Binding of mercury in the rat kidney by metallothionein

Wiśniewska, Justyna; Trojanowska, B; Piotrowski, J.; Jakubowski, M.

doi:10.1016/0041-008x(70)90081-5

Cited by 101 publications

(18 citation statements)

References 10 publications

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“…Thus, it is necessary to modify the set of biomarkers to be able to capture a broader range of different types of biological effects. Among others, these should include endpoints indicative of the exposure to metals, such as metallothioneins (Wisniewska et al 1970), and endocrine disruptors, such as vitellogenin or markers of steroidogenesis (Jones et al 2000), respectively. Furthermore, histological investigation of ultrastructural changes should be assessed as indicators of tissue damage or alterations (Arnold et al 1996a, b;Grund et al 2010).…”

Section: Identification Of Suitable Biomarkers For Use In Coupled Hydmentioning

confidence: 99%

A combined hydraulic and toxicological approach to assess re-suspended sediments during simulated flood events. Part I–multiple biomarkers in rainbow trout

et al. 2010

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Purpose One of the central issues related to global changes in weather is the increasing occurrence of flood events that can result in the re-suspension of contaminated sediments in rivers. Here, we report on a proof-of-concept study combining hydraulic engineering and ecotoxicology in a new interdisciplinary approach to assess the toxicity of resuspended polluted sediments after a simulated flood event. Materials and methods Rainbow trout (Oncorhynchus mykiss) were exposed for 5 days under simulated flood conditions in an annular flume with artificial sediments that were spiked with a mixture of polycyclic aromatic hydrocarbons (PAH) at environmentally relevant concentrations.Specifically, the objective of this study was to bridge the gap between the physical re-suspension of pollutants and resulting toxicological impacts on aquatic organisms. A suite of different molecular, biochemical and histological markers was used to test the hypothesis that re-suspension of sediments can lead to re-mobilization of PAHs and subsequently to effects on aquatic organisms. Results and discussion The micronucleus frequency was significantly 4.3-fold elevated after exposure. There was no significant indication of Aryl hydrocarbon receptor signaling (no EROD induction or increased CYP1A protein content, only slight induction of CYP1A gene expression). Biliary metabolite concentration was the most sensitive Responsible editor:

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Section: Identification Of Suitable Biomarkers For Use In Coupled Hydmentioning

confidence: 99%

A combined hydraulic and toxicological approach to assess re-suspended sediments during simulated flood events. Part I–multiple biomarkers in rainbow trout

et al. 2010

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“…However, as soon as this constant environment is disturbed, these tissues also respond--usual1y by modulating functions and expressing genes that are not supposed to be modulated or expressed. An example of this phenomenon is the induction of meta110thioneins, the binding proteins which are usually found only in liver and kidney~vivo (Wisniewska~~, 1970;Squibb and Cousins, 1977). Yet, regardless of tissue of origin, cells in culture ++ ++ once exposed to Cd or Zn are capable of producing the protein (Shaikh and Lukis, 1971;Webb and Daniel, 1955;Failla and Cousins, 1978;Rudd and Herschman, 1979;Hildebrand.~~, 1979).…”

Section: Quantity As a Tissue Specific And Developmental Markermentioning

confidence: 99%

The Differentiated State of Normal and Malignant Cells or How to Define a “Normal” Cell in Culture

Bissell

1981

International Review of Cytology

198

112

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“…These proteins are induced in the liver and kidneys of animals in response to sublethal doses of certain heavy metals (Bremner & Young, 1976;Nordberg et al, 1972;Shaikh & Lucis, 1971;Weser et al, 1973;Winge & Rajagopalan, 1972;Wisniewska et al, 1970), and their appearance is accompanied by enhanced incorporation of radioactively labelled amino acids into metallothionein fractions (Bremner & Davies, 1974;Cherian & Clarkson, 1976;Piotrowski et al, 1974;Richards &Cousins, 1975a,b).…”

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confidence: 99%

Induction of cadmium-thionein in isolated rat liver cells

Hidalgo¹,

Koppa²,

Bryan³

1978

Biochemical Journal

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The uptake of cadmium by isolated liver cells was linearly related to the cadmium concentration to which the cells were exposed in the medium. Cadmium-treated cells synthesized proteins de novo with the characteristics of cadmium-thionein induced in the liver ofcadmium-treated animals. Thionein from liver cells incorporated cadmium and[35S]cysteine, had a Vl/Vo (Sephadex G-50) of 1.8-1.9, and was separated into two subfractions by DEAE-cellulose ion-exchange chromatography. Cycloheximide and actinomycin D when added after a cadmium exposure prevented the synthesis ofthionein. However, addition of actinomycin D after synthesis had started only decreased the total amount of thionein synthesized. The concentration of cadmium to which the cells were exposed affected the amount of cadmium-thionein synthesized in 6h. The maximum response occurred when cells were exposed to 0.5,ug of cadmium/ml; at higher metal concentrations the total amount of cadmium-thionein synthesized declined. The system described in the present paper can be used to study the mode of metal toxicity and the mechanism of cadmium-thionein synthesis.

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Binding of mercury in the rat kidney by metallothionein

Cited by 101 publications

References 10 publications

A combined hydraulic and toxicological approach to assess re-suspended sediments during simulated flood events. Part I–multiple biomarkers in rainbow trout

A combined hydraulic and toxicological approach to assess re-suspended sediments during simulated flood events. Part I–multiple biomarkers in rainbow trout

The Differentiated State of Normal and Malignant Cells or How to Define a “Normal” Cell in Culture

Induction of cadmium-thionein in isolated rat liver cells

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