Binding of Mucin by E. coli from Human Gut

Вахрушева, Т. В.; Baikova, Julia; Балабушевич, Н. Г.; Гусев, С. А.; Lomakina, G. Yu.; Sholina, Ekaterina A; Moshkovskaya, Mariam; Shcherbakov, Peter; Pobeguts, Olga; Mikhalchik, Elena V.

doi:10.1007/s10517-018-4137-3

Cited by 8 publications

(8 citation statements)

References 12 publications

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“…Then, lymphocytes are prevented from migrating and homing from the blood to inflammatory tissue, ultimately inhibiting intestinal inflammation. Vedolizumab, a representative anti-adhesion molecular agent, shows intestinal specificity and can specifically antagonize intestinal α4β7 integrin heterodimers, but its effect is relatively slow, with a clinical response time of at least 2 wk[ 121 ]. A study has shown that the clinical remission rate of patients with UC was 47.1% and that the mucosal healing rate was 40.9% after 6 wk of treatment with vedolizumab[ 122 ].…”

Section: Aiec In Ucmentioning

confidence: 99%

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

Duan¹,

Dai²,

Wu³

2022

World J Clin Cases

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Gut microbiota imbalances play an important role in inflammatory bowel disease (IBD), but no single pathogenic microorganism critical to IBD that is specific to the IBD terminal ileum mucosa or can invade intestinal epithelial cells has been found. Invasive Escherichia coli ( E. coli) adhesion to macrophages is considered to be closely related to the pathogenesis of inflammatory bowel disease. Further study of the specific biological characteristics of adherent invasive E. coli (AIEC) may contribute to a further understanding of IBD pathogenesis. This review explores the relationship between AIEC and the intestinal immune system, discusses the prevalence and relevance of AIEC in Crohn's disease and ulcerative colitis patients, and describes the relationship between AIEC and the disease site, activity, and postoperative recurrence. Finally, we highlight potential therapeutic strategies to attenuate AIEC colonization in the intestinal mucosa, including the use of phage therapy, antibiotics, and anti-adhesion molecules. These strategies may open up new avenues for the prevention and treatment of IBD in the future.

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Section: Aiec In Ucmentioning

confidence: 99%

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

Duan¹,

Dai²,

Wu³

2022

World J Clin Cases

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“…The aim of the current study was to evaluate the effect of mucin adsorption by E. coli cells on neutrophil activation in vitro . We previously demonstrated that E. coli isolates from the healthy or inflamed (Crohn's disease) human intestine and the laboratory strain DH5α were able to adsorb mucin from a diluted solution in vitro . Among our findings, the amount of adsorbed mucin varied greatly between E. coli isolates (more than threefold).…”

mentioning

confidence: 47%

“…Among our findings, the amount of adsorbed mucin varied greatly between E. coli isolates (more than threefold). No significant difference in the ability to adsorb mucin was observed between the isolates from the inflamed and healthy intestine and the laboratory strain .…”

mentioning

confidence: 82%

“…Bacteria (0.09 mg DW) were incubated in 1 mL of 0.15 m NaCl containing 0.1 mg·mL −1 mucin (or without mucin) at 25 °C for 1 h. After centrifugation (20 min, 900 g ), the mucin concentration in the supernatants was assessed by absorption at 214 nm ( A 214 ), with 0.1 mg·mL −1 mucin solution used as a reference sample . From the difference in these concentrations, the amount of adsorbed mucin was calculated.…”

Section: Methodsmentioning

confidence: 99%

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Mucin adsorbed by E. coli can affect neutrophil activation in vitro

et al. 2019

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Bacteria colonizing human intestine adhere to the gut mucosa and avoid the innate immune system. We previously demonstrated that Escherichia coli isolates can adsorb mucin from a diluted solution in vitro. Here, we evaluated the effect of mucin adsorption by E. coli cells on neutrophil activation in vitro. Activation was evaluated based on the detection of reactive oxygen species production by a chemiluminescent reaction (ChL), observation of morphological alterations in neutrophils and detection of exocytosis of myeloperoxidase and lactoferrin. We report that mucin adsorbed by cells of SharL1 isolate from Crohn's disease patient's inflamed ileum suppressed the potential for the activation of neutrophils in whole blood. Also, the binding of plasma complement proteins and immunoglobulins to the bacteria was reduced. Desialylated mucin, despite having the same adsorption efficiency to bacteria, had no effect on the blood ChL response. The effect of mucin suggests that it shields epitopes that interact with neutrophils and plasma proteins on the bacterial outer membrane. Potential candidates for these epitopes were identified among the proteins within the bacterial outer membrane fraction by 2D‐PAGE, fluorescent mucin binding on a blot and HPLC‐MS/MS. In vitro, the following proteins demonstrated mucin adsorption: outer membrane porins (OmpA, OmpC, OmpD and OmpF), adhesin OmpX, the membrane assembly factor OmpW, cobalamine transporter, ferrum uptake protein and the elongation factor Ef Tu‐1. In addition to their other functions, these proteins are known to be bacterial surface antigens. Therefore, the shielding of epitopes by mucin may affect the dynamics and intensity of an immune response.

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“…(6) Mucins of the intestinal mucosa are high molecular weight glycoproteins consisting of 80% carbohydrates and 20% protein. (7) Although the intestines are usually considered anaerobic, the tissues surrounding the lumen are quite rich in oxygen and oxygen diffuses into the intestines and, in addition, oxygen is present in the swallowed air. That is, the intestines are not strictly anaerobic; therefore, the ability to have aerobic respiration provides E. coli with a great competitive advantage.…”

Section: Introductionmentioning

confidence: 99%

The Study of the Growth of Escherichia coli on Pectins

Shubakov¹,

Mikhailova²

2019

IJBM

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The growth of E. coli on pectins isolated from various plant sources was studied. We used commercial apple pectin AU701 and low-methyl esterified pectins (6-22%) from callus cultures of tansy Tanacetum vulgare L. (TV, tanacetan), duckweed Lemna minor L. (LM, lemnan), and campion Silene vulgaris (M.) G. (SV, silenan). Bacterial growth was also tested on the enzymatic degradation products of tanacetan pectin. For comparison, bacterial growth was studied on easily metabolizable carbon sources-glucose and lactose. E. coli was cultivated on solid media in Petri dishes and in liquid nutrient media in Erlenmeyer flasks at a temperature of +37°C and at room temperature. It was found that E. coli colonies do not form with growth on gels of tanacetan, lemnan and silenan. When growing on a gel of apple pectin, a weak bacterial growth is detected. However, E. coli is capable of growth on soluble products of enzymatic hydrolysis of tanacetan pectin-oligogalacturonides.

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Binding of Mucin by E. coli from Human Gut

Cited by 8 publications

References 12 publications

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

Mucin adsorbed by E. coli can affect neutrophil activation in vitro

The Study of the Growth of Escherichia coli on Pectins

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