Binding of porphyrin to horseradish peroxidase: Effects on structure and function

Real-time molecular recognition between protein and photosensitizer of photodynamic therapy by quartz crystal microbalance sensor

“…Among the three THPP isomers (o-, m-, and p-THPP), o-THPP is toxic to skin, whereas m-THPP and p-THPP [18,19] have been shown to possess high photosensitivity and organic selectivity and been used as PDT photosensitizers for anticancer treatment. Spectrofluorometric and spectrophotometric methods are usually used for the study of the interaction between protein and PDT photosensitizers [20][21][22]. Compared with these two traditional methods, a QCM sensor exhibits unique advantages such as miniaturization, less sample consumption, short analysis time, and reusability.…”