Binding of Engeletin with Bovine Serum Albumin: Insights from Spectroscopic Investigations

Abstract

In this paper, several spectroscopic techniques were used to investigate the interaction of engeletin (ELN) with bovine serum albumin (BSA). The analysis of UV-Vis absorption and fluorescence spectra revealed that ELN and BSA formed a static complex ELN-BSA, and ELN quenched the fluorescence of BSA effectively. According to the thermodynamic parameters ΔS(0) = 47.27 J·mol(-1)·K(-1) and ΔΗ(0) = -10.34 kJ·mol(-1), the hydrophobic and hydrogen bond interactions were suggested to be the major interaction forces between ELN and BSA. Raman spectroscopy indicated that the binding of ELN slightly changed the conformations and microenviroment of BSA and decreased the α-helix content of BSA.

Engeletin Alleviates Lipopolysaccharide-Induced Endometritis in Mice by Inhibiting TLR4-mediated NF-κB Activation

“…To date, many Chinese herbal medicines have been widely applied in the treatment of inflammatory diseases, such as mastitis, acute lung injury, and endometritis. ,, Engeletin (dihydrokaempferol 3-rhamnoside, shown in Figure A) is a flavanonol glycoside that can be found in white wine and the skin of white grapes and can be isolated from the bark of Hymenaea martiana. , Engeletin is widely distributed in Southeast Asia, and the leaves are available to the preparation of a sweet tea named huang qi, which facilitates weight control in China, and the Japanese Kohki tea . Several studies have confirmed that engeletin exerts various biological effects, such as anti-inflammatory analgesic, diuresis, detumescence, and antibiosis effects . Although some studies on the anti-inflammatory activity of engeletin and astilbin (dihydroquercetin 3-rhamnoside, another flavanonol glycoside) have been reported, no research has demonstrated the effectiveness of engeletin on LPS-stimulated endometritis.…”

The investigation of the interaction between orientin and bovine serum albumin by spectroscopic analysis

“…Synchronous fluorescence spectra provide information about the molecular microenvironment in the vicinity of the chromophore molecules, and are obtained by simultaneously scanning excitation and emission wavelengths while keeping a constant wavelength difference (Δλ) between excitation and emission. When the difference between the excitation and emission wavelengths is kept constant at 15 or 60 nm, the synchronous fluorescence spectrum gives information characteristic of tyrosine or tryptophan residues .…”

Multi-spectroscopic studies on the interaction of human serum albumin with astilbin: Binding characteristics and structural analysis

“…The intensity ratio of the doublet (I 850 /I 830 ) is useful to detect the microenvironment of tyrosine residues [46]. From the value of this ratio, the information about the hydroxyl groups of tyrosy1 residues (''exposed'' or ''buried'') were obtained [47].…”