Binding of glycated ovocystatin to rat renal brush border membranes

Abstract

Glycated proteins are considered as one of the factors involved in the pathogenesis of diabetic complications, including nephropathy. These proteins are formed endogenously under conditions of hyperglycemia, as well as being provided with food containing sugars, which was subjected to high temperature. Examples are egg products. One of the proteins found in eggs in a relatively high concentration is chicken cystatin (ovocystatin). It is now believed that some proteins can passage the intestinal epithelium by transcytosis directly into the bloodstream. Thus, glycated protein present in food can be an additional source of glycotoxins. The aim of this study was to compare the affinity of native and glycated cystatin to the brush border membranes of rat kidney. Kinetic analysis was performed with surface plasmon resonance technique using sensor chip L1. Dissociation constants for native and glycated cystatin (Kd ) were 2.76 μmol/L and 3.82 μmol/L, respectively. The results of our study indicate that glycation only slightly affects binding of cystatin to brush border membranes. This suggests that glycated cystatin and other glycated proteins may also be efficiently taken up in the kidney proximal tubule. The observation may be important for understanding the mechanisms involved in the development of diabetic nephropathy.

Influence of Piracetam on Gliclazide—Glycated Human Serum Albumin Interaction. A Spectrofluorometric Study

“…Due to the presence of AGEs in diabetic patients’ blood, in the late complications of diabetes (retinopathy, nephropathy, neuropathy, and coronary atherosclerosis) not only hyperglycemia, but also glycosylated connections take place. This fact has been confirmed by the scientific studies on the animal models and in vitro glycated albumin coming from human or bovine [5,6,7,8]. AGEs can modulate the signaling pathways in the cell and influence the inflammation mediators involved in the pathomechanism of diabetic complications [9].…”