ramesh, & Raju, 2013). However, these methods are expensive and complex.…”

Advances in Study on Structure of Polysaccharide

“…As a result, in the DSC heating curve, endothermic peaks show intermolecular hydrogen bond breaking, GLP20 changed from triple helix structure is a single irregular coiled conformation, and after cooling reheating process, endothermic peak with the increase in the number of scans becomes small gradually, show that more than 135 DEG C heating caused by the conformational transition is irreversible. Differential scanning calorimetry (DSC) can also be senior structure of composite material, such as the application of the technique to study indicated that with the changes in the environment, carrageenan will conformational transition from helical to random coil form, and combined with macromolecular substances, due to the presence of steric hindrance, it cannot occur normal folding [23]. As a result, the DSC curve is larger endothermic peak as the evaluation of a drug delivery system, carrageenan and poly -(dimethylamino) phosphazene compound is stable is an important index [24].…”