The binding of Streptococcus dysgalactiae to fibronectin involves fibronetcin-binding protein(s) present on the bacterial surface. Previously, we reported the cloning of two different genes coding for cell-wall-associated fibronectin-binding proteins from S. dysgalactiae strain S2 [Lindgren, P.-E., Speziale, P., McGavin, M. J., Monstein, H.-J., Hook, M., Visai, L., Kostiainen, T., Bozzini, S. & Lindberg, M. (1992) J. Biol. Chem. 267, 1924-19311. The two genes, fibA and fnbB, have now been sequenced and the primary amino acid sequences of the two fibronectin-binding proteins, FnBA and FnBB, have been deduced. The two proteins have predicted molecular masses of 117 kDa and 122 kDa, respectively, and are organized in a similar way. The fibronectin-binding activities are localized in repeated motifs, 32-37 amino acids long, in the COOH-terminal regions of the proteins. The two fibronectin-binding proteins have heterologous amino acid sequences, except for the COOH-terminal ends which include the fibronectin-binding repeats. The fibronectin-binding regions of the genes have been fused to IgG-binding domains of protein A, utilizing the IgG-binding capacity of the resulting fusion proteins, to facilitate isolation of the fibronectin-binding domains.Fibronectin (Fn) is a large dimeric glycoprotein (molecular mass 430-470 kDa) which is found in plasma and other body fluids in a soluble form, and is incorporated as microfibrils into the extracellular matrix of most tissues as an insoluble form. The main function of Fn is presumably related to its ability to mediate the substrate adhesion of eucaryotic cells, which involves the binding of specific cell-surface receptors of the integrin family to certain domains in the Fn molecule. The first characterized binding site in Fn for eucaryotic cells contains the amino acid sequence RGD and is located in the central region of the protein (Pierschbacher and Ruoslahti, 1984). Recently, additional recognition sites have been identified (Komoriya et al., 1991). Fn also interacts with other matrix components ; binding sites for collagen, fibrin and sulfated glycosaminoglycans are located in discrete domains of the protein. The present knowledge of the structure and function of Fn has recently been summarized by Hynes (1990).